Histopathological image analysis of stained tissue slides is routinely used in tumor detection and classification. However, diagnosis requires a highly trained pathologist can thus be time-consuming, labor-intensive, potentially risk bias. Here, we demonstrate potential complementary approach for diagnosis. We show that multiphoton microscopy images from unstained, reproductive tissues robustly classified using deep learning techniques. fine-train four pretrained convolutional neural...

We demonstrate coherent anti-Stokes Raman scattering (CARS) microscopy of lipid-rich structures using a single unamplified femtosecond Ti:sapphire laser and photonic crystal fiber (PCF) with two closely lying zero dispersion wavelengths (ZDW) for the Stokes source. The primary enabling factor fast data acquisition (84 micros per pixel) in proof-of-principle CARS images, is low noise supercontinuum (SC) generated this type PCF, contrast to SC PCF one ZDW. dependence pulse on average input...

We demonstrate a novel miniaturized multimodal coherent anti-Stokes Raman scattering (CARS) microscope based on microelectromechanical systems (MEMS) scanning mirrors and custom miniature optics. A single Ti:sapphire femtosecond pulsed laser is used as the light source to produce CARS, two photon excitation fluorescence (TPEF) second harmonic generation (SHG) images using this microscope. The high resolution distortion-free obtained from various samples such USAF target, fluorescent...

We demonstrate for the first time, a portable multimodal coherent anti-Stokes Raman scattering microscope (exoscope) minimally invasive in-vivo imaging of tissues. This device is based around micro-electromechanical system scanning mirror and miniaturized optics with light delivery accomplished by photonic crystal fibre. A single Ti:sapphire femtosecond pulsed laser used as source to produce CARS, two photon excitation fluorescence second harmonic generation images. The high resolution...

The actual field distribution in superlattices under electric domain formation is investigated by photoluminescence and Raman spectroscopy. From the measured subband spacings, we determine magnitude of that corresponds to resonant alignment subbands adjacent wells. electron occupation higher probed (PL) measurements. Comparing results PL current-voltage characteristics, it shown high-field always nonresonantly coupled with a strength below resonance value. sudden increase current when...

We implement an interferometric method using two angular slits to measure the orbital momentum (OAM) mode spectrum of a partially coherent field. As separation changes, interference pattern for particular OAM is obtained. The visibility this as function equivalent correlation By Fourier transforming obtained from double slit interference, we are able calculate This has potential application characterizing in high-dimensional quantum information protocols.

Purpose: There is limited understanding of the mechanistic effects ionizing radiation (IR) exposure in cataract formation. In this study, we explored IR on reactive oxygen/nitrogen species (ROS and RNS) generation human lens epithelial (HLE) cells as an early key event to long-term damage.Materials methods: HLE cell-line was exposed X-rays at varied doses (0–5 Gy) dose-rates. Cell lysates supernatants were collected 20 h post-exposure analysed for viability, cell cycling metabolites ROS (p,...

Despite the many advances intended to enhance response treatment, survival rate of patients with ovarian cancer has only marginally improved in past few decades. One major cause for this, is lack diagnostics platinum-resistant disease. The goal this study was determine whether Raman micro-spectroscopy conjunction multivariate statistical analysis could discriminate between chemically fixed cisplatin-resistant (A2780cp) and cisplatin-sensitive (A2780s) human carcinoma cells. spectra collected...

We report on Raman scattering by phonon pairs in GaN films grown sapphire substrates plasma-enhanced molecular beam epitaxy. The first order data are consistent with results obtained from bulk crystals of the wurtzite structure. A1 and much weaker E2 symmetry components second have been identified. Two-phonon spectra dominated contributions due to longitudinal optical phonons.

Recent findings in populations exposed to ionizing radiation (IR) indicate dose-related lens opacification occurs at much lower doses (<2 Gy) than indicated protection guidelines. As a result, research efforts are now being directed towards identifying early predictors of degeneration resulting cataractogenesis. In this study, Raman micro-spectroscopy was used investigate the effects varying radiation, ranging from 0.01 Gy 5 Gy, on human epithelial (HLE) cells which were chemically fixed 24...

Raman spectroscopy of blood offers significant potential for label-free diagnostics disease. However, current techniques are limited by the use low laser power to avoid photodegradation blood; this translates a signal noise ratio in spectra. We developed novel flow cell based technique that provides reproducible spectra with high and data acquisition time while ensuring short dwell spot photodamage lysates. show our setup is capable detecting minute changes lysate spectral features from...

Raman microspectroscopy in conjunction with multivariate statistical analysis is a powerful technique for label-free classification of live cells based on their molecular composition, which can be correlated to variations protein, DNA/RNA, and lipid macromolecules.

Silicon photomultipliers (SiPMs) are an emerging solid-state alternative to photomultiplier tubes (PMTs) for low light detection, with similar gain but lower cost and operating voltage. We demonstrate coherent anti-Stokes Raman scattering (CARS) imaging in a side-by-side comparison of uncooled SiPM multialkali PMT as well state-of-the-art cooled GaAsP PMT. determine the optimum reverse-bias voltage acquiring best signal-to-noise ratio (SNR) CARS lipids at ${2850}\;{{\rm cm}^{ - 1}}$2850cm-1....

Up to 70% of ovarian cancer patients are diagnosed with advanced-stage disease and the degree cytoreduction is an important survival prognostic factor. The aim this study was evaluate if Raman spectroscopy could detect from different organs within abdominopelvic region, including ovaries. A probe used interrogate specimens a cohort nine undergoing cytoreductive surgery, four three endometrial cancer. feature-selection algorithm developed determine which spectral bands contributed detection...

Spectral focusing is a well-established technique for increasing spectral resolution in coherent Raman scattering microscopy. However, current methods tuning optical chirp setups using focusing, such as glass rods, gratings, and prisms, are very cumbersome, time-consuming to use, difficult align, all of which limit more widespread use the technique. Here, we report stimulated (SRS) configuration can rapidly tune by utilizing compact adjustable-dispersion TIH53 blocks. By varying height...

Blood is a rich source of potential biomarkers for disease screening, risk assessment, detection, and prognosis. It comprised free-floating erythrocytes, leukocytes, platelets, nutrient-rich fluid plasma. samples are readily accessible in minimally invasive manner compared to solid tissue biopsies; this enables repeated monitoring the health status patient. Components blood that can alter based on include metabolites such as glucose lactic acid, immune markers cytokines antibodies, ions...

Biological dosimetry is a key technique for retrospective radiation that provides individual estimates of absorbed dose ionizing radiation, applicable use in large scale radiological/nuclear event. Current techniques biodosimetry are labor intensive and time consuming not high through-put. In this proof-of-concept study, we developed new approach detecting irradiated blood based on Raman spectroscopy combined with multivariate analysis. Peripheral samples from 8 healthy male female,...

SignificanceAltered lipid metabolism of cancer cells has been implicated in increased radiation resistance. A better understanding this phenomenon may lead to improved treatment planning. Stimulated Raman scattering (SRS) microscopy enables label-free and quantitative imaging cellular lipids but never applied domain.AimWe sought investigate the radiobiological response human breast MCF7 using SRS microscopy, focusing on how affects droplet (LD) distribution morphology.ApproachMCF7 were...

Summary We demonstrate the application of coherent anti‐Stokes Raman scattering microscopy for rapid, label‐free chemical imaging waterborne pathogens. Chemically selective images cryptosporidium were acquired in just a few seconds using microscopy, demonstrating its capability rapid detection at single oocyst level. discuss applicability such technique near‐real time automated water testing system.

Gaussian profile fiber Bragg gratings exhibit narrow-bandwidth transmission peaks with significant group delay at the edge of their photonic bandgap. We demonstrate delays ranging from 0.2 to 5.6 ns a 1.2 cm structure. Simulations suggest such device would be capable enhancing field intensity incoming light by factor 800. Enhancement is confirmed photothermally induced bistability these even sub-milliwatt input powers as much four-fold difference in magnitude responses. The strong...

Abstract Articular cartilage posesses unique material properties due to a complex depth‐dependent composition of sub‐components. Raman spectroscopy has proven valuable in quantifying this through cross‐sections. However, cross‐sectioning requires tissue destruction and is not practical situ. In work, spectroscopy‐based multivariate curve resolution (MCR) was employed porcine samples (n = 12) measure collagen, glycosaminoglycan, water distributions the surface for first time; these were...

Abstract Mitochondria are the metabolic hub of cell, playing a central role in regulating immune responses. Dysfunction mitochondrial reprogramming can occur during bacterial and viral infections compromising hosts’ signaling. Comparative evaluation these alterations response to ligands provide insights into cell’s ability mount pathogen-specific In this study, we used two-photon excitation fluorescence (TPEF) imaging quantify reduced nicotinamide adenine dinucleotide phosphate (NAD(P)H)...