- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- Biofuel production and bioconversion
- Plant tissue culture and regeneration
- Glycosylation and Glycoproteins Research
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Protist diversity and phylogeny
- Genetic Mapping and Diversity in Plants and Animals
- Plant and fungal interactions
- Enzyme Production and Characterization
- Algal biology and biofuel production
- Biocrusts and Microbial Ecology
- Plant-Microbe Interactions and Immunity
- Fungal Biology and Applications
- Endoplasmic Reticulum Stress and Disease
- Plant Disease Resistance and Genetics
- Marine Toxins and Detection Methods
- Lipid metabolism and biosynthesis
- Essential Oils and Antimicrobial Activity
Anhui Agricultural University
2006-2024
The efficacy of eleven essential oils (EOs) against Aspergillus flavus NRRL 3357 was investigated. highest antifungal activity this aflatoxigenic fungus exhibited by cinnamon, oregano and lemongrass, which showed low minimum inhibitory concentration (MIC) values under vapor conditions. Interactions the three EOs were evaluated fractional inhibition index (FICI), composite (CEO) synergistic activities. Chemical analysis oregano, lemongrass (COL-CEO) revealed that (Z)-citral (33.44%),...
The basic leucine zipper (bZIP) is an important transcription factor required for fungal development, nutrient utilization, biosynthesis of secondary metabolites, and defense against various stresses. Aspergillus flavus a major producer aflatoxin opportunistic fungus on wide range hosts. However, little known about the role most bZIP genes in A. flavus. In this study, we developed high-throughput gene knockout method based Agrobacterium-mediated transformation system. Gene construction by...
A recent algicidal mode indicates that fungal mycelia can wrap and eliminate almost all co-cultivated algal cells within a short time span. However, the underlying molecular mechanism is rarely understood. We applied proteomic analysis to investigate process of Trametes versicolor F21a identified 3,754 proteins. Of these, 30 enzymes with endo- or exoglycosidase activities such as β-1,3-glucanase, α-galactosidase, α-glucosidase, alginate lyase chondroitin were significantly up-regulated....
Abstract Background Fungus infection in staple grains affects the food storage and threatens security. The Aspergillus flavus is known to infect multiple produce mycotoxin Aflatoxin B1, which mutagenic, teratogenic causes immunosuppression animals. However, molecular mechanism of maize resistance A. largely unknown. Results Here we used corn kernels investigate genes using genome-wide association study (GWAS) 313 inbred lines. We characterized levels after inoculating with . GWAS 558,529...
Kernels are important reproductive organs in maize, yet there is a lack of systematic investigation on the differences composition endophytic microorganisms plants from population perspective. We aimed to elucidate developing maize kernels, emphasizing among various inbred lines. The transcriptomic data 368 lines were used explore and diversity microorganisms. findings revealed higher abundance fungi than bacteria followed by protozoa, while viruses less abundant. There significant relative...
Although several regulatory pathways have been reported for Aspergillus flavus, the regulation of aflatoxin production and mycelial growth under different temperatures remains unclear. In this study, A. flavus differentially expressed genes (DEGs) were analyzed three temperatures, by strand-specific RNA-Seq. Results show that a total 2,428 1,474 DEGs identified in fungal mycelia cultured at 20°C 37°C, respectively, as compared with control (28°C). Approximately ~ 79% 37°C samples...
Abstract Aspergillus flavus is a fungus that produces aflatoxin B1, one of the most carcinogenic secondary metabolites. Understanding regulation mechanism biosynthesis in this requires precise methods for genomic integration mutant alleles. To avoid disadvantage DNA into genome by non-homologous or ectopic recombination, we developed novel strategy site-specific foreign using carboxin-resistant sdh2R allele (His 249 Leu). Our results demonstrated transformants were generated with high...
Aspergillus flavus is notorious for contaminating food with its secondary metabolite—highly carcinogenic aflatoxins. In this study, we found that exogenous nitric oxide (NO) donor could influence aflatoxin production in A. flavus. Flavohemoglobins (FHbs) are vital functional units maintaining homeostasis and crucial normal cell function. To investigate whether endogenous NO changes affect biosynthesis, two FHbs, FHbA FHbB, were identified study. was confirmed as the main protein to maintain...