Culture of cells as three-dimensional (3D) aggregates can enhance in vitro tests for basic biological research well therapeutics development. Such 3D culture models, however, are often more complicated, cumbersome, and expensive than two-dimensional (2D) cultures. This paper describes a 384-well format hanging drop plate that makes spheroid formation, culture, subsequent drug testing on the obtained cellular constructs straightforward to perform adapt existing high-throughput screening (HTS)...

We describe a microfabricated airway system integrated with computerized air-liquid two-phase microfluidics that enables on-chip engineering of human epithelia and precise reproduction physiologic or pathologic liquid plug flows found in the respiratory system. Using this device, we demonstrate cellular-level lung injury under flow conditions cause symptoms characteristic wide range pulmonary diseases. Specifically, propagation rupture plugs simulate surfactant-deficient reopening closed...

Triple-negative breast cancer (TNBC) is a poor prognostic with the highest mutations and limited therapeutic choices. Cytokine networking between cells tumor microenvironment (TME) maintains self-renewing subpopulation of stem (BCSCs) that mediate heterogeneity, resistance recurrence. Immunotherapy those factors combined targeted therapy or chemoagents may advantage TNBC treatment. We found oncogene Multiple Copies in T-cell Malignancy 1 (MCT-1/MCTS1) expression new poor-prognosis marker...

The ability to properly model intravascular steps in metastasis is essential identifying key physical, cellular, and molecular determinants that can be targeted therapeutically prevent metastatic disease. Research on the vascular microenvironment has been hindered by challenges studying this compartment under conditions reproduce vivo physiology while allowing facile experimental manipulation.We present a microfluidic vasculature system interactions between circulating breast cancer cells...

Abstract Three-dimensional (3D) tumor spheroid possesses great potential as an in vitro model to improve predictive capacity for pre-clinical drug testing. In this paper, we combine advantages of flow cytometry and microfluidics perform testing analysis on a large number (5000) uniform sized spheroids. The spheroids are formed, cultured treated with drugs inside microfluidic device. can then be harvested from the device without tedious operation. Due ample cell numbers, dissociated into...

Evaporation is a critical problem when handling submicroliter volumes of fluids. This paper characterizes this as it applies to microfluidic cell culture in poly(dimethylsiloxane) (PDMS) devices and provides practical solution. Evaporation-mediated osmolality shifts through PDMS membranes with varying thicknesses (10, 1, 0.2, or 0.1 mm) were measured over 96 h. Even humidified incubators, evaporation associated the media was significant prevented mouse embryo human endothelial growth...

Studies using this micro-system demonstrated significant morphological differences between alveolar epithelial cells (transformed human cell line, A549 and primary murine cells, AECs) exposed to combination of solid mechanical surface-tension stresses (cyclic propagation air–liquid interface wall stretch) compared populations solely cyclic stretch. We have also measured in both death detachment rates monolayers experiencing stresses. This research describes new tools for studying the...

This paper reports a microfluidic device capable of generating oxygen gradients for cell culture using spatially confined chemical reactions with minimal consumption. The is constructed by single-layer polydimethylsiloxane (PDMS) channels, in which the cells can be easily observed microscopes. control without utilization bulky pressurized gas cylinders, direct addition scavenging agents, or tedious interconnections and sophisticated flow control. In addition, due to efficient transportation...

This manuscript describes a straightforward fabrication process for embedding Ag/AgCl electrodes within two-layer poly(dimethylsiloxane) (PDMS) microfluidic chip where an upper and lower channel are separated by semiporous membrane. system allows the reliable real-time measurement of transendothelial transepithelial electrical resistance (TEER), accepted quantification cell monolayer integrity, across cells cultured on membranes inside microchannels using impedance spectroscopy. The...

This paper reports a PDMS–PC hybrid microfluidic device capable of performing cell culture under combinations chemical and oxygen gradients.

We previously reported the development of a simple, user-friendly, and versatile 384 hanging drop array plate for 3D spheroid culture importance utilizing cellular models in anti-cancer drug sensitivity testing. The allows high-throughput capabilities offers significant improvements over existing methods. To allow practical cell-based screening enable broader use plate, we characterize robustness terms assay performance demonstrate versatility plate. find that is robust fluorescence-...

Culture of cells as three-dimensional (3D) aggregates, named spheroids, possesses great potential to improve in vitro cell models for basic biomedical research. However, such spheroid are often complicated, cumbersome, and expensive compared conventional Petri-dish cultures. In this work, we developed a simple microfluidic device formation, culture, harvesting. Using device, could form uniformly sized spheroids due strong cell–cell interactions the spatial confinement culture chambers. We...

Abstract The small sized, flexible, high-performed and bio-compatible sensing devices are the critical elements to realize bio-related detection or on-site health monitoring systems. In this work, flexible localized surface plasmon resonance (LSPR) bio-sensors were demonstrated by integrating metal–insulator–metal (MIM) nanodisks with polydimethylsiloxane (PDMS) substrate. different geometries of MIM nanodisk sensors investigated optimized enhance spatial overlap LSPR waves environment,...

Oral cancer represents a global health burden, necessitating novel therapeutic strategies. Photodynamic and photothermal therapies using indocyanine green (ICG) have shown promise due to their distinctive near-infrared (NIR) light absorption characteristics FDA-approved safety profiles. This study develops ICG-loaded liposomes (Lipo-ICGs) further explore potential in oral treatments. We synthesized characterized the Lipo-ICGs, conducted vitro cell culture experiments assess cellular uptake...

We report fabrication and characterization of microfluidic devices made thermoplastic elastomeric polymers. These hard−soft hybrid material are motivated by the combined need for large scale manufacturability, enhanced barrier properties to gas permeation evaporation aqueous solutions compared poly(dimethyl siloxane) (PDMS) devices, compatibility with deformation-based actuation. Channel features created on rigid polymers such as polyethylene terephthalate glycol (PETG), cyclic olefin...

This paper reports a novel pressure sensor with an electrical readout based on electrofluidic circuits constructed by ionic liquid (IL)-filled microfluidic channels. The developed can be seamlessly fabricated into polydimethylsiloxane (PDMS) systems using the well-developed multilayer soft lithography (MSL) technique without additional assembly or sophisticated cleanroom microfabrication processes. Therefore, device easily scaled up and is fully disposable. sensing achieved measuring...

Various microfluidic cell culture devices have been developed for in vitro studies because of their capabilities to reconstitute vivo microenvironments. However, controlling flows is not straightforward due the wide varieties fluidic properties biological samples. Currently, flow observations mainly depend on optical imaging and macro scale transducers, which usually require sophisticated instrumentation are difficult up. Without real time monitoring, control can only rely theoretical...

Oxygen tension plays an important role in regulating various cellular functions both normal physiology and disease states. Therefore, drug testing using conventional vitro cell models under normoxia often possesses limited prediction capability. A traditional method of setting oxygen a liquid medium is by saturating it with gas mixture at the desired level oxygen, which requires bulky cylinders, sophisticated control, tedious interconnections. Moreover, only single can be tested same time....