Abstract Caveolin-1 (CAV1) acts as a growth suppressor in various human malignancies, but its expression is elevated many advanced cancers, suggesting the oncogenic switch of role during tumor progression. To understand molecular basis for growth-promoting function CAV1, we characterized status, differential roles growth, and effect on glucose metabolism colorectal cancers. Abnormal elevation CAV1 was detected substantial fraction primary tumors cell lines tightly correlated with promoter...

Significance Epigenetic inactivation of X-linked inhibitor apoptosis (XIAP)-associated factor 1 (XAF1) is frequently observed in multiple human malignancies. However, the mechanisms underlying its tumor-suppression function remain largely undefined. Here, we identify XAF1 as a positive feedback regulator p53, which directs apoptotic switch p53 signaling. As unique transcription target signaling apoptosis, acts competitor E3 ubiquitin ligase MDM2 binding to and thus disrupts p53–MDM2...

Background: Interest in biological augmentation for improving bone-tendon interface (BTI) healing after arthroscopic rotator cuff repair (ARCR) is growing. Dermal fibroblasts, known collagen synthesis similar to tenocytes, have shown effectiveness BTI chronic tear (RCT) models rabbits. However, no human clinical trials been conducted. Purpose: To evaluate the efficacy of autologous dermal fibroblasts (ADFs) ARCR patients with full-thickness RCTs >2 cm. Study Design: Randomized controlled...

PRKCDBP is a putative tumor suppressor in which alteration has been observed several human cancers. We investigated expression and function of colorectal cells tissues to explore its candidacy as tumorigenesis.Expression methylation status effect on growth were evaluated. Transcriptional regulation by NF-κB signaling was defined luciferase reporter chromatin immunoprecipitation assays.PRKCDBP hardly detectable 29 80 (36%) primary tumors 11 19 (58%) cell lines, correlated with stage grade....

TGF-β1 plays biphasic functions in prostate tumorigenesis, inhibiting cell growth at early stages but promoting malignant progression later stages. However, the molecular basis for oncogenic conversion of function remains largely undefined. Here, we demonstrate that PPARδ is a direct transcription target and critical role redirection signaling. Blockade induction enhances tumor response to TGF-β1-mediated inhibition, while its activation promotes TGF-β1-induced growth, migration invasion....

Significance Epigenetic inactivation of XAF1 tumor suppressor is frequently observed in multiple human cancers. However, the mechanisms underlying its function remain largely undefined. Here, we present evidence that plays a critical role cell-fate decisions under heavy-metal–induced stress conditions through mutual antagonism with MT2A. activated as transcription target MTF-1 and destabilizes MT2A interaction-directed lysosomal degradation, whereas it destabilized by cytostatic conditions....

Background: Tenocytes derived from tendons have been reported to be effective in the treatment of rotator cuff tears through expression extracellular matrix proteins. Human dermal fibroblasts, known express collagen types I and III as tenocytes do, may likely substitutes for enhance healing tears. Purpose: To demonstrate capability human fibroblasts Study Design: Controlled laboratory study. Methods: The cellular properties profiles growth factors were compared between tenocytes. In both...

Abstract To identify and characterize the HDLp (high‐density lipophorin) receptor from Galleria mellonella (LpR Gm ), we used techniques of ligand blotting. This method was, to our knowledge, first lipophorin (LpR) in insects. LpR had an approximate molecular weight 97 kDa under non‐reducing conditions bound specifically. The time‐course binding their protein was rapid. lipophorins receptors saturable with a K d 34.33 ± 4.67 μg/ml. Although Ca 2+ essentially required receptors, interestingly...

ABSTRACT This study was performed to explore novel and valuable uses of insect resources, important subjects the natural compound used in bio‐industries. The whole bodies two crickets, Gryllus bimaculatus Teleogryllus emma , selected from medicinal species, were carefully ground treated with 80% EtOH. extracts solubilized separated by hexane, butanol, D.W according their polarities. Three types extracts, a fraction (G1) boiling extract (G2) an introduced cricket, G. (T1) Korean local T....

This paper introduces technology to fabricate a guided mode resonance filter biochip using injection molding. Of the various nanofabrication processes that exist, molding is most suitable for mass production of polymer nanostructures. Fabrication nanograting pattern filters by requires durable metal stamp, because high temperature and pressure. Careful consideration optimized process parameters also required achieve uniform sub-wavelength gratings with fidelity. In this study, metallic...

There is growing interest in various biological supplements to improve tendon healing patients after arthroscopic rotator cuff repair. The ideal supplement strengthen remains unknown.To assess the safety and efficacy of autologous cultured dermal fibroblast (ADF) injection on tendon-to-bone repair.Case series; Level evidence, 4.Included were 6 who underwent repair between June 2018 March 2020; all had a full-thickness tear (>2 cm) involving supraspinatus infraspinatus tendons. injected with...

The incidence of rotator cuff tears is rapidly increasing, and operative techniques for repair have been developed. However, the rates postoperative retear remain high.The purpose was to determine effects human dermal fibroblasts (HDFs) with hyaluronic acid (HA) on tendon-to-bone healing in a rabbit model chronic tear injury. It hypothesized that HA would enhance HDF proliferation combination HDFs produce synergistic effect repaired tendons rabbits.Controlled laboratory study.For vitro...

Male-specific protein (MSP) is a soluble that accumulates in high amounts the hemolymph and other organs of adult male wax moth. The MSP was purified from moth by gel filtration reversed phase column chromatography, its amino acid sequence determined. Because blocked N-terminus, several internal sequences were obtained in-gel digestion method using trypsin. RT-PCR conducted degenerate primers designed sequences. 5'-RACE PCR used to obtain complete coding region 5'-UTR sequence. full length...

ABSTRACT Complete mRNA sequence of transferrin from Galleria mellonella was obtained, and compared with those other species. Until now, two types insect were reported. Transferrins in cockroach termite have iron binding sites while most groups, studied for the protein, only one. It suggested that presence related evolution, because vertebrate transferrins sites, called N C terminal lobe. shown G. also has one site (N lobe), deduced amino acid similar to Manduca sexta Bombyx mori.

ABSTRACT Tissue‐ferritin was homogeneously purified from the hemolymph‐free whole body of Galleria mellonella larvae. composed four subunits, 26, 30, 32 and 34 kDa. The kDa subunit among them specifically found in tissue ferritin while its N‐terminal sequence 19 amino acid residues identical to that hemolymph‐ferritin subunit. It observed tissue‐ferritin were glycosylated like hemolymph‐ferritin. suggests is an isoform 32kDa according post‐transcriptional modification. By semi quantitative...

ABSTRACT The effect of cricket extract on high‐fat diet fed rat was observed. It shown that prevented the increment body weight by diet. also decreased value AST in liver. most significant lipid metabolism. contents total and cholesterol liver feces were reduced dose‐dependent. These statistically results clear 3% treated group (HFD3) while slight 1% (HFD1). same result fat content.

Abstract Background In organ transplantation, increasing evidence, both in experimental and human studies, indicates that Toll‐like receptor ( TLR ) activation is involved the innate immune recognition of allograft. 5, only protein s, indicated potentially to be regulation target. This study was designed determine whether 5 could a biomarker for vivo allograft visualization, after immunosuppressant rapamycin treatment, using radiolabeled sodium iodide 131 I)‐anti‐ monoclonal antibody (mAb)....