A5100404339- Molecular Biology Techniques and Applications
- Muscle Physiology and Disorders
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Protein Interaction Studies and Fluorescence Analysis
- Plant and animal studies
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Angiogenesis and VEGF in Cancer
- dental development and anomalies
- Photosynthetic Processes and Mechanisms
- Histone Deacetylase Inhibitors Research
- Drug Transport and Resistance Mechanisms
- Ubiquitin and proteasome pathways
- Animal Genetics and Reproduction
- bioluminescence and chemiluminescence research
- Water Treatment and Disinfection
- Protein Degradation and Inhibitors
- Photochemistry and Electron Transfer Studies
- Galectins and Cancer Biology
- Plant Diversity and Evolution
- Biotin and Related Studies
- Chromatin Remodeling and Cancer
- Peptidase Inhibition and Analysis
Affiliated Hospital of Youjiang Medical University for Nationalities
2022-2024
State Key Laboratory of Biotherapy
2023
Sichuan University
2023
Pennsylvania State University
2013-2018
Guangxi Institute of Botany
2017
Army Medical University
2016
Institute of Process Engineering
2015
Tianjin University
2015
National Institute for Radiological Protection
2015
Capital Medical University
2015
Graft-copolymers, containing poly(ethylene glycol) (PEG) and polyethyleneimine (PEI) chains have been proposed as carriers for delivery of phosphorothioate oligonucleotides (SODNs). Complexes such copolymers with SODN self-assemble into particles having a core neutralized PEI corona PEG. Transferrin molecules are attached to the PEG using avidin/biotin construct. For this purpose, biotin moieties covalently linked free ends in PEG-g-PEI copolymer. SODNs reacted mixtures biotinylated...
S-RNase-based self-incompatibility in Petunia is a self/non-self recognition system that allows the pistil to reject self-pollen prevent inbreeding and accept non-self pollen for outcrossing. Cloning of S-RNase 1986 marked beginning nearly three decades intensive research into mechanism this complex system. was shown be sole female determinant 1994, first male determinant, S-locus F-box protein1 (SLF1), identified 2004. It discovered 2010 additional SLF proteins are involved specificity,...
Self-incompatibility (SI) in Petunia is regulated by a polymorphic S-locus. For each S-haplotype, the S-locus contains pistil-specific S-RNase gene and multiple pollen-specific F-box (SLF) genes. Both gain-of-function loss-of-function experiments have shown that alone regulates pistil specificity SI. Gain-of-function on SLF genes suggest entire suite of encoded proteins constitute pollen determinant. However, clear-cut must be performed to determine if are essential for SI pollen. Here, we...
Corona Virus Disease 2019 (COVID-19) has brought the new challenges to scientific research. <em>Isodon suzhouensis</em> good anti-inflammatory and antioxidant stress effects, which is considered as a potential treatment for COVID-19. The possibility of COVID-19 with <em>suzhouensis </em>and its mechanism action were explored by employing molecular docking network pharmacology. Network pharmacology used screen drug targets, Lipopolysaccharide (LPS) induced RAW264.7 NR8383 cells inflammation...
ABSTRACT Anthocyanin is one of the flavonoid phytopigments with specific health benefits. The interaction between delphinidin‐3‐ O ‐glucoside (D3G) and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy, synchronous three‐dimensional ultraviolet‐visible absorption circular dichroism spectroscopy molecular modeling. D3G effectively quenched intrinsic BSA via static quenching. number binding sites constant K a were determined, hydrogen bonds van der Waals forces played...
Summary Many flowering plants show self‐incompatibility, an intra‐specific reproductive barrier by which pistils reject self‐pollen to prevent inbreeding and accept non‐self pollen promote out‐crossing. In Petunia , the polymorphic S–locus determines self/non‐self recognition. The locus contains a gene encoding S– RN ase, controls pistil specificity, multiple S‐locus F‐box ( SLF ) genes that collectively control specificity. Each is component of SCF (Skp1/Cullin/F‐box) complex responsible...
The collaborative non-self-recognition model for S-RNase-based self-incompatibility predicts that multiple S-locus F-box proteins (SLFs) produced by pollen of a given S-haplotype collectively mediate ubiquitination and degradation all non-self S-RNases, but not self in the tube, thereby resulting cross-compatible pollination self-incompatible pollination. We had previously used extracts containing GFP-fused S2 -SLF1 (SLF1 with an -haplotype) Petunia inflata co-immunoprecipitation (Co-IP)...
As a third-generation sequencing (TGS) method, single-molecule real-time (SMRT) technology provides long read length, and it is well suited for resequencing projects de novo assembly. In the present study, Pseudomonas aeruginosa PA1 was characterized resequenced using SMRT technology. also subjected to genomic, comparative pan-genomic analyses. The multidrug resistant strain possesses 6,498,072 bp genome sequence type of ST-782. visualized, results revealed details general annotations,...
Gap junction (GJ) coupling is involved in the regulation of spontaneous Ca(2+) transients neonatal rat ventricular myocytes (NRVMs); however, underlying mechanism(s) remains incompletely clear. In this study, we investigated Na influx mediated by Cx43-associated GJ modulating NRVM excitation. Intracellular Na(+) and concentrations ([Na(+)](i) [Ca(2+)](i)) were assessed using CoroNa Green Fluo-4 fluorescent probes, respectively, confocal microscopy. function was evaluated measuring...
Primulina wenii (Gesneriaceae) is described and illustrated here as a new species. Morphologically, this species resembles P. juliae (Hance) Mich. Möller & A. Weber, but it distinct from the latter by some obvious features in leaf blade, bract, indumentum, corolla, filament, stigma pistil. Nuclear ribosomal internal transcribed spacer (ITS) region plastid trnL-F intronspacer (trnL-F) DNA sequence data its 22 relatives are used to determine systematic position of within Primulina....