A5067630607- Hemoglobinopathies and Related Disorders
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Invertebrate Immune Response Mechanisms
- Cancer-related gene regulation
- Erythrocyte Function and Pathophysiology
- Renal and related cancers
- Porphyrin Metabolism and Disorders
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- Aquaculture disease management and microbiota
- Porphyrin and Phthalocyanine Chemistry
- Autophagy in Disease and Therapy
- Male Reproductive Health Studies
- Photodynamic Therapy Research Studies
- CAR-T cell therapy research
- Adipose Tissue and Metabolism
- Lysosomal Storage Disorders Research
- Kruppel-like factors research
- Neurogenetic and Muscular Disorders Research
- Retinoids in leukemia and cellular processes
- Calpain Protease Function and Regulation
- Insect symbiosis and bacterial influences
- Aquaculture Nutrition and Growth
Ramathibodi Hospital
2015-2024
Mahidol University
2012-2024
National Center for Genetic Engineering and Biotechnology
2006-2012
Prince of Songkla University
2006-2007
Uppsala University
2006
The microsporidian Enterocytozoon hepatopenaei was first described from Thailand in 2009 farmed, indigenous giant tiger shrimp Penaeus (Penaeus) monodon. natural reservoir for the parasite is still unknown. More recently, a closely resembling it morphology and tissue preference found Thai-farmed, exotic, whiteleg (Litopenaeus) vannamei exhibiting white feces syndrome (WFS). Our objective to compare newly pathogen with E. determine its causal relationship WFS.Generic primers used amplify...
Introduction . The Infrapatellar fat pad (IPFP) represents an emerging alternative source of adipose-derived mesenchymal stem cells (ASCs). We compared the characteristics and differentiation capacity ASCs isolated from IPFP SC. Materials Methods were harvested either or IPFPs collected patients undergoing total knee arthroplasty (TKA), whereas subcutaneous tissues lipoaspiration. Immunophenotypes surface antigens evaluated. Their ability to form colony-forming units (CFUs) their potential...
The HBB gene encodes the β-globin protein, one of two main components adult hemoglobin A (HbA) responsible for oxygen transport. β-thalassemia is a genetic disorder caused by mutations affecting chain synthesis, leading to reduced or absent production, impaired erythropoiesis, and generally results in anemia. In this study, human-induced pluripotent stem cell line (hiPSC) MURAi006-A was generated from male fetal skin fibroblasts carrying both β⁰-thalassemia mutation at codon 17 (A > T) 26 (G...
The MUi019-A human induced pluripotent stem cell line was generated from peripheral blood CD34 + hematopoietic progenitors of a healthy woman using non-integrative reprogramming method. Episomal vectors carrying factors OCT4, SOX2, KLF4, L-MYC, LIN28, and shRNA TP53 EBNA-1 were delivered electroporation. iPSC can be used as control in studying disease mechanisms. Furthermore, gene editing approaches to introduce specific mutations into the model while type affected by is inaccessible.
Intracellular fatty acid‐binding proteins (FABPs) are small members of the superfamily lipid‐binding proteins, which occur in invertebrates and vertebrates. Included this cellular retinoic retinol‐binding seem to be restricted Here, we report cDNA cloning characterization two FABPs from hemocytes freshwater crayfish Pacifastacus leniusculus shrimp Penaeus monodon . In both these binding triad residues involved interaction with ligand carboxylate groups present. From sequence homology...
Human iPSC line MU011.A-hiPS was generated from homozygous α-thalassemia (-(SEA)/-(SEA)) fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 contained in three episomal vectors were delivered electroporation.
Reactivating of fetal hemoglobin (HbF; α2γ2) can ameliorate the severity β-thalassemia disease by compensating for adult deficiency in patients. Previously, microarray analysis revealed that zinc finger protein (ZNF)802 (also known as Juxta-posed with another gene-1 (JAZF1)) was upregulated human erythroblasts derived from peripheral blood compared liver-derived cells, implying a potential role HbF repressor. However, ZNF802 induced lentiviral shRNA β0-thalassemia/hemoglobinE had no effect...
A key event in human development is the establishment of erythropoietic progenitors bone marrow, which accompanied by a fetal-to-adult switch hemoglobin expression. Understanding this could lead to medical application, notably treatment sickle cell disease and β-thalassemia. The changes gene expression progenitor cells as they migrate from fetal liver colonize marrow are still rather poorly understood, primary (FL) tissues difficult obtain.We obtained FL tissue adult peripheral blood (AB)...
The Krüppel‑like factor (KLF) family dominates highly conserved three zinc finger DNA binding domains at the C‑terminus and variable transactivation N‑terminus. Humans possess 18 <em>KLF</em> genes that are differentially expressed in various tissues. Several KLFs recognize a specific CACCC motif is commonly found within hematopoietic‑specific promoters. To investigate those involved human hemoglobin (Hb) switching, present study analyzed previous microarray data set from fetal adult...
Ten-Eleven Translocation methylcytosine dioxygenase 1 (TET1) is known to play a broad tumor suppressor role through demethylating and activating genes. TET1 missense mutations are previously reported in many types of leukemia. Here, the human induced pluripotent stem cell line MURAi001-A was generated from skin fibroblasts derived 56-year-old female patient carrying gene mutation c.4404A > G (p.I1468M), who had history ovarian germ tumor. The demonstrated embryonic-like characteristics as it...
The human leukocyte antigen (HLA) system comprises cell-surface proteins responsible for the presentation of peptide antigens. HLAs play an essential role in regulation immune system, and their studies have been crucial to its understanding. To create a sustainable model investigation HLAs, we successfully generated iPSC line MURAi003-A derived from peripheral blood mononuclear cells donor with homozygous Class I II (A*11:01, B*46:01; C*01:02; DRB1*09:01; DQB1*03:03) using non-integrative...
The thalassemias are a group of genetic disorders characterized by deficiency in the synthesis globin chains. In this study MUi009-A human induced pluripotent stem cell line was successfully generated from peripheral blood CD34+ haematopoietic progenitors 32year old male who had coinherited homozygous β°-thalassemia mutation at codon 41/42 (-TCTT) and heterozygous α-thalassemia 4.2 deletion. exhibited embryonic characteristics with consistent pluripotency marker expression capability...
Mutations in MYH9 gene is one of the major causes inherited thrombocytopenia resulted from nonfunctional myosin-9 protein. We have generated a human induced pluripotent stem cell line MUi010-A skin fibroblasts patient who had point mutation c.2104C>T (p.R702C) exon 16 using non-integrative reprogramming method. The exhibited embryonic cell-like characteristics with consistent markers expression, was capable all three germ layers differentiation, and normal karyotype.
The 13q deletion syndrome is a rare chromosomal disorder caused by loss of the long arm chromosome 13, and usually entails developmental delay, intellectual disability, behavioral problems distinctive facial features. In this study, we successfully generated human iPSC line (MUi015-A) from skin fibroblasts patient who had large del(13)(q14q22). MUi015-A exhibited embryonic stem cell characteristics with consistent pluripotency marker expression capability differentiating into three germ...