A5056529961- Renin-Angiotensin System Studies
- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hormonal Regulation and Hypertension
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
- Mast cells and histamine
- Dermatologic Treatments and Research
- Pharmacological Effects and Assays
- Metabolomics and Mass Spectrometry Studies
- Urticaria and Related Conditions
- Porphyrin and Phthalocyanine Chemistry
- Luminescence and Fluorescent Materials
- Molecular Sensors and Ion Detection
- Ferroptosis and cancer prognosis
- Neurobiology and Insect Physiology Research
- Systemic Sclerosis and Related Diseases
- Advanced Proteomics Techniques and Applications
University of Southern Denmark
2020-2025
Universidade Federal de Minas Gerais
2019-2021
ABSTRACT Background The renin-angiotensin system involves many more enzymes, receptors and biologically active peptides than originally thought. With this study, we investigated whether angiotensin-(1-5) [Ang-(1-5)], a 5-amino acid fragment of angiotensin II, has biological activity, through which receptor it elicits effects. Methods effect Ang-(1-5) (1µM) on nitric oxide release was measured by DAF-FM staining in human aortic endothelial cells (HAEC), or Chinese Hamster Ovary (CHO) stably...
With the discovery of protective arm renin-angiotensin system (RAS), interest has grown in RAS-related receptors such as angiotensin AT2-receptor [AT2R] potential new drug targets. While it is known that AT2R couple to Gi, also apparent they do not signal via inhibition adenylyl cyclase/decrease cAMP, many Gi-coupled receptors. Thus, standard commercially-available assays cannot be applied test for agonistic or antagonistic properties ligands. This lack hampered development drugs targeting...
Herein, we present the light-induced synthesis and characterization of a La3+/spiropyran derivative complex (LaMC) its application as catalyst when incorporated into electrospun polycaprolactone (PCL) fibers. In addition to experimental methods, computational calculations were also essential better understand structure electronic characteristics LaMC. The LaMC was identified 10-coordinated with La3+ ion coordinated by four oxygens from phenolate carbonyl carboxyl acid group both MC ligands...
This commentary on the article "Relative affinity of angiotensin peptides and novel ligands at AT1 AT2 receptors" by Sanja Bosnyak et al. (Clini. Sci. (Lond.) (2011) 121(7): 297-303. https://doi.org/10.1042/CS20110036) summarises main findings study, followed a discussion their relevance for various aspects biology receptors renin-angiotensin system in context current state knowledge.
ABSTRACT Background and purpose Bradykinin [BK-(1-9)] is an endogenous peptide involved in many physiological pathological processes, such as cardiovascular homeostasis inflammation. The central dogma of the kallikrein-kinin system that BK-(1-9) fragments are biologically inactive. In this manuscript, we proposed to test whether these were indeed Experimental Approach Nitric oxide (NO) was quantified human, mouse rat cells loaded with DAF-FM after stimulation BK-(1-9), BK-(1-7), BK-(1-5)...
Introduction: Our group demonstrated that Angiotensin-(1-5) [Ang-(1-5)] is a biologically active component of the protective arm Renin-Angiotensin System (RAS), displaying high efficacy towards AT 2 R. However, signaling mechanisms for Ang-(1-5) in vitro are still unknown. Objective: To characterize signature human aortic endothelial cells. Methods: Human cells (HAEC) were stimulated with (1μM) 1, 3, 5 or 20 minutes. Proteins isolated, digested and labeled TMTpro TM 16-plex. Phosphorylation...
Introduction: Recombinant human ACE2 increases the circulating levels of angiotensin-(1–5) [Ang-(1–5)], a peptide thus far regarded as biologically inactive. Since is central component protective RAS, we hypothesized that Ang-(1–5) new active within this hormonal system. Objective: To investigate biological activity and signaling mechanisms Ang-(1–5). Methods: In order to show effect test whether signals through AT 2 -receptor (AT R), nitric oxide (NO) release was measured by DAF-FM...
The angiotensin AT2 -receptor (AT2 R) is a key component within the protective arm of renin-angiotensin system inducing vasodilation by nitric oxide (NO) production. Currently, no high-throughput assay available to identify R agonists in vitro, which could explain low number selective ligands drug development programs.To design and validate method for detection activation vitro based on NO release.Human aortic endothelial cells (HAEC) were seeded into 96-well plates at density 5000 per well....
Introduction: Recombinant human ACE2 increases the circulating levels of angiotensin-(1-5) [Ang-(1-5)], a peptide thus far regarded as biologically inactive. Since is central component protective RAS, we hypothesized that Ang-(1-5) new active within this hormonal system. Objective: To investigate biological activity and signaling mechanisms Ang-(1-5). Methods: In order to show effect test whether signals through AT 2 -receptor (AT R), nitric oxide (NO) release was measured by DAF-FM...
Objective: Bradykinin [BK-(1–9)] is an endogenous peptide involved in many physiological and pathological processes, such as cardiovascular homeostasis inflammation. The central dogma of the kallikrein-kinin system that BK-(1–9) fragments are biologically inactive. In this work, we proposed to test whether these were indeed Design method: Nitric oxide (NO) was quantified human, mouse rat cells loaded with DAF-FM after stimulation BK-(1–9), BK-(1–7), BK-(1–5) BK-(1–3). We used adult male...
The angiotensin AT2-receptor (AT2R) is a key component within the protective arm of renin-angiotensin system (RAS), being involved in nitric oxide (NO) production and vasodilation. To this date, no quantitative high-throughput assay available to identify AT2R agonists vitro , which may be reason for low number selective ligands drug development programs. Objective: design validate method detection activation . Methods Results: NO release was selected as readout transfected (CHO-AT2)...