A5052613002- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Toxin Mechanisms and Immunotoxins
- Animal Disease Management and Epidemiology
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Insect Pest Control Strategies
- Toxoplasma gondii Research Studies
- Animal Diversity and Health Studies
- Antibiotic Resistance in Bacteria
- Malaria Research and Control
- Mosquito-borne diseases and control
- Insect Resistance and Genetics
- Pharmaceutical and Antibiotic Environmental Impacts
- Agricultural pest management studies
- Cytomegalovirus and herpesvirus research
- Plant Virus Research Studies
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Immunology Research
- Milk Quality and Mastitis in Dairy Cows
- Antibiotic Use and Resistance
- Phytochemical compounds biological activities
- Brucella: diagnosis, epidemiology, treatment
- Parasitic Infections and Diagnostics
University of Nairobi
2016-2025
Obihiro University of Agriculture and Veterinary Medicine
2007-2016
Chinese Academy of Agricultural Sciences
2009
Qinghai University
2009
Shanghai Veterinary Research Institute
2009
Infections with Babesia bovis, bigemina, Theileria species and Anaplasma marginale are endemic in Kenya yet there is a lack of adequate information on their genotypes. This study established the genetic diversities above tick-borne hemoparasites infecting cattle Kenya.Nested PCR sequencing were used to determine prevalence diversity parasites 192 blood samples collected from Ngong Machakos farms. B. bovis spherical body protein 4, bigemina rhoptry-associated 1a, A. major surface 5, spp. 18S...
Cholera is a public health challenge in Kenya. This study aimed at identifying the spatio-temporal pattern of cholera and its associated risk factors high-risk areas to optimize resource use for targeted control. The was conducted Mukuru, an informal settlement, Dadaab refugee camp counties bordering Lake Victoria. line list data from 32 subcounties (2013-2022) sourced Kenya's Ministry Health. Population water, sanitation hygiene came 2019 census. Space-time scan statistic (SaTScan) were...
Demand for dairy products in sub-Saharan Africa, is expected to triple by 2050, while limited increase supply predicted. This poses significant food security risk low income households. Understanding how the system operates essential identify mitigation measures insecurity impact. study aims determine structure and functionality of Nairobi's using a value chain mapping approach. Primary data were gathered through focus group discussions key informant interviews with stakeholders Nairobi...
The dairy value chain of Nairobi is comprised, in its majority, small-scale independent enterprises that operate within a complex interlinked system. In this complexity, the coordination and power structures system may have major influences on management food safety. Therefore, aim study was to investigate governance structure challenges faced by stakeholders throughout assess their potential implications Qualitative data were collected through focus group discussions key informant...
The association of antimicrobial usage (AMU) with prevalence antimicrobial-resistant (AMR) Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA) in livestock raw milk consumed by pastoralists Kenya remains unclear. We investigated the relationship between AMU and emergence multidrug-resistant (MDR) MRSA livestock. data were obtained using sales records from veterinary pharmacies. was isolated 603 samples various species, sheep, goat, cow, camel reared Isiolo Marsabit...
Abstract Background Anaplasma and Ehrlichia species are tick-borne pathogens of both veterinary public health importance. The current status these pathogens, including emerging such as minasensis platys , infecting cattle in Kenya, remain unclear, mainly because limitation the diagnostic techniques. Therefore, we investigated dairy Nairobi, Kenya using molecular methods. Results A total 306 whole blood samples were collected from apparently healthy cattle. Whole DNA was extracted tested for...
The glyoxalase pathway, which includes two enzymes, 1 and 2 (Glo1 Glo2), is a ubiquitous cellular system responsible for the removal of cytotoxic methylglyoxal produced during glycolysis. Protozoan parasites, including Toxoplasma gondii (T. gondii) tachyzoites, produce because increased glycolytic fluxes. A Glo1 inhibitor such as curcumin could be considered drug candidate anti-protozoan, anti-inflammatory, anti-cancer therapy.The T. gene (TgGlo1) was cloned recombinant protein produced....
Background. The emergence of antimicrobial resistance (AMR) and multidrug (MDR) among Escherichia coli Klebsiella pneumoniae, especially through the production extended spectrum β-lactamases (ESBLs), limits therapeutic options poses a significant public health threat. Objective. aim this study was to assess phenotypic genetic determinants ESBL-producing pneumoniae isolates from patient samples in two Kenyan Hospitals. Methods. We collected 138 E. 127 K. various clinical specimens at...
Background. The widely used methods of diagnosis Toxoplasma gondii are serological. Current reports indicate a high seroprevalence T. in humans Kenya. There is need for more sensitive diagnostic tests, especially when the specific antibody titres below detectable threshold levels. Use polymerase chain reaction (PCR) targeting repetitive 529 base pair loci has been reported to be and specific. Objective. To detect high-risk group public health workers Thika District, Methods. In total, 87...
Abstract Background Identification of risk factors is crucial in Foot-and-mouth disease (FMD) control especially endemic countries. In Rwanda, almost all outbreaks Foot-and-Mouth Disease Virus (FMDV) have started Eastern Rwanda. Identifying the this area will support government efforts. This study was carried out to identify and map different for incursion, spread persistence FMDV Questionnaires were administered during farm visits establish FMD outbreaks. Descriptive statistical measures...
Serological immunoscreening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s other apicomplexan parasites. This corresponded full-length cDNA containing an open reading frame of 1968 bp predicted result in 70-kDa mature protein consisting 656 amino acids. Analysis the expression levels BgHSP-70 indicated elevated transcription cultured parasites incubated at 40 degrees C for 1 h, but not 30 C. Interestingly,...
Although primary infection of mice with Babesia microti has been shown to protect against subsequent lethal by rodhaini, the mechanism behind cross-protection is unknown. To unravel this mechanism, we investigated influence nonlethal B. using different time courses on outcome rodhaini infection. Simultaneous infections these parasites resulted in rapid increases parasitemia, 100% mortality BALB/c mice, as observed control infected alone. In contrast, acute, resolving, and chronic-phase were...
Tick-borne diseases (TBDs) caused by Theileria, Babesia, Anaplasma and Ehrlichia species are common in tropical subtropical regions. In this study, we investigated the presence genetic diversity of Theileria spp., ovis, B. E. ruminantium spp. sheep from Machakos Homa Bay counties Kenya. order to improve diagnosis control ovine TBDs, a total 76 blood samples apparently healthy were screened using polymerase chain reaction (PCR). The assays conducted primers based on 18S rRNA, ovis Major...