A5054004945- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Caveolin-1 and cellular processes
- Lipid Membrane Structure and Behavior
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
- Glycosylation and Glycoproteins Research
- Neurogenetic and Muscular Disorders Research
- Cancer, Lipids, and Metabolism
- Ion channel regulation and function
- RNA modifications and cancer
- Platelet Disorders and Treatments
- Mesenchymal stem cell research
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Peroxisome Proliferator-Activated Receptors
- Cancer-related gene regulation
- Lipid metabolism and biosynthesis
- Digital Imaging for Blood Diseases
- Phagocytosis and Immune Regulation
- Polysaccharides and Plant Cell Walls
- Nuclear Structure and Function
- RNA Interference and Gene Delivery
- Metabolomics and Mass Spectrometry Studies
- Apelin-related biomedical research
Emory University
2012-2024
Children's Healthcare of Atlanta
2012-2017
Medical College of Wisconsin
2017
Versiti Blood Center of Wisconsin
2017
Korea University
2004-2014
Ministry of Health and Welfare
2005
Ministry of Science ICT and Future Planning
2005
The lipid-rafts hypothesis proposes that naturally occurring lipid aggregates exist in the plane of membrane are involved signal transduction, protein sorting, and transport. To understand their roles cell biology, a direct visualization such domains living cells is essential. For this purpose, 6-dodecanoyl-2-(dimethylamino)naphthalene (laurdan), probe sensitive to polarity membrane, has often been used. We have synthesized characterized...
Since detergent-resistant lipid rafts are involved in pathogen invasion, cholesterol homeostasis, angiogenesis, neurodegenerative diseases and signal transduction, protein identification the could provide important information to study their function. Here, we analyzed raft proteins isolated from rat liver by capillary liquid chromatography-tandem mass spectrometry. Out of 196 identified, 32% belonged or plasma membrane, 24% mitochondrial, 20% microsomal, 7% miscellaneous, 17% unknown...
Mitochondrial biogenesis is known to accompany adipogenesis complement ATP and acetyl-CoA required for lipogenesis. Here, we demonstrated that mitochondrial proteins such as synthase α β, cytochrome c were highly expressed during the 3T3-L1 differentiation into adipocytes. Fully-differentiated adipocytes showed a significant increase of mitochondria under electron microscopy. Analysis by immunofluorescence, cellular fractionation, surface biotinylation elevated levels complex found not only...
To study the interactions of cytoplasmic calcium elevation, mitochondrial permeability transition pore (mPTP) formation, and reactive oxygen species formation in regulation phosphatidylserine (PS) exposure platelets.mPTP but not degree was associated with PS wild-type, cyclophilin D-null, ionomycin-treated, species-treated platelets. In absence mPTP regulator D, agonist-initiated high-level were markedly blunted, transients unchanged. Mitochondrial (Ca(2+)(mit)) species, key regulators...
Platelets express a variety of membrane and secreted glycoproteins, but the importance glycosylation to platelet functions is poorly understood. To explore O-glycosylation, we generated mice with targeted deletion Cosmc in murine endothelial/hematopoietic cells (EHC) (EHC −/ y ). X-linked encodes an essential chaperone that regulates protein O-glycosylation. This mutation resulted lethal perinatal hemorrhage majority mice, surviving displayed severely prolonged tail-bleeding times...
Skeletal muscle is comprised of a heterogeneous population fibers which can be classified by their metabolic and contractile properties (fiber "types"). Fiber type primary determinant function along with fiber size (cross-sectional area). The composition responds to physiological changes like exercise aging often altered in disease states. Thus, analysis histological preparations useful method for quantifying key indicators measuring responses variety stimuli or stressors. These analyses are...
In order to detect and identify ubiquitous lipid raft marker proteins, we isolated rafts from different mouse organs, including the liver, lung, large brain, kidney, analyzed their proteins via 2-DE. Many protein spots were determined be in all of rafts, annotated LC MS/MS. Twelve identified as most these mitochondrial mortalin, prohibitin, voltage-dependent anion channel, ATP synthase, NADH dehydrogenase, ubiquinol-cytochrome c reductase. Via immunoblotting, shown exist detergent-resistant...
Phosphatidylserine exposure mediates platelet procoagulant function and regulates life span. Apoptotic, necrotic, integrin-mediated mechanisms have been implicated as intracellular determinants of phosphatidylserine exposure. Here, we investigate (1) the role mitochondrial events in initiated by these distinct stimuli (2) cellular interactions vitro vivo.Key were examined, including cytochrome c release inner membrane (IMM) disruption. In both ABT-737 (apoptotic) agonist (necrotic)-treated...
Abstract Since insulin receptors and their downstream signaling molecules are organized in lipid rafts, proteomic analysis of adipocyte rafts may provide new insights into the function adipogenesis signaling. To search for proteins involved differentiation signaling, we analyzed detergent‐resistant raft from 3T3‐L1 preadipocytes adipocytes by 2‐DE. Eleven were identified adipocytes. One adipocyte‐specific was globular C1q receptor (gC1qR), an acidic 32 kDa protein known as domain complement...
The pharyngeal muscles of the nasal, oral, and laryngeal pharynxes are required for swallowing. Pharyngeal preferentially affected in some muscular dystrophies yet spared others. Muscle stem cells, called satellite may be critical factors development muscle disorders; however, very little is known about cells (PSC) their role muscles. We show that PSC distinct from commonly studied hindlimb both transcriptionally biologically. Under basal conditions proliferate, progress through myogenesis,...
Cell-cell adhesion molecules play key roles in maintaining quiescence or promoting activation of various stem cells their niche. Muscle called satellite (SC) are critical for skeletal muscle regeneration after injury, but little is known about the role regulating behavior these cells. Vascular cell molecule-1 (VCAM-1) a cell-cell protein expressed on quiescent and activated SC whose function unknown this context. We deleted Vcam1 from using an inducible Cre recombinase young mice. In injured...
To address whether mitochondrial biogenesis is essential for skeletal myogenesis, C2C12 myogenesis was investigated after knockdown of NADH dehydrogenase (ubiquintone) flavoprotein 1 (NDUFV1), which an oxidative phosphorylation complex I subunit that the first to accept electrons from NADH. The NDUFVI enhanced by decreasing NAD(+)/NADH ratio and subsequently inactivating SIRT1 activators (pyruvate, SRT1720, resveratrol) abolished NDUFV1 knockdown-induced enhancement. However,...
Two-photon fluorescent probes for the cellular membrane, derived from 6-acyl-2-aminonaphthalene as fluorophore and hexanoyl (CH), lauryl (CL), stearyl (CS) groups receptor, have been synthesized. Their photophysical properties utility membrane were also studied. Whereas CH cannot be used a probe due to its high water solubility, CL CS are useful two-photon lateral heterogeneity, they can easily stain cells, emit fluorescence with sensitivity environment polarity, capable of imaging...
Satellite cells are stem with an essential role in skeletal muscle repair. Precise regulation of gene expression is critical for proper satellite cell quiescence, proliferation, differentiation and self-renewal. Nuclear proteins required dependent on the nucleocytoplasmic transport machinery to access nucleus, however little known about nuclear cells. The best characterized import pathway classical which depends a localization signal (cNLS) cargo protein heterodimeric receptors, karyopherin...
Abstract Different proteins are found in lipid rafts depending on the isolation method. For example, insulin receptor was predominantly raft fractions prepared from HepG2 cells with Brij 35, but were not present isolated Triton X‐100. In order to assess effect of detergent type and temperature isolation, analyzed by two‐dimensional (2‐D) electrophoresis. More protein spots appeared when 35 than addition, more at 37°C 4°C, indicating that much stable physiological (37°C) presence detergents....
Extracellular ATP (exATP) has been known to be a critical ligand regulating skeletal muscle differentiation and contractibility. ExATP synthesis was greatly increased with the high level of adenylate kinase 1 (AK1) synthase β during C2C12 myogenesis. The exATP abolished by knock-down AK1 but not that in myotubes, suggesting is required for myotubes. However, membrane-bound AK1β involved because its expression decreased myogenesis spite localization lipid rafts contain various kinds receptors...
Since detergent-resistant lipid rafts play important roles in the signal transduction for myogenesis, their comprehensive proteomic analysis could provide new insights to understand function myotubes. Here, were isolated from C2C12 myotubes and analyzed by capillary RPLC/MS/MS. Among 327 proteins (or protein groups) identified, 28% categorized plasma membrane or raft proteins, 29% mitochondria, 20% microsomal 10% other 13% unknown proteins. The localization of oxidative phosphorylation...
Previous reports raised question as to whether 8-chloro-cyclic adenosine 3,5-monophosphate (8-Cl-cAMP) is a prodrug for its metabolite, 8-Cl-adenosine which exerts growth inhibition in broad spectrum of cancer cells. The present study was carried out clarify overall cellular affects 8-Cl-cAMP and on SK-N-DZ human neuroblastoma cells by ystematically characterizing gene expression using radioactive cDNA microarray. Microarray prepared with PCR-amplified 2,304 known genes spotted nylon...
Preclinical studies are crucial for developing amyotrophic lateral sclerosis drugs. Current FDA-approved drugs have been created by monitoring limb muscle function and histological analysis of model animals. Drug candidates this disease yet to be tested bulbar-onset type due the limitations traditional preclinical tools: excessive animal use discrete detection progress. Here, our study introduces an all-in-one, wireless, integrated wearable system facilitating continuous drug efficacy...