A5040559891- Electromagnetic Fields and Biological Effects
- Skin Protection and Aging
- Effects of Radiation Exposure
- Microbial Inactivation Methods
- Ultrasound and Hyperthermia Applications
- Indoor Air Quality and Microbial Exposure
- Carcinogens and Genotoxicity Assessment
- Radioactive contamination and transfer
- Ocular and Laser Science Research
- Radiation Effects and Dosimetry
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Radiation Therapy and Dosimetry
- Heat shock proteins research
- Radioactivity and Radon Measurements
- Spectroscopy and Chemometric Analyses
- Cannabis and Cannabinoid Research
- Traumatic Ocular and Foreign Body Injuries
- Cell death mechanisms and regulation
- Vitamin C and Antioxidants Research
- Urban Green Space and Health
- Single-cell and spatial transcriptomics
- Bee Products Chemical Analysis
- Vitamin K Research Studies
Health Canada
2006-2024
University of Ottawa
2002-2006
Ottawa Regional Cancer Foundation
2001-2005
Western University
1998
Qutob, S. S., Chauhan, V., Bellier, P. Yauk, C. L., Douglas, G. R., Berndt, Williams, A., Gajda, B., Lemay, E., Thansandote, A. and McNamee, J. Microarray Gene Expression Profiling of a Human Glioblastoma Cell Line Exposed In Vitro to 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 636–644 (2006).The widespread use mobile phones has led public concerns about the health effects associated with exposure radiofrequency (RF) fields. The paramount concern most persons relates...
Purpose: In 2012, the Organization for Economic Cooperation and Development (OECD) formally launched Adverse Outcome Pathway (AOP) Programme. The AOP framework has potential predictive utility in identifying early biological endpoints linked to adverse effects. It uses weight of correlative evidence identify a minimal set measurable key events that link molecular initiating an outcome. AOPs have capability knowledge gaps priority areas future research based on relevance addition, can...
Insulin modulates the differentiation and synthetic activity of osteoblasts, but its mechanisms action are not fully understood.Because ascorbate also influences osteoblast is a cofactor for collagen synthesis, we examined effects insulin on transport metabolism vitamin C in osteoblastic cells.UMR-106 rat osteoblast-like cells accumulated intracellularly when incubated with dehydroascorbic acid (DHAA; oxidized C).Insulin increased intracellular concentration derived from DHAA initial rates...
Chauhan, V., Mariampillai, A., Bellier, P. Qutob, S. S., Gajda, G. B., Lemay, E., Thansandote, A. and McNamee, J. Gene Expression Analysis of a Human Lymphoblastoma Cell Line Exposed In Vitro to an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field. Radiat. Res. 165, 424–429 (2006).This study was designed determine whether radiofrequency (RF) fields the type used for wireless communications could elicit cellular stress response. As general indicators response, we monitored changes in...
Recent findings in populations exposed to ionizing radiation (IR) indicate dose-related lens opacification occurs at much lower doses (<2 Gy) than indicated protection guidelines. As a result, research efforts are now being directed towards identifying early predictors of degeneration resulting cataractogenesis. In this study, Raman micro-spectroscopy was used investigate the effects varying radiation, ranging from 0.01 Gy 5 Gy, on human epithelial (HLE) cells which were chemically fixed 24...
Abstract There is considerable controversy surrounding the biological effects of radiofrequency (RF) fields, as emitted by mobile phones. Previous work from our laboratory has shown no effect related to exposure 1.9 GHz pulse‐modulated RF fields on expression 22 000 genes in a human glioblastoma‐derived cell‐line (U87MG) at 6 h following 4 field period. As follow‐up this study, we have now examined possible late onset U87MG cells after 24 In addition, monocyte‐derived (Mono‐Mac‐6, MM6) was...
Raman spectroscopy of blood offers significant potential for label-free diagnostics disease. However, current techniques are limited by the use low laser power to avoid photodegradation blood; this translates a signal noise ratio in spectra. We developed novel flow cell based technique that provides reproducible spectra with high and data acquisition time while ensuring short dwell spot photodamage lysates. show our setup is capable detecting minute changes lysate spectral features from...
Considerable data has been generated to elucidate the transcriptional response of cells ultraviolet radiation (UVR) exposure providing a mechanistic understanding UVR‐induced cellular responses. However, using these support standards development challenging. In this study, we apply benchmark dose (BMD) modeling derive thresholds gene responsiveness following solar‐simulated UVR. Human epidermal keratinocytes were exposed three doses (10, 20, 150 kJ/m 2 ) solar simulated UVR and assessed for...
Cisplatin is one of the most widely used chemotherapeutics in world today. Unfortunately, chemoresistance often develops hindering effectiveness drug. Mismatch-repair (MMR) and p53 have previously been shown to be important determinants cisplatin resistance can contribute clinically. Here, we cDNA microarray identify several genes as up or downregulated a described, resistant, clone HCT116 cell line (HCT116-K). On follow-up, gene, APM2, was found promote when overexpressed sensitive clones....
Inducible responses are well documented to play a role in the radiation response of cells. However, it is not known whether clinically relevant fractionated X-radiation treatment could elicit an inducible-like radioprotective and there direct correlation between inducible phenomenon intrinsic cell. Therefore, purpose this study was determine closely related human colorectal tumor (HCT116) clones treated with X rays subsequent acute (i.e. single) X-ray challenge, magnitude correlates...
Unprotected exposure to handheld lasers can cause temporary or permanent vision loss depending on the laser classification.To evaluate occurrence of, and details associated with, reported eye injuries resulting from lasers.A 14-item questionnaire developed by Health Canada was distributed Canadian Ophthalmological Society Association of Optometrists their respective members.Questionnaire data were available 909 respondents (263 ophthalmologists; 646 optometrists). Response rates 23.1% 12.7%,...
Qutob, S. S., Multani, A. Pathak, Feng, Y., Kendal, W. and Ng, C. E. Comparison of the X-Radiation, Drug Ultraviolet-Radiation Responses Clones Isolated from a Human Colorectal Tumor Cell Line. Radiat. Res. 161, 326– 334 (2004).We isolated several clones with wide range responses to X radiation an unirradiated human colorectal (HCT 116) tumor cell line. The one these (HCT116-Clone10) nine other either fractionated or acute (i.e. single, nonfractionated doses) irradiation in vitrowas similar...
The objective of this study was to collect prevalence estimates indoor tanning usage and associated injuries in Canada. rapid response component the 2019 Canadian Community Health Survey collected data on use equipment previous 12 months, including reasons for use, frequency/duration precautions taken adverse reactions or injuries. research findings were as follows, an estimated 3.0% (95% CI: 2.5-3.4%) Canadians reported that they had used past year. Among users, 71.1% 63.9-78.3%) female...
Exposure to ultraviolet radiation (UV-R), from both natural and artificial tanning, heightens the risk of skin cancer by inducing molecular changes in cells tissues. Despite established transcriptional alterations at a level due UV-R exposure, uncertainties persist regarding UV characterization subsequent genomic changes. Our study aimed mechanistically explore dose- time-dependent gene expression changes, that may drive short-term (e.g., sunburn) long-term actinic cancer) consequences....
Qutob, S. S., Proulx, D., Mesak, F. M. and Ng, C. E. Effects of N1, N13-Diethylnorspermine (DENSPM) X-Radiation Treatment on Human Colorectal Tumor Clones with Varying Drug Responses. Radiat. Res. 163, 357–363 (2005).This study was designed to examine the effects treatment N13-diethylnorspermine (DENSPM), a spermine analog, X radiation survival polyamine spermidine/spermine N1-acetyltransferase (SSAT) levels in closely related human colorectal tumor (HCT116) clones exhibiting wide range...
To assess the effects of 1800 MHz radiofrequency electromagnetic field (RF-EMF) exposure on expression signal transduction and antioxidant proteins in a human-derived A172 glioblastoma cell line.
We previously isolated several clones that were closely-related genetically from a human colorectal tumor (HCT116) cell line. These displayed significantly different X-radiation response phenotypes. In this paper, we investigated how single dose of modulated the transcriptomic profiles either radiation-resistant (HCT116Clone2_XRR) or radiation-sensitive (HCT116CloneK_XRS) clone when each was compared to reference clone, HCT116Clone10_control. The latter represented control similar as...
This study assessed the use of and exposure to handheld laser devices by Canadians potential associated health risks.The 2019 Canadian Community Health Survey collected data from 12,397 on prevalence or use, eye skin injuries.In 2019, an estimated 12.4% (95% CI: 11.4% 13.4%) reported using a device being exposed its beam in previous year, those between ages 12 17 represented 30.5% 26.6% 34.4%) users. The highest was among with university education (13.8%; 95% 11.8% 15.8%), significant trend...