The HBB gene encodes the β-globin protein, one of two main components adult hemoglobin A (HbA) responsible for oxygen transport. β-thalassemia is a genetic disorder caused by mutations affecting chain synthesis, leading to reduced or absent production, impaired erythropoiesis, and generally results in anemia. In this study, human-induced pluripotent stem cell line (hiPSC) MURAi006-A was generated from male fetal skin fibroblasts carrying both β⁰-thalassemia mutation at codon 17 (A > T) 26 (G...

Multiple labs have reported that mammalian ovaries contain oogonial stem cells (OSCs), which can differentiate into oocytes fertilize to produce offspring. However, the physiological relevance of these observations adult ovarian function is unknown. Here we performed targeted and reversible ablation premeiotic germ undergoing differentiation in transgenic mice expressing suicide gene, herpes simplex virus thymidine kinase (HSVtk), driven by promoter stimulated retinoic acid gene 8 (Stra8), a...

Embryo morphology and morphokinetics have been studied for their association with euploid embryos. However, the results are controversial, especially in advanced-aged women group, when risk of aneuploidy increases significantly. This prospective cohort study evaluated between embryo development day-3 cleavage day-5 blastocyst stages euploidy rates, determined using preimplantation genetic testing (PGT-A). Embryos from aged 35 years above who underwent intracytoplasmic sperm injections PGT-A...

Through the use of parabiosis in mice, aging-related deterioration skeletal muscle and liver has been linked to a loss systemic factors that support adult stem or progenitor cell activity. Since ovarian failure recently attributed, at least part, de-novo oocyte-containing follicle formation associated with declining oogonial activity, herein we tested mice if changes influence size reserve. Ovaries young (2-month-old) females parabiotically joined for 5 weeks possess comparable numbers...

Solid surface vitrificaition (SSV) is a cryoperservative method that has been used in the cryopreservation of oocytes, and embryos. Here, we report an application SSV human spermatozoa. We compared with standard freezing terms sperm motility, morphology, vitality DNA integrity. Sperm motility was determined by computer assisted semen analysis, morphology were eosin-methylene blue staining, integrity TUNEL assay. found while both cryopreservative methods produced spermatozoa comparable gave...

Abstract Aim The aim of this study was to examine the effects estradiol valerate ( EV ) on thickness clomiphene citrate CC )‐stimulated endometrium. Material and Methods Thirty‐four normal ovulatory women were randomized double‐blindly into two groups receive 100 mg/day day 2–6 treatment cycle, either vitamin B (placebo) or 6 10–14 cycle. endometrial thickness, pattern, numbers mature follicles, maximal diameters preovulatory follicles evaluated by transvaginal sonographic examination....

Progressive loss of ovarian estrogen (E2) production is a hallmark feature of, if not driving force behind, reproductive aging and the menopause.Recent genetic studies in mice have shown that female germline or oogonial stem cells (OSCs) contribute to maintenance adult function fertility under physiological conditions through support de-novo oogenesis.Here we show mouse OSCs express E2 receptor-α (ERα).In presence E2, ERα interacts with stimulated by retinoic acid gene 8 (Stra8) promoter...

Human iPSC line MU011.A-hiPS was generated from homozygous α-thalassemia (-(SEA)/-(SEA)) fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 contained in three episomal vectors were delivered electroporation.

Abstract The presence of pelvic pain, a pelvic/paravaginal mass, and purulent vaginal discharge in primigravida should raise the possibility obstructed hemivagina uterine didelphys. Though conservative management could result successful pregnancy outcomes, early excision septum adequate drainage offer shorter course complication avoidance.

A key event in human development is the establishment of erythropoietic progenitors bone marrow, which accompanied by a fetal-to-adult switch hemoglobin expression. Understanding this could lead to medical application, notably treatment sickle cell disease and β-thalassemia. The changes gene expression progenitor cells as they migrate from fetal liver colonize marrow are still rather poorly understood, primary (FL) tissues difficult obtain.We obtained FL tissue adult peripheral blood (AB)...

In controlled ovarian hyperstimulation cycles, 15% of oocytes have been proven to be immature. Key factors include failure in signal transmission from the cumulus cell oocyte, insufficient level luteinizing hormone, and internal conditions oocyte itself.The aim present study was investigate effect human chorionic gonadotropin (hCG) on vitro maturity partially cumulus-denuded immature collected after stimulation for fertilization (IVF).This a prospective, randomized design at department...

OHVIRA syndrome is a rare condition usually present during early puberty, after menarche. The main symptoms are caused by the obstruction of hemivagina and accumulation blood, which cause such as dysmenorrhea, chronic pelvic pain or mass. Transvaginal septectomy best way to treat patient’s obstructive hemivagina. However, in young patients, small vagina canal significant limitation, it challenging explore surgical field. Due uncommon at ages variation coexisting anomalies, approach...

In this cross-sectional prospective study, advanced next-generation sequencing technology was used to compare the molecular karyotyping of individual human sperm cells in infertile couples with severe oligoteratozoospermia (i.e., low count and motility) those normal semen. Fourteen who were patients at Ramathibodi Hospital Bangkok, Thailand, recruited from January November 2023, they categorized into two groups based on semen analysis results. The study group comprised oligoteratozoospermia,...

The aim is to study the relation and distribution in gene expression level of luteinizing hormone receptor (LHR) regulator G-protein signaling 2 (RGS2) with oocyte maturation.This cross-sectional was undertaken an instruction-based tertiary care infertility unit, department obstetrics gynecology.After controlled ovarian hyperstimulation, cumulus granulosa cells (CCs) from 59 oocytes among 18 women being treated by vitro fertilization/intracytoplasmic sperm injection cycle technique November...

Background and objective Preimplantation genetic diagnosis (PGD) is a technique to identify the defects in embryos created through vitro fertilization (IVF). The purpose of this technology assist reproduction one or both biological parents carrying known abnormalities. Herein, we report on Thai couple having an experience loss first child affected by neurodegeneration died at age 2. Brain MRI revealed tiger-eye-sign, which was suspected for pantothenate kinase-associated (PKAN). DNA...

The aim of this study was to compare semen parameters and sperm DNA fragmentation (SDF) explore the relationship between SDF 2 7 days abstinence a short period (within 4 hours) in oligozoospermic infertile patients.

การทำนายความสำเรจของการฉดอสจเขาโพรงมดลกดวยจำนวนอสจทเคลอนทหลงการเตรยมนำเชอ อกฤษฎ โตวเศษ MD.*, ฉตรชย ตรธรรมพนจMD.*, มธชพร สขประเสรฐ วชาญ โชคธนะศร ชลธชา สถระพจน , MD.* * คณะแพทยศาสตรโรงพยาบาลรามาธบด มหาวทยาลยมหดล กรงเทพมหานคร ประเทศไทย 10400 วตถประสงค : เพอศกษาความสมพนธระหวางจำนวนอสจทเคลอนทหลงการเตรยมนำเชอและการตงครรภของการฉดอสจเขาโพรงมดลก วธดาเนนการวจย เกบบนทกขอมลจากเวชระเบยนผปวยระหวาง สงหาคม 2556 จนถง มกราคม 2558 ผปวยทมอายนอยกวา35ป มบตรยากโดยไมสามารถหาสาเหตได จะนำมาวเคราะหผล...