A5022189473- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- CAR-T cell therapy research
- Antimicrobial Peptides and Activities
- T-cell and B-cell Immunology
- COVID-19 Clinical Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Innovative Teaching and Learning Methods
- Long-Term Effects of COVID-19
- SARS-CoV-2 detection and testing
- Intelligent Tutoring Systems and Adaptive Learning
- Youth Development and Social Support
- interferon and immune responses
- Advanced Biosensing Techniques and Applications
- Visual and Cognitive Learning Processes
- RNA Interference and Gene Delivery
- Early Childhood Education and Development
- Grit, Self-Efficacy, and Motivation
University of Tübingen
2016-2022
McMaster University
1999
Background Peptide-based vaccination is a rational option for immunotherapy of prostate cancer. In this first-in-man phase I/II study, we assessed the safety, tolerability and immunological impact synthetic long peptide vaccine targeting Ras homolog gene family member C (RhoC) in patients with RhoC small GTPase overexpressed advanced solid cancers, metastases cancer stem cells. Methods Twenty-two who had previously undergone radical prostatectomy received subcutaneous injections 0.1 mg...
The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution distinct immune cell populations. Considering key role CD4 + cells in regulating immunological processes, we generated novel single-domain antibodies [nanobodies (Nbs)] that specifically recognize human CD4. After in-depth analysis their binding properties, recognized epitopes, effects on T-cell proliferation, activation, cytokine release, selected CD4-specific Nbs did not...
We investigated blood samples from fully SARS-CoV2-vaccinated subjects and previously positive tested patients up to one year after infection with SARS-CoV2, compared short- long-term T cell antibody responses, a special focus on the recently emerged delta variant (B.1.617.2).In 23 vaccinated subjects, we documented high anti-SARS-CoV2 spike protein receptor binding domain (RBD) titers. Average virus neutralization by antibodies, assessed as inhibition of ACE2 RBD, was 2.2-fold reduced for...
Abstract The challenging human pathogen Staphylococcus aureus has highly efficient immune evasion strategies for causing a wide range of diseases, from skin and soft tissue to life-threatening infections. Phenol-soluble modulin (PSM) peptides are major pathogenicity factors community-associated methicillin-resistant S. strains. In previous work, we demonstrated that PSMs in combination with TLR2 ligand induce tolerogenic dendritic cells (DCs) characterized by the production high amounts...
Elongated peptides (EPs), containing possibly one or multiple epitope/s, are increasingly used for the screening of antigen-specific CD8+ and CD4+ cell responses. Here, we present an in vitro protocol that allows amplification cells subsequent functional analysis both T types using EPs. Known viral-derived epitopes were elongated to 20 mer EPs on N-, C-, termini HLA class I binders, N- C- II binders. With EP stimulation only, percentage responding was dependent elongation site EP, whereas...
Staphylococcus aureus (Sa), as one of the major human pathogens, has very effective strategies to subvert immune system. Virulence emerging community-associated methicillin-resistant Sa (CA-MRSA) depends on secretion phenol-soluble modulin (PSM) peptide toxins e.g. by binding and modulation innate cells. Previously, using mouse bone marrow-derived dendritic cells we demonstrated that PSMs in combination with various Toll-like receptor (TLR) ligands induce a tolerogenic DC phenotype (tDC)...
Dendritic cells (DCs) are key players of the immune system and thus a target for evasion by pathogens. We recently showed that virulence factors phenol-soluble-modulins (PSMs) produced community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strains induce tolerogenic DCs upon Toll-like receptor activation via p38-CREB-IL-10 pathway in vitro. Here, we addressed hypothesis S. PSMs disturb adaptive response modulation DC subsets vivo. Using systemic mouse infection model...
Abstract Objective We investigated blood samples from fully SARS-CoV2-vaccinated subjects and previously positive tested patients up to one year after infection with SARS-CoV2, compared short- long-term T cell antibody responses, a special focus on the recently emerged delta variant (B.1.617.2). Methods Results In 23 vaccinated subjects, we documented high anti-SARS-CoV2 spike protein receptor binding domain (RBD) titers. Average virus neutralization by antibodies, assessed as inhibition of...
Abstract The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution distinct immune cell populations. Considering key role CD4 + T cells in regulating immunological processes, we generated novel single-domain antibodies (nanobodies, Nbs) that specifically recognize human CD4. After depth analysis their binding properties, recognized epitopes, effects on proliferation, activation cytokine release, selected Nbs did not interfere with crucial...