A5012305105- Plant Reproductive Biology
- Plant and animal studies
- Transgenic Plants and Applications
- Botanical Research and Chemistry
- Plant Molecular Biology Research
Pennsylvania State University
2017-2018
Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains pistil-specific S-RNase gene and multiple pollen-specific F-box (SLF) genes. Both gain-of-function loss-of-function experiments have shown that alone regulates pistil specificity SI. Gain-of-function on SLF genes suggest entire suite of encoded proteins constitute pollen determinant. However, clear-cut must be performed to determine if are essential for SI pollen. Here, we...
Petunia inflata possesses a self-incompatibility (SI) mechanism, which involves S-RNase and multiple S-locus F-box (SLF) genes at the polymorphic S-locus. For given S-haplotype, each SLF is thought to interact with some of its non-self S-RNases, but not self S-RNase. In this work, we studied an allelic pair SLF1, S2-SLF1 S3-SLF1, differ in 44 amino acids show differential interactions S3-RNase. We first used vivo transgenic assay determine whether four chimeric proteins one three functional...