A5000877677- Chronic Lymphocytic Leukemia Research
- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- Lymphoma Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Immunodeficiency and Autoimmune Disorders
- Chronic Myeloid Leukemia Treatments
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Cancer therapeutics and mechanisms
- Platelet Disorders and Treatments
- Drug Transport and Resistance Mechanisms
- Single-cell and spatial transcriptomics
- Acute Lymphoblastic Leukemia research
- Testicular diseases and treatments
- Cell Adhesion Molecules Research
- Synthesis and bioactivity of alkaloids
- Ion Channels and Receptors
- Bioactive Compounds and Antitumor Agents
- Sarcoma Diagnosis and Treatment
- Cystic Fibrosis Research Advances
- Cholesterol and Lipid Metabolism
- Calcium signaling and nucleotide metabolism
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Inflammatory Myopathies and Dermatomyositis
Institut Bergonié
2010-2023
Hôpital Cardiologique du Haut-Lévêque
1994-2000
University of Edinburgh
1998-2000
Centre Hospitalier Universitaire de Bordeaux
2000
Osaka University
1998
Mayo Clinic
1998
Université de Bordeaux
1993
Background Procaspase 3 is a constitutive proenzyme that activated by cleavage during apoptosis. The resulting enzyme able to cleave several target proteins after the second aspartate of DEVD sequence common all substrates caspases and 7 (DEVDase). Because active caspase effector in apoptotic pathways, it may be good marker detect (pre-)apoptotic cells flow cytometry (FCM). Materials Methods Apoptosis was induced U937 or bone marrow mononuclear daunorubicin (DNR), idarubicin (IDA),...
We have compared cytoplasmic extracts from chicken DU249 cells at various stages along the apoptotic pathway. Extracts morphologically normal “committed stage” induce morphology and DNA cleavage in substrate nuclei but require ongoing caspase activity to do so. In contrast, frankly events added a caspase-independent manner. Biochemical fractionation of these reveals that column fraction enriched endogenous active caspases is unable fragmentation or chromatin condensation nuclei, whereas...
Previous studies have demonstrated that topoisomerase I is cleaved late during apoptosis, but not identified the proteases responsible or examined functional consequences of this cleavage. Here, we shown treatment purified with caspase-3 resulted in cleavage at DDVD146 downward arrowY and EEED170 arrowG, whereas caspase-6 PEDD123 arrowG arrowG. After Jurkat T lymphocytic leukemia cells anti-Fas antibody A549 lung cancer topotecan, etoposide, paclitaxel, fragment comigrated product from...
Abstract Background: The development of multiparameter flow cytometry (FCM) and increasingly sophisticated analysis software has considerably improved the exploration hematological disorders. These tools have been widely applied in leukaemias, lymphomas, myelodysplasias, yet with very heterogeneous approaches. Consequently, there is no extensive reference document reporting on characteristics normal human bone marrow (BM) FCM. Here, we report a procedure using relevant antibody combinations...
Flow cytometry allows specific assessment of the expression ZAP-70, a promising new prognostic factor in B-cell chronic lymphocytic leukemia (B-CLL), but suffers from lack multicenter standardization.An optimized method for direct detection ZAP-70 flow was tested fashion. Adapted frozen cells, this includes normalization step by addition B cells pool peripheral blood mononuclear collected normal donors. levels were assessed 153 patients with typical leukemia. Results expressed as ratio mean...
GA101/obinutuzumab is a novel type II anti-CD20 monoclonal antibody (mAb), which more effective than rituximab (RTX) in preclinical and clinical studies when used combination with chemotherapy. Ca2+ signaling was shown to play role RTX-induced cell death. This report concerns the effect of GA101 on its involvement direct death induced by GA101. We reveal that triggered an intracellular increase mobilizing stores activating Orai1-dependent influx non-Hodgkin lymphoma lines primary B-Cell...
Abstract Background: Flow cytometry is the sole available technique for quantification of tumor plasma‐cells in plasma‐cell disorders, but so far, no consensus has been proposed. Here, we report on a standardized, simple, robust five color flow protocol developed to characterize and quantify bone marrow plasma‐cells, validated multicenter manner. Methods: CD36 was used exclude red blood cell debris erythroblasts, CD38 CD138 detect immunoglobulin light chains, CD45, CD56, CD19, CD117 + CD34...
Abstract Purpose To assess the incidence of BCR‐ABL kinase domain (KD) mutation detection and its prognostic significance in chronic phase myeloid leukemia (CP‐CML) patients treated with tyrosine inhibitors (TKIs). Patients Methods We analyzed characteristics outcome 253 CP‐CML who had at least one analysis performed using direct sequencing. Of them, 187 were early CP (ECP) 66 late (LCP) 88% Imatinib as first‐line TKI. Results Overall, 80 (32%) harbored KD mutations. A was identified 57%...
Eradication of minimal residual disease (MRD), at the end Fludarabine-Cyclophosphamide-Rituximab (FCR) treatment, is a validated surrogate marker for progression-free and overall survival in chronic lymphocytic leukaemia. But such deep responses are also associated with severe immuno-depletion, leading to infections development secondary cancers. We assessed, blood MRD normal immune cell levels 162 first-line FCR patients, analysed adverse event. Multivariate Landmark analysis 3 months after...
Background: Tyrosine Kinase Inhibitors (TKIs) discontinuation in patients who had achieved a deep molecular response (DMR) offer now the opportunity of prolonged treatment-free remission (TFR). Patients and Methods: Aims this study were to evaluate proportion de novo chronic-phase chronic myeloid leukemia (CP-CML) sustained DMR identify predictive factors recurrence-free survival (MRFS) after TKI discontinuation. Results: Over period 10 years, 398 CP-CML treated with first-line TKIs...
Summary The diagnosis of Waldenström Macroglobulinaemia ( WM )/lymphoplasmacytic lymphoma LPL ) remains one exclusion because other B‐cell lymphoproliferative disorders (B‐ LPD ), such as marginal zone MZL can fulfil similar criteria, including MYD 88 L265P mutation. It has been suggested that expression the myeloid marker CD 13 (also termed ANPEP is more frequent in than B‐ and also described on normal malignant plasma cells. Here, was tested a cohort 1037 patients from 3 centres by flow...
The development of flow cytometry as a useful tool for the detection minimal residual disease (MRD) in chronic lymphocytic leukemia (CLL) is potentially hampered by fact that normal subset B-cells with similar immunophenotype present peripheral blood. This CLL-like cells not well defined terms frequency.Here, we performed multicenter study panel four-color antibody combinations possibly MRD CLL, to establish levels 49 healthy controls. ROC curves established upper level such at 4 × 10(-4) ....
Background As part of quality assurance in laboratories (labs) offering clinical cell analysis by flow cytometry (FCM), counting precision and robustness are evaluated but international desirable ranges still missing. The aim this study was to provide interlaboratory variability reference values. Methods A prospective survey on monthly assessment proposed all French routinely performing lymphocyte subpopulation quantification, over one arbitrarily selected month (June 2017), regardless...
Background: Bone marrow is a very unusual site of metastasis for germ cell tumors. Case Report: We report the case 21-year-old male patient who was treated with chemotherapy and secondary surgery primary mediastinal non-seminomatous tumor (NSGCT). The achieved complete remission. However, 4 months after completion therapy, he complained rapidly worsening bone pain. No evidence disease relapse found in computed tomography scan thorax abdomen, magnetic resonance imaging spine, or scan. A blood...
Some forms of chemoresistance in leukemia may start from failure tumour cells to successfully undergo apoptosis and Bcl-2 play a role this defect. Therefore, we evaluated the content synthesis relation with apoptotic potential leukemic cell lines after anthracycline treatment.U937, HL60, K562 their drug resistant (DR) variants were treated varying concentrations Idarubicin (IDA). Apoptosis was by fluorescence microscopy acridine orange staining. Bax either flow cytometry indirect...