A5100440331- Genetics, Aging, and Longevity in Model Organisms
- Cellular transport and secretion
- Photoreceptor and optogenetics research
- Circadian rhythm and melatonin
- Pancreatic function and diabetes
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Retinal Development and Disorders
- Spine and Intervertebral Disc Pathology
- Lipid Membrane Structure and Behavior
- Neurobiology and Insect Physiology Research
- Synthesis and biological activity
- Musculoskeletal pain and rehabilitation
- Bioactive Compounds and Antitumor Agents
- Hippo pathway signaling and YAP/TAZ
- Fungal and yeast genetics research
- Cancer therapeutics and mechanisms
- Plant Gene Expression Analysis
- Microtubule and mitosis dynamics
- Molecular Sensors and Ion Detection
- Galectins and Cancer Biology
- Neurogenesis and neuroplasticity mechanisms
- Single-cell and spatial transcriptomics
- Medical Imaging and Analysis
City University of Hong Kong
2025
Qingdao University
2021-2025
Affiliated Hospital of Qingdao University
2021-2025
Jiaxing University
2024
Park Centre for Mental Health
2017-2023
The University of Queensland
2017-2023
Huazhong University of Science and Technology
2014-2023
Sichuan University
2021-2023
Tongji University
2021-2023
Yangzhou University
2021-2023
Calorie restriction (CR), the only non-genetic intervention known to slow aging and extend life span in organisms ranging from yeast mice, has been linked down-regulation of Tor, Akt, Ras signaling. In this study, we demonstrate that serine/threonine kinase Rim15 is required for chronological extension caused by deficiencies Ras2, Tor1, Sch9, calorie restriction. Deletion stress resistance transcription factors Gis1 Msn2/4, which are positively regulated Rim15, also a major although not...
Fluorescent dextrans are commonly used as macropinocytic probes to study the properties of endocytic cargoes; however, effect size on mechanisms has not been carefully analyzed. By using chemical and siRNA inhibition individual pathways, we evaluated internalization two dextrans, Dex10 (dextran 10 kDa) Dex70 70 kDa), in mammalian HeLa cells Caenorhabditis elegans coelomocytes. We revealed that enters these cell types predominantly via clathrin- dynamin-independent amiloride-sensitive...
The advent of O-arm navigation optimized the oblique lumbar interbody fusion (OLIF) procedure, allowing operator to simultaneously perform OLIF and percutaneous posterior pedicle screw implantation without patient position change, thus improving fluency accuracy procedure (called as OLIF360). Nevertheless, a consensus regarding its suitability for patients with severe spinal stenosis remains elusive. This study aims investigate clinical efficacy OLIF360 imaging changes in cases....
Munc13 plays a crucial role in short-term synaptic plasticity by regulating vesicle (SV) exocytosis and neurotransmitter release at the presynaptic terminals. However, intricate mechanisms governing these processes have remained elusive due to presence of multiple functional domains within Munc13, each playing distinct roles release. Here we report coordinated mechanism C. elegans homolog UNC-13 that controls switch during transmission. Mutations disrupting interactions C1 C2B with...
Munc13 plays a crucial role in short-term synaptic plasticity by regulating vesicle (SV) exocytosis and neurotransmitter release at the presynaptic terminals. However, intricate mechanisms governing these processes have remained elusive due to presence of multiple functional domains within Munc13, each playing distinct roles release. Here we report coordinated mechanism C. elegans homolog UNC-13 that controls switch during transmission. Mutations disrupting interactions C1 C2B with...
Munc13 plays a crucial role in short-term synaptic plasticity by regulating vesicle (SV) exocytosis and neurotransmitter release at the presynaptic terminals. However, intricate mechanisms governing these processes have remained elusive due to presence of multiple functional domains within Munc13, each playing distinct roles release. Here, we report coordinated mechanism Caenorhabditis elegans homolog UNC-13 that controls switch during transmission. Mutations disrupting interactions C1 C2B...
Ganglioside GM1-bound cholera toxin-B sub-unit (CT-b) enters the cell via clathrin-coated pits and dynamin-independent non-caveolar raft-dependent endocytosis. Caveolin-1 (Cav1), associated with caveolae formation, is a negative regulator of In mammary epithelial tumour cells deficient for Mgat5, Cav1 stably expressed at levels below threshold forming stable oligomerized microdomains or scaffolds that were shown to suppress EGFR signalling reduce plasma membrane diffusion rate both CT-b....
The locomotion of C. elegans is balanced by excitatory and inhibitory neurotransmitter release at neuromuscular junctions. However, the molecular mechanisms that maintain balance synaptic transmission remain enigmatic. Here, we investigated function voltage-gated Ca2+ channels in triggering spontaneous cholinergic GABAergic synapses. Recordings miniature excitatory/inhibitory postsynaptic currents (mEPSCs mIPSCs, respectively) showed UNC-2/CaV2 EGL-19/CaV1 are two major triggers for release....
The degeneration characteristics of adjacent segment disease are complex. Improper surgical planning has caused unnecessary trauma and costs. purpose this study was to establish a staging system for evaluate its guiding significance decisions in disease. A retrospective performed on 103 patients with who underwent treatment between January 2017 2023. Based radiological findings, categorized into four stages, no cases identified Stage IV. Patients were divided intervention groups: Group...
Munc13 plays a crucial role in short-term synaptic plasticity by regulating vesicle (SV) exocytosis and neurotransmitter release at the presynaptic terminals. However, intricate mechanisms governing these processes have remained elusive due to presence of multiple functional domains within Munc13, each playing distinct roles release. Here we report coordinated mechanism C. elegans homolog UNC-13 that controls switch during transmission. Mutations disrupting interactions C1 C2B with...
Significance Understanding how clathrin-independent endocytic (CIE) cargo proteins are sorted into and transported within endosomes has attracted growing interests. Here, in Caenorhabditis elegans intestine, a well-established genetic system for deciphering trafficking multicellular organisms, we report direct visualization of dynamic endosomal tubules as carriers basolateral recycling certain CIE cargoes. We unveil the coordination Rab GTPase RAB-10, exocyst component SEC-10, microtubule...
UNC-13 proteins play an essential role in synaptic transmission by recruiting vesicles (SVs) to become available for release, which is termed SV priming. Here we show that the C2A domain of UNC-13L, like corresponding mammalian Munc13-1, displays two conserved binding modes: forming C2A/C2A homodimers, or a heterodimer with zinc finger UNC-10/RIM (C2A/RIM). Functional analysis revealed UNC-13L’s promotes regulating post-priming process. Stimulus-evoked release but not priming, was impaired...
Significance Rapid communication between neurons relies on the precise coordination of several presynaptic active-zone proteins. Munc13/UNC-13 is centerpiece this deeply conserved process, and domains large cytoplasmic protein serve specific roles such as coordinating SNARE assembly, integrating calcium lipid signals, bringing synaptic vesicles close to plasma membrane, although a mechanistic understanding these critical steps lacking. Here, we describe for first time novel C2 domain (HC2M)...
Abstract Disturbed inhibitory synaptic transmission has functional impacts on neurodevelopmental and psychiatric disorders. An essential mechanism for modulating is alteration of the postsynaptic abundance GABA A Rs, which are stabilized by scaffold proteins recruited presynaptic signals. However, how GABAergic neurons trigger signals to transsynaptically recruit Rs remains elusive. Here, we show that UNC-43/CaMKII functions at modulate C. elegans neuromuscular junctions. We demonstrate...
Ca2+-dependent neurotransmitter release requires synaptotagmins as Ca2+ sensors to trigger synaptic vesicle (SV) exocytosis via binding of their tandem C2 domains—C2A and C2B—to Ca2+. We have previously demonstrated that SNT-1, a mouse synaptotagmin-1 (Syt1) homologue, functions the fast sensor in Caenorhabditis elegans. Here, we report new sensor, SNT-3, which triggers delayed release. snt-1;snt-3 double mutants abolish evoked transmission, demonstrating C. elegans NMJs use dual system....
Munc13 proteins play several roles in regulating short-term synaptic plasticity. However, the underlying molecular mechanisms remain largely unclear. Here we report that C. elegans UNC-13L, a Munc13-1 ortholog, has three domains inhibit vesicle (SV) exocytosis. These include X (sequence between C2A and C1), C1, C2B domains. Deleting all inhibitory produces hyperactive UNC-13 (sUNC-13) exhibits dramatically increased neurotransmitter release, Ca2+ sensitivity of release probability. The...
The development of functional synapses in the nervous system is important for animal physiology and behaviors, its disturbance has been linked with many neurodevelopmental disorders. synaptic transmission efficacy can be modulated by environment to accommodate external changes, which crucial reproduction survival. However, underlying plasticity remains poorly understood. Here we show that Caenorhabditis elegans, male increases hermaphrodite cholinergic at neuromuscular junction (NMJ), alters...
Synapses exhibit multiple forms of short-term plasticities, which have been attributed to the heterogeneity neurotransmitter release probability. However, molecular mechanisms that underlie differential states remain be fully elucidated. The Unc-13 proteins appear key roles in synaptic function through regulatory domains. Here, we report deleting M domain Caenorhabditis elegans UNC-13MR leads a significant increase probability, revealing an inhibitory this domain. effect is eliminated when...
Sexually dimorphic behaviors are ubiquitous throughout the animal kingdom. Although both sex-specific and sex-shared neurons have been functionally implicated in these diverse behaviors, less is known about roles of neurons. Here, we discovered sexually cholinergic synaptic transmission C. elegans occurring at neuromuscular junctions (NMJs), with males exhibiting increased release frequencies, which result locomotion behaviors. Scanning electron microscopy revealed that significantly more...
Synaptotagmin-1 (Syt1) binds Ca<sup>2+</sup> through its tandem C2 domains (C2A and C2B) triggers Ca<sup>2+</sup>-dependent neurotransmitter release. Here, we show that <i>snt-1</i>, the homolog of mammalian Syt1, functions as sensor for both tonic evoked release at <i>Caenorhabditis elegans</i> neuromuscular junction. Mutations disrupt binding in double SNT-1 significantly impaired release, whereas disrupting a single domain had no effect, indicating two is functionally redundant...