A consumer-grade fused filament fabrication (FFF) 3D printer was used to construct fluidic devices for nanoparticle preparation and electrochemical sensing. Devices were printed using poly(ethylene terephthalate) featured threaded ports connect polyetheretherketone (PEEK) tubing via fittings prepared from acrylonitrile butadiene styrene (ABS). These included channels designed have 800 μm × square cross sections semitransparent allow visualization of the solution-filled channels. 3D-printed...

We report here the fabrication and validation of a novel 3D-printed, automated immunoarray to detect multiple proteins with ultralow detection limits. This low cost, miniature employs electrochemiluminescent (ECL) measured CCD camera touch-screen control micropump facilitate use. The miniaturized array features prefilled reservoirs deliver sample reagents paper-thin pyrolytic graphite microwell chip complete sandwich immunoassays. achieves high sensitivity by using single-wall carbon...

Ultrasensitive, quick, and non-invasive recognition of biomarkers is very useful for detection, screening, management cancer effectively. In this regard, there have been enormous number articles published each year comprising natural biomolecules e.g., antibodies, enzymes, receptors aptamers as a element sensors the detection biomarkers. However, due to stability issue high-cost biomolecules, polymeric material-based involving molecular imprinting has drawn huge attention over last couple...

Monoclonal antibodies (mAb) are key components of revolutionary new disease immunotherapies and also essential for diagnostics imaging. However, natural (Abs) very expensive, storage in harsh conditions is challenging, stability over extended periods remains a bottleneck. A material-based substitute to Abs could address all these associated restrictions. Thus, the development artificial (A-Abs) via molecular imprinting (MI) has recently drawn enormous attention. Several reviews were...

We have developed a method that for the first time allowed us to synthesize silica particles in 20 minutes using sol-gel preparation. Therefore, it is critically important understand synthesis mechanism and kinetic behavior order achieve higher degree of fine tuning ability during synthesis. In this study, we employed our modulate physical nature reaction medium from emulsion, which has halt at particular time; precisely chemistry polymerization. The kept quite simple with tetraethyl...

Natural antibodies are used widely for important applications such as biomedical analysis, cancer therapy, and directed drug delivery, but they expensive may have limited stability. This study describes synthesis of antibody-like binding sites by molecular imprinting on silica nanoparticles (SiNP) using a combination four organosilane monomers with amino acid-like side chains providing hydrophobic, hydrophilic, H-bonding interactions target proteins. approach provided artificial antibody...

A cost-effective and robust biomimetic magnetic nanomaterial based on surface molecular imprinting similar to antibody-like biorecognition sites for chiral drug molecules has been demonstrated. The importance of separation drugs recognized because one the enantiomers often causes undesirable side effects. In this article, we have imprinted (S)-naproxen, a nonsteroidal antiinflammatory that is used treat pain, inflammation, fever, using nanoparticles. Acrylamide ethylene glycol dimethacrylate...

Quartz nanopipettes have recently been employed for resistive-pulse sensing of Au nanoparticles (AuNP) and with bound antibodies. The analytical signal in such experiments is the change ionic current caused by nanoparticle translocation through pipette orifice. This paper describes detection cancer biomarker (Vascular Endothelial Growth Factor-C, VEGF-C) use antibody-modified AuNPs nanopipettes. main challenge was to differentiate between attached antibodies VEGF-C antigen-conjugated...

Rapid and selective detection of microorganisms in complex biological systems draws huge attention to address the rising issue antimicrobial resistance. Diagnostics based on identification whole are laborious, time-consuming costly, thus alternative strategies for early clinical diagnosis include biomarker microbial detection. This paper describes a low-cost, easy-to-use method Pseudomonas aeruginosa infections by specifically identifying pyocyanin, using surface-molecularly imprinted...

Nowadays, silica nanoparticles are gaining tremendous importance because of their wide applications across different domains such as drug delivery, chromatography, biosensors, and chemosensors. The synthesis generally requires a high percentage composition organic solvent in an alkali medium. eco-friendly bulk amounts can help save the environment is cost-effective. Herein, efforts have been made to minimize concentration solvents used during via addition low electrolytes, e.g., NaCl....

Staphyloccus aureus (S. aureus) capture and detection using protein A surface imprinted magnetic nanoparticles.

Polymerized films synthesized on magnetic nanoparticles (NP) using amino acid-like monomers yielded reusable NPs that efficiently remove HSA from serum.

Understanding of the synthesis kinetics and our ability to modulate medium conditions allowed us generate nanoparticles via an ultra-fast process. The is kept quite simple with tetraethyl orthosilicate (TEOS) as precursor 50% ethanol sodium hydroxide catalyst. Synthesis performed under gentle at 20 °C for min Long time catalyst-associated drawbacks are most crucial in silica nanoparticle synthesis. We have addressed both these bottlenecks by replacing conventional Stober catalyst, ammonium...

Damage to DNA from the metabolites of drugs and pollutants constitutes a major human toxicity pathway known as genotoxicity. Metabolites can react with metal ions NADPH oxidize or participate in SN2 reactions form covalently linked adducts bases. Guanines are main oxidation sites, 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxodG) is initial product. Here we describe novel electrochemiluminescent (ECL) microwell array that produces test compounds measures relative rates adduct damage. In this new...

Sugars' sense of zinc: A glucose-based naphthylimino conjugate (L) has been synthesized and structurally characterized. L shown to exhibit selective turn-on fluorescence towards Zn2+ in an aqueous alcoholic medium. The zinc complex, turn, recognizes phosphates, nucleotides, DNA by turn-off fluorescence, which further investigated molecular dynamics simulations. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not...

Monoclonal antibodies (mAbs) are key components of revolutionary disease immunotherapies and also essential for medical diagnostics imaging. The impact cost is illustrated by a price >$200,000 per year patient mAb-based cancer therapy. Purification represents major issue in the final these immunotherapy drugs. Protein A (PrA) resins widely used to purify antibodies, but resin cost, separation efficiency, reuse, stability issues. This paper explores synthesis strategy low-cost, reusable,...

The COVID-19 pandemic has caused over 7 million deaths worldwide and 1 in the US as of October 15, 2022. Virus testing lags behind level or availability necessary for events like COVID-19, especially resource-limited settings. Here, we report a low cost, mix-and-read assay using synthetic SARS-CoV-2 sensor, imaged processed smartphone. was optimized saliva employs 3D-printed micropipette tips with layer monoclonal anti-SARS-CoV-2 inside tip. A polymeric sensor spike (S) protein (COVRs)...

Decreasing costs, improving availability, and the introduction of new materials for 3D printing technologies have resulted in more extensive applications chemical biochemical research. Recently, has been used to make microfluidic devices mixing, gradient generation, sensing applications, including electrochemical via flow-injection amperometry. While several reports 3D-printed appeared, most these rely on polyjet, multijet, or stereolithography methods that require expensive printers, costly...