The analysis of cargo proteins in exosome subpopulations has considerable value diagnostics but a translatable impact been limited by lengthy or complex extraction protocols. We describe herein scalable, fast, and low-cost using an alternating (AC) magnetic field to support the dynamic mixing antibody-coated beads (MBs) with serum samples within 3D-printed microfluidic chips. Zwitterionic polymer-coated MBs are, specifically, magnetically agitated ultraclean capture efficiencies >70% from...

Electrocatalysis of water is a scalable and easily available source the production hydrogen (H2), future energy carrier. This drive for clean inspired us to develop an inexpensive, readily producible, highly active, stable catalyst replace current state art platinum catalysts. Building on promising evolution reaction (HER) activity many pyrites, their structural tuning by different metals nonmetals has been found be effective in several instances. We present here one such effort partial...

Abstract The development of wearable, all‐in‐one sensors that can simultaneously monitor several hazard conditions in a real‐time fashion imposes the emergent requirement for smart and stretchable avoidance sensing platform is skin‐like. Multifunctional with these features are problematic challenging to accomplish. In this context, multimodal ferrofluid‐based triboelectric nanogenerator (FO‐TENG), featuring capabilities variety stimulus such as strong magnetic field, noise level, falling or...

We report here the influence of antibody immobilization strategy for protein immunosensors on screen printed carbon electrode arrays in terms binding activity, analytical sensitivity, limit detection, and stability. Horseradish peroxidase (HRP) was model analyte with anti-HRP immobilized sensors, HRP activity used detection. Covalently antibodies electrodes coated chitosan, electrochemically reduced graphene oxide (rGO), dense gold nanoparticle (AuNP) films had only 20-30% total active...

Circulating tumour cells (CTCs) are cancer shed from a primary which intravasate into the blood stream and have potential to extravasate distant tissues, seeding metastatic lesions. As such, they can offer important insight progression with their presence generally associated poor prognosis. The detection enumeration of CTCs is, therefore, critical guiding clinical decisions during treatment providing information on disease state. CTC isolation has been investigated using plethora...

We report herein a novel pipet-based "ELISA in tip" as new versatile diagnostic tool featuring better sensitivity, shorter incubation time, accessibility, and low sample reagent volumes compared to traditional ELISA. Capture analysis of data by cell phone facilitates electronic delivery results health care providers. Pipette tips were designed 3D printed adapters fit most commercial 50–200 μL pipettes. antibodies (Ab1) are immobilized on the inner walls pipet tip, which serves assay...

The inability to distinguish aggressive from indolent prostate cancer is a longstanding clinical problem. Prostate specific antigen (PSA) tests and digital rectal exams cannot differentiate these forms. Because only ∼10% of diagnosed cases are aggressive, existing practice often results in overtreatment including unnecessary surgeries that degrade patients' quality life. Here, we describe fast microfluidic immunoarray optimized determine 8-proteins simultaneously 5 μL blood serum for...

AbstractWhile the technology is relatively new, low cost 3D printing has impacted many aspects of human life. printers are being used as manufacturing tools for a wide variety devices in spectrum applications ranging from diagnosis to implants external prostheses. The ease use and availability design software made an accessible fabrication tool research laboratories. can print materials with varying density, optical character, strength chemical properties providing platforms huge number...

A 3D-printed microfluidic array detects 2 proteins in 4 samples at very low cost with downloadable plans

A microfluidic open circuit potential label-free protein assay was developed for the reagentless quantification of C-reactive (CRP), a model target, and further utilized to assess target-receptor binding kinetics. Generated sensors have very high baseline stabilities (<1% change in 100 min) levels selectivity complex media. Real-time assays are fast (<20 min), sensitivity (1 ng/mL limit detection CRP serum), resolve kinetic thermodynamic characteristics that correlate well with those...

The development of real-life applicable ion sensors, in particular those capable repeat use and long-term monitoring, remains a formidable challenge. Herein, we demonstrate, proof-of-concept, the real-time voltammetric sensing anions under continuous flow 3D-printed microfluidic system. Electro-active anion receptive halogen bonding (XB) hydrogen (HB) ferrocene-isophthalamide-(iodo)triazole films were employed as exemplary sensory interfaces. Upon exposure to anions, cathodic perturbations...

We introduce a facile assessment of binding kinetics at bioreceptive redox-active interfaces as means quantifying target proteins. This is achieved by monitoring the redox capacitance (Cr) receptor-modified conductive polymer interface under continuous flow. Exemplified with quantification C-reactive protein (CRP), analyses resolve both association and dissociation regimes in real-time. Significantly, rate electrochemical signal change within regime sensitive function concentration, enabling...

Hepatitis C Virus (HCV) is an asymptomatic chronic infection with serious clinical consequences. Timely and sensitive detection of HCV RNA critical for control, treatment response follow up. While current technologies, such as PCR or isothermal amplification-based strategies, are specific, they expensive, labor intensive, time consuming limiting their use in field small laboratories.

The progressive emergence of protein biomarkers promises a revolution in the healthcare industry and shift focus from disease management to much earlier intervention. Here, we introduce facile shotgun tagging ensemble proteins clinically relevant media prior specific target capture at antibody-modified electrodes. This facilitates convenient voltammetric quantification markers down sub-pg/mL levels across several orders concentration. A translation methodology an automated microfluidic...

first_page Download PDF settings Order Article Reprints Font Type: Arial Georgia Verdana Size: Aa Line Spacing:  Column Width:  Background: Open AccessAbstract Conductive Mesh Electrodes for Electrochemical Biosensors † by Mohamed SharafeldinMohamed Sharafeldin SciProfiles Scilit Preprints.org Google Scholar Department of Chemistry, University Otago, P.O. Box 56, Dunedin 9054, New Zealand Presented at the 4th International Electronic Conference on Biosensors, 20–22 May 2024; Available...

We introduce a novel electrochemical protein quantitation based on the shotgun biotin tagging of proteins prior to their interfacial immunocapture and polymeric enzyme tagging. The highly amplified faradaic signals generated from ferrocene-tyramine adduct enable fg mL-1 (attomolar) levels detection span cross 5 orders magnitude dynamic range. This work supports ultrasensitive marker in single antibody immunoassay format.

The development of real-life applicable ion sensors, in particular those capable repeat use and long-term monitoring, remains a formidable challenge. Herein, we demonstrate, proof-of-concept, the real-time voltammetric sensing anions under continuous flow at electroactive anion receptive halogen bonding (XB) hydrogen (HB) ferrocene-isophthalamide-(iodo)triazole interfaces. Upon exposure to anions, cathodic perturbations ferrocene redox-transducer are monitored by square-wave voltammetry...

A semi-automated multiple biomarker-based diagnostic microfluidic device for on-line capture and detection of prostate cancer biomarkers is described herein. In the United States, lifetime risk men developing 1 in 7. Strategies staging include specific antigen (PSA) test, digital rectal exam (DRE) biopsy. These practices often fall short terms sensitivity, specificity inability to distinguish between aggressive indolent forms cancer. Inaccurate sensitivity can lead unnecessary treatments...