A5015599313- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Biosensors and Analytical Detection
- Plant Pathogenic Bacteria Studies
- Bacterial Identification and Susceptibility Testing
- Connexins and lens biology
- Streptococcal Infections and Treatments
- Plant Virus Research Studies
- Advanced biosensing and bioanalysis techniques
- Oral microbiology and periodontitis research
- Quantum optics and atomic interactions
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 detection and testing
- Infections and bacterial resistance
- Biochemical Analysis and Sensing Techniques
- Atomic and Subatomic Physics Research
- Heat shock proteins research
- Genomics and Chromatin Dynamics
- Aldose Reductase and Taurine
Army Medical University
2015-2024
Army Medical College
2023
Nankai University
2012-2020
Chongqing University
2018
Chinese Culture University
1995
National Taiwan University
1995
Academia Sinica
1995
New agents with particular specificity toward targeted bacteria and superefficacy in antibacterial activity are urgently needed facing the crisis of worldwide antibiotic resistance. Herein, a novel strategy by equipping bacteriophage (PAP) photodynamic inactivation (PDI)-active AIEgens (luminogens aggregation-induced emission property) was presented to generate type AIE–PAP bioconjugate superior capability for both imaging synergistic killing certain species bacteria. The targeting ability...
Capsaicin reduces body weight mainly through activation of transient receptor potential vanilloid 1 (TRPV1) cation channel. However, recent evidence indicates that the gut microbiota influences many physiological processes in host and might provoke obesity. This study determined whether anti-obesity effect capsaicin is related to changes microbiota. C57BL/6 mice were fed either with high-fat diet (HFD) or HFD (HFD-CAP) for nine weeks. We observed a significantly reduced gain improved glucose...
Bacteria develop a broad range of phage resistance mechanisms, such as prevention adsorption and CRISPR/Cas system, to survive predation. In this study, Pseudomonas aeruginosa PA1 strain was infected with lytic PaP1 phage-resistant mutants were selected. A high percentage (~30%) these displayed red pigmentation phenotype (Red mutant). Through comparative genomic analysis, one Red mutant PA1r found have 219.6 kb fragment deletion, which contains two key genes hmgA galU related the observed...
We isolated and characterized a new Pseudomonas aeruginosa myovirus named PaP1. The morphology of this phage was visualized by electron microscopy its genome sequence ends were determined. Finally, genomic proteomic analyses performed. PaP1 has an icosahedral head with apex diameter 68–70 nm contractile tail length 138–140 nm. is linear dsDNA molecule containing 91,715 base pairs (bp) G+C content 49.36% 12 tRNA genes. A strategy to identify the designed. 1190 bp terminal redundancy. 157 open...
As a potential antibacterial agent, endolysin can directly lyse Gram-positive bacteria from the outside and does not lead to drug resistance. Considering that XN108 is first reported methicillin-resistant Staphylococcus aureus (MRSA) strain in mainland China with vancomycin MIC exceeds 8 µg mL -1 , we conducted systematic study on its phage-encoded LysP108. Standard plate counting method revealed LysP108 could S. Pseudomonas aeruginosa damaged outer membrane, resulting significant reduction...
Abstract Background Staphylococcus aureus and its single or mixed biofilm infections seriously threaten global public health. Phage therapy, which uses active phage particles phage-derived endolysins, has emerged as a promising alternative strategy to antibiotic treatment. However, high-efficient therapeutic regimens have yet be established. Results In this study, we used an enrichment procedure isolate phages against methicillin-resistant S. (MRSA) XN108. We characterized SYL, new member of...
Pseudomonas aeruginosa is an opportunistic pathogen with a relatively large genome, and has been shown to routinely lose genomic fragments during environmental selection. However, the underlying molecular mechanisms that promote chromosomal deletion are still poorly understood. In recent study, we showed by deleting fragment containing two closely situated genes, hmgA galU, P. was able form 'brown mutants', bacteriophage (phage) resistant mutants brown color phenotype. this show occur at...
Abstract The interactions between Bacteriophage (phage) and host bacteria are widespread in nature influences of phage replication on the cells complex extensive. Here, we investigate genome-wide Pseudomonas aeruginosa ( P. aeruginosa) its temperate PaP3 at five time points during infection. Compared to uninfected host, 38% (2160/5633) genes phage-infected were identified as differentially expressed (DEGs). Functional analysis repressed DEGs revealed infection-stage-dependent pathway...
Bacteriophages (phages) are widely distributed in the biosphere and play a key role modulating microbial ecology soil, ocean, humans. Although of DNA bacteriophages is well described, biology RNA poorly understood. More than 1900 phage genomes currently deposited NCBI, but only 6 dsRNA 12 ssRNA genome sequences reported. The were isolated from legume samples or lakes with Pseudomonas syringae as host. Here, we report first aeruginosa phiYY three-segmented genome. was hospital sewage China...
Toxin-antitoxin (TA) systems are small genetic modules that widely distributed in the genomes of bacteria and archaea have been proposed to fulfill numerous functions. Here, we describe identification characterization a type II TA system, comprising hicAB locus human opportunistic pathogen Pseudomonas aeruginosa. The consists genes hicA hicB encoding toxin its cognate antitoxin, respectively. BLAST analysis revealed is prevalent approximately 36% P. aeruginosa strains locates same genomic...
A virulent bacteriophage highly specific to Pseudomonas aeruginosa (P. aeruginosa) was isolated from hospital sewage using a lambda isolation protocol. The bacteriophage, named as PAP1, used functionalize tosyl-activated magnetic beads establish bacteriophage-affinity strategy for separation and detection of viable P. aeruginosa. Recognition the target bacteria by tail fibers baseplate led capture onto beads. After replication cycle about 100 min, progenies lysed released intracellular...
Rapid and accurate bacterial detection is crucial to an early diagnosis for treating various infectious diseases. A recombinant tail fiber protein (P069) of the Pseudomonas aeruginosa (P. aeruginosa) phage was expressed in Escherichia coli. After renaturation at a low temperature, inclusion body P069 successfully transformed aqueous soluble that retained capacity recognizing P. aeruginosa. The did not show lytic activity aeruginosa, which facilitated capture manipulation whole cells with...
Abstract Staphylococcus aureus ( S. ), especially methicillin‐resistant (MRSA), causes wound infections, whose treatment remains a clinical challenge. Bacterium‐infected wounds often create acidic niches with pH 4.5–6.5. Endolysin LysSYL, which is derived from phage SYL, shows promise as an antistaphylococcal agent. However, endolysins generally exhibit instability and possess low bioavailability in microenvironments. Here, array of self‐assembling peptides designed, peptide L5 screened out...
Streptococcus suis has emerged as a causative agent of human meningitis and streptococcal toxic shock syndrome over the last years. The high pathogenicity S. may be due in part to laterally acquired island (renamed SsPI-1), which can spontaneously excise transfer recipients. Cells harboring excised SsPI-1 potentially lose this if cell division occurs prior its reintegration; however, attempts cure from host cells have been unsuccessful. Here, we report that an SsPI-1-borne Epsilon/Zeta...
Methicillin-resistant Staphylococcus aureus (MRSA) has been well-recognized as one of the most common multiresistant bacteria threatening human health. Broad-spectrum recognition multiple MRSA strains can meet urgent demands for efficient diagnosis and subsequent decision relevant treatment MRSA-induced infections. Here, recombinant cell-binding domain (CBD) green fluorescent protein-fused CBD bacteriophage were expressed in soluble form. Distinct from strain-specific bacteriophage, both...
As a multidrug-resistant pathogen, Acinetobacter baumannii has long been identified as one of the most common nosocomial bacteria. High-performance recognition probes for wide-spectrum detection A. are highly desired to achieve efficient diagnosis and timely treatment infectious diseases induced by this pathogen. An engineering tail fiber protein (ETFP) named Gp50 encoded lytic phage Abp9 was expressed in Escherichia coli binding baumannii. According results genome sequencing an wild strain...
Adaptation of bacteria to phage predation poses a major obstacle for therapy. Bacteria adopt multiple mechanisms, such as inhibition adsorption and CRISPR/Cas system, resist infection. Here, phage-resistant mutant Pseudomonas aeruginosa strain PA1 under the infection lytic PaP1 was selected further study. The PaP1-resistant variant, termed PA1RG, showed decreased devoid long chain O-antigen on its cell envelope. Whole genome sequencing comparative analysis revealed single nucleotide mutation...
As a potential alternative to antibiotics, phages can be used treat multi-drug resistant bacteria. such, the biological characteristics of should investigated utilize them as effective antimicrobial agents. In this study, phage PaoP5, lytic virus that infects Pseudomonas aeruginosa PAO1, was isolated and genomically characterized. PaoP5 comprises an icosahedral head with apex diameter 69 nm contractile tail length 120 nm. The genome is linear dsDNA molecule containing 93,464 base pairs (bp)...
The pathogen of plague is Yersinia pestis (Y. pestis), one the deadliest pathogens in world and belonging to family Enterobacteriaceae. In this work, biological characteristics complete genome sequence analysis a novel lytic Y. pestis-specific phage JC221 isolated from Yunnan Province, China, was studied. belongs Myoviridae has regular icosahedral head long contractile tail. double-stranded DNA contains 174,931 bp, G + C content 41.23 %. There are 274 predicted genes, which only 103 hits...
The study of carrier state phages challenged the canonical lytic-lysogenic binary, and appears to be ubiquitous ecologically important. However, mechanisms are not well elucidated due limited phage models. Herein, we reported HQ103, similar Escherichia coli P2. In contrast temperate P2 phage, HQ103 does insert its genome into bacterial chromosome displays a dual behavior depending on temperature. At 37°C, lyses host forms clear plaques truncation repressor CI mutation promoter Pc. contrast,...