A5103040284- Dialysis and Renal Disease Management
- Endoplasmic Reticulum Stress and Disease
- Renal Diseases and Glomerulopathies
- Muscle and Compartmental Disorders
- Organ Donation and Transplantation
- Organ Transplantation Techniques and Outcomes
- Parathyroid Disorders and Treatments
- Blood properties and coagulation
- Heme Oxygenase-1 and Carbon Monoxide
- Biomedical Research and Pathophysiology
- Erythropoietin and Anemia Treatment
- Cell Adhesion Molecules Research
- Renal and Vascular Pathologies
- NF-κB Signaling Pathways
- Immune Response and Inflammation
- Advanced Glycation End Products research
- Venous Thromboembolism Diagnosis and Management
- Vitamin C and Antioxidants Research
- Adipokines, Inflammation, and Metabolic Diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Genetic and Kidney Cyst Diseases
- Sirtuins and Resveratrol in Medicine
- Magnesium in Health and Disease
- Iron Metabolism and Disorders
- Cytokine Signaling Pathways and Interactions
Asan Medical Center
2004-2021
Ulsan College
2001-2021
University of Ulsan
2007-2021
University of Michigan
2009
Hanyang University Medical Center
2008
LG Chem (South Korea)
1998
Biotechnology Research Institute
1998
McGill University
1988
Because loss of podocytes associates with glomerulosclerosis, monitoring podocyte by measuring products in urine may be clinically useful. To determine whether a single episode injury would cause persistent loss, we induced limited depletion using diphtheria toxin receptor (hDTR) transgenic rat. We monitored detecting nephrin and podocin mRNA particulates quantitative reverse transcriptase–PCR. Aquaporin 2 served as kidney reference gene to account for variable contribution RNA amount...
It has been suggested that endoplasmic reticulum (ER) stress facilitates fibrotic remodeling. Therefore, modulation of ER may serve as one the possible therapeutic approaches to renal fibrosis. We examined whether and how activation AMP-activated protein kinase (AMPK) suppressed induced by chemical inducers [tunicamycin (TM) thapsigargin (TG)] also nonchemical in tubular HK-2 cells. further investigated vivo effects AMPK on Western blot analysis, immunofluorescence, small interfering (si)RNA...
The epithelial-mesenchymal transition (EMT) is a novel mechanism that promotes renal fibrosis. Transforming growth factor-β (TGF-β), angiotensin II, aldosterone, high glucose, and urinary albumin are well-known causes of EMT We examined whether how activation AMP-activated protein kinase (AMPK) suppressed induced by the above agents in tubular epithelial cells. All experiments were performed using HK-2 Protein expression was measured Western blot analysis. Intracellular reactive oxygen...
Technical Briefs The Formulation of Dynamical Equations Holonomic Mechanical Systems Using a Natural Orthogonal Complement Jorge Angeles, Angeles Department Engineering, Robotic Laboratory, McGill Research Centre for Intelligent Machines, University, Montreal, Quebec, Canada H3A 2A7 Search other works by this author on: This Site PubMed Google Scholar Sang Koo Lee Author and Article Information J. Appl. Mech. Mar 1988, 55(1): 243-244 (2 pages) https://doi.org/10.1115/1.3173642 Published...
The epithelial-mesenchymal transition (EMT) and endoplasmic reticulum (ER) stress induced by urinary protein, particularly albumin, play an important role in tubulointerstitial injury. However, signaling pathways regulating both albumin-induced EMT ER are not precisely known. We postulated that reactive oxygen species (ROS), c-Src kinase, mammalian target of rapamysin (mTOR) would act as upstream molecules. further examined the effect imatinib mesylate on these processes. All experiments...
Endoplasmic reticulum (ER) stress has been implicated in inducing epithelial-mesenchymal transition (EMT). ER is also known to induce autophagy. However, it unclear whether stress-induced autophagy contributes EMT. We hypothesized that might EMT through via activation of c-Src kinase tubular epithelial cells.All experiments were performed using HK-2 cells. Protein expression was measured by Western blot analysis. Immunofluorescence and small interfering RNA (siRNA) performed.Chemical...
<b><i>Background:</i></b> Endoplasmic reticulum (ER) stress induced by urinary albumin plays an important role in tubulointerstitial injury. We have shown that albumin-induced ER is regulated through reactive oxygen species (ROS)-c-Src kinase-mTOR signaling pathways. postulated peroxisome proliferator-activated receptor-γ (PPAR-γ) might also act as upstream molecule between c-Src kinase and mTOR. It has been suggested AMP-activated protein (AMPK) involved attenuation...
Endoplasmic reticulum (ER) stress is emerging as an important factor in the development of organ fibrosis. Therefore, modulation ER may serve one possible therapeutic approaches to renal SIRT1, a class III histone deacetylase, has been found exert beneficial effects kidney diseases. However, it largely unknown whether and how SIRT1 suppresses stress. We postulated that upregulation would suppress through induction heme oxygenase-1 (HO-1) thioredoxin.HK-2 tubular cells, experimental mouse...
Background. Endothelial cells are active participants in the processes controlling coagulation, inflammation and immune response. Variations recognized between endothelia isolated from different vascular beds as well species. Though transforming growth factor‐β1 (TGF‐β1) has been known to have an anti‐inflammatory action, little is about its effect on expression of cellular adhesion molecules during inflammatory process human glomerular endothelial cells. The aim this study was determine...
Oxidative stress plays various roles in the development and progression of IgA nephropathy, while bilirubin is known as a potent antioxidant. We therefore hypothesized that serum would be associated with renal prognosis nephropathy. The study subjects comprised 1,458 adult patients primary nephropathy Korea. grouped according to following quartile levels bilirubin: <0.4 mg/dL (Q1), 0.4-0.5 (Q2), 0.6-0.7 (Q3), >0.8 (Q4). outcome data were obtained from Korean Registry end-stage disease...
Significance Statement Steroid resistance, relapse, and side effects are common issues in use of high-dose steroids as first-line treatment for adult minimal change nephrotic syndrome. Tacrolimus is used a steroid-sparing immunosuppressant to reduce adverse long-term or repeated steroid treatment, but no large-scale randomized study has compared combined tacrolimus low-dose with treating syndrome adults. In this open-label trial, the authors found that plus was noninferior complete remission...
To investigate the effect of interleukin-1beta (IL-1beta) on production extracellular matrix in cultured human peritoneal mesothelial cells (HPMCs).Cultured HPMCs were treated with or without IL-1beta. Cell morphology was observed. The expression fibronectin, alpha1(I) procollagen, and transforming growth factor-beta1 (TGFbeta1) mRNAs measured by Northern blot analysis. cell surface fibronectin type I collagen evaluated immunofluorescent staining. Fibronectin culture supernatant inhibition...
Activation of nuclear factor-κB (NF-κB) occurs by dissociation from IκB after serine or tyrosine phosphorylation IκBα, but the way NF-κB activation high glucose has not been defined. High is known to activate via protein kinase C and reactive oxygen species (ROS). In this study, we investigated how activates for CC chemokine ligand 2 production in cultured human glomerular endothelial cells. increased translocation p65 also DNA binding activity. glucose-induced occurred without degradation...
<b><i>Background: </i></b>We compared the accuracy of Modification Diet in Renal Disease (MDRD) study and Chronic Kidney Epidemiology Collaboration (CKD-EPI) equations Korean patients evaluated difference CKD prevalence determined using two general population. <b><i>Methods: </i></b>The was 607 who underwent a chromium-51-ethylenediaminetetraacetic acid GFR measurement. Additionally, we by among 5,822 participants fifth Korea National Health...
<i>Background:</i> The ratio of bioimpedance in the right leg (rl-RBI) may be helpful adjusting dry body weight (DBW) new hemodialysis (HD) patients. <i>Methods: </i>rl-RBI was calculated as follows: rl-RBI = impedance at 50 kHz/impedance 500 kHz, measured by spectroscopy (BIS). Theoretically, is inversely related to extracellular water. A reference range obtained from 137 chronic but stable HD patients already achieving DBW. In 34 (females:males 16:18; age 49 ± 12...
Thrombin, a crucial enzyme in the blood coagulation, has been target for antithrombotic therapy. Orally active thrombin inhibitors would provide effective and safe prophylaxis venous arterial thrombosis. We conducted optimization of highly efficacious benzamidine-based inhibitor LB30812 (3, K(i) = 3 pM) to improve oral bioavailability. Of variety arylamidines investigated at P1 position, 2,5-thienylamidine effectively replaced benzamidine without compromising inhibitory potency absorption....
Leukocyte adhesion to mesothelium is an important step during peritonitis, which mediated by molecules including vascular cell molecule-1 (VCAM-1). We investigated the effect of exogenous nitric oxide (NO) on VCAM-1 expression in cultured human peritoneal mesothelial cells and its signal transduction pathway. Mesothelial were exposed tumor necrosis factor-alpha (TNF-alpha) presence or absence NO donors, 3-morpholino-sydnonimine (SIN-1) nitroprusside (NP). mRNA protein measured Northern blot...