Whey proteins (WP) exert anti-inflammatory and antioxidant effects. Hyperbaric pressurisation of whey increases its digestibility changes the spectrum peptides released during digestion. We have shown that dietary supplementation with pressurised improves nutritional status systemic inflammation in patients cystic fibrosis (CF). Both clinical indices are largely affected by airway processes, to which respiratory epithelial cells actively contribute. Here, we tested whether from digestion can...

Whey proteins have well-established antioxidant and anti-inflammatory bioactivities. High hydrostatic pressure processing of whey protein isolates increases their

The bioactivity of dietary polyphenols depends upon gastrointestinal and hepatic metabolism secondary microbial phenolic metabolites generated via colonic microbiota-mediated biotransformation. A polyphenol-rich potato extract (PRPE) containing chlorogenic, caffeic, ferulic acids rutin was digested in a dynamic multi-reactor simulator the human intestinal ecosystem (GI model). Simulated digestion showed extensive degradation parent compounds generation metabolites. To characterize transport...

Objective: This study aimed to assess the impact of OHIP subscale on OHRQoL and compare adolescents adults. Material Methods: A total 160 subjects (60 100 adults) undergoing orthodontic treatment completed Malaysian Oral Health Impact Profile (S-OHIP(M)) OHRQoL. The instrument consisted 14 items, grouped into 7 subscales, which were: (i) functional limitation, (ii) physical pain, (iii) psychological discomfort, (iv) disability, (v) (vi) social (vii) handicap. Mann-Whitney U Kruskal Wallis...

A dynamic human gastrointestinal (GI) model was used to digest cooked tubers from purple-fleshed Amachi and Leona potato cultivars study anthocyanin biotransformation in the stomach, small intestine colonic vessels. Colonic Caco-2 cancer cells non-tumorigenic CCD-112CoN were tested for cytotoxicity cell viability after 24 h exposure fecal water (FW) digests (0%, 10%, 25%, 75% 100% FW culture media). After digestion, liquid chromatography-mass spectrometry identified 36 15 species throughout...

Cystic fibrosis (CF) is characterized by malnutrition, chronic pulmonary inflammation, and oxidative stress. Whey protein rich in sulfhydryl groups recognized for its ability to increase glutathione reduce Previously, we have shown that supplementation with whey increased intracellular levels patients CF. We subsequently hyperbaric pressure treatment of promotes the release novel peptides absorption, increases healthy subjects, reduces vitro production interleukin (IL)-8. hypothesized...

Both sexes of mice were fed a high fat diet ( HFD ) for 10 weeks without and with polyphenolic‐rich potato extracts PRPE cultivars Onaway Russet Burbank. attenuated weight gain in male female by as much 63.2%, which was associated mostly reduction adiposity. Mice receiving showed enhanced capacity blood glucose clearance. Sex differences regarding the impact on plasma levels insulin, ghrelin, leptin, gastric inhibitory peptide, resistin noted. may serve part preventative dietary strategy...

Probiotic supplementation plays a key role in maintaining intestinal homeostasis due to its ability modulate gut microbiota. Although their potential as potent antioxidants have previously been explored, affect the redox status lumen of healthy subjects or those with gastrointestinal (GI) disorders remains unclear. In our study, we assessed single strain and multispecies probiotic cause change normal fecal water Clostridium (C.) difficile-infected using simulated model. Changes were by...

<i>Background:</i> High-dose ibuprofen is clinically effective in cystic fibrosis (CF); however, its molecular mechanisms are poorly understood. <i>Objective:</i> To test the hypothesis that relevant concentrations of suppress activation nuclear factor (NF)-ĸB and thus down-regulate stimulated interleukin (IL)-8 production CF respiratory epithelial cells. <i>Methods:</i> The majority experiments were conducted CFTE29o– cells (F508del-mutated transmembrane...

Clostridium (C.) difficile-infection (CDI), a nosocomial gastrointestinal disorder, is of growing concern due to its rapid rise in recent years. Antibiotic therapy CDI associated with disrupted metabolic function and altered gut microbiota. The use probiotics as an adjunct being studied extensively their potential modulate functions the In present study, we assessed ability several single strain probiotic mixture change normal C. difficile-infected fecal samples. production short-chain fatty...

Down-regulation of overabundant interleukin (IL)-8 present in cystic fibrosis (CF) airways could ease excessive neutrophil burden and its deleterious consequences for the lung. IL-8 production airway epithelial cells, stimulated with e.g. inflammatory cytokines IL-1β tumor necrosis factor (TNF)-α, is regulated by several signaling pathways including nuclear (NF)-κB p38 mitogen-activated protein kinase (MAPK). We previously demonstrated that anti-inflammatory drugs dexamethasone ibuprofen...

Osteoarthritis (OA), the most common form of arthritis, is associated with metabolic diseases and gut microbiome dysbiosis. OA patients often take supplements collagen hydrolysates (CHs) a high peptide content. Following digestion, some peptides escape absorption to induce prebiotic effects via their colonic fermentation generate short-chain fatty acids (SCFAs), branched-chain (BCFAs) gases (NH4 H2S). The capacity CHs microbial metabolites unknown. Proteomic analysis two (CH-GL CH-OPT)...

Potatoes (Solanum tuberosum L.) are a good dietary source of carbohydrates in the form digestible starch (DS) and resistant (RS). As increased RS content consumption can be associated with decreased chronic disease risk, breeding efforts have focused on identifying potato varieties higher content, which requires high-throughput analysis profiles. For this purpose, freeze drying potatoes has been used but approach leads to inaccurate values. The present study objective was assess (RS, DS...

Clostridioides difficile infection (CDI) is frequently associated with intestinal injury and mucosal barrier dysfunction, leading to an inflammatory response involving neutrophil localization upregulation of pro-inflammatory cytokines. The severity clinical manifestations the extent immune response, which requires mitigation for better management. Probiotics could play a protective role in this disorder due their immunomodulatory ability gastrointestinal disorders. We assessed five...