A5012025769- Adenosine and Purinergic Signaling
- Neuroscience of respiration and sleep
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Neuroinflammation and Neurodegeneration Mechanisms
- Molecular Sensors and Ion Detection
- Vagus Nerve Stimulation Research
- Ion channel regulation and function
- HIV Research and Treatment
- Pancreatic function and diabetes
- Genetics and Neurodevelopmental Disorders
- Heat shock proteins research
- Higher Education Teaching and Evaluation
- Fibroblast Growth Factor Research
- Pharmacological Receptor Mechanisms and Effects
- E-Learning and Knowledge Management
- Parasites and Host Interactions
- Genetic Neurodegenerative Diseases
- Research on Leishmaniasis Studies
- Neurological disorders and treatments
- Electrochemical sensors and biosensors
- Connexins and lens biology
- Sleep and Wakefulness Research
- Autoimmune Neurological Disorders and Treatments
Universidad Complutense de Madrid
2001-2014
Centro de Biología Molecular Severo Ochoa
2009
Universidad Autónoma de Madrid
2009
Consejo Superior de Investigaciones Científicas
2009
The precise mechanism by which mutant huntingtin elicits its toxicity remains unknown. However, synaptic alterations and increased susceptibility to neuronal death are known contributors Huntington's disease (HD) symptomatology. While decreased metabolism has long been associated with HD, recent findings have surprisingly demonstrated reduced apoptosis in Caenorhabditis elegans Drosophila models of HD drugs that diminish ATP production. Interestingly, extracellular recently reported elicit...
The presence of ionotropic P2X 7 receptor has been studied in mice brain from wild type and knockout animals. Western blot immunocytochemical assays show the a protein containing immunogenic epitopes model. Reverse transcriptase polymerase chain reaction experiments demonstrate absence disrupted sequence, but other sequences specific mRNA expression have detected. Functional calcium imaging cultured granule neurons cerebella existence functional ‐like that keeps some properties genuine receptor.
Previously, we reported that purinergic ionotropic P2X7 receptors negatively regulate neurite formation in Neuro-2a (N2a) mouse neuroblastoma cells through a Ca(2+)/calmodulin-dependent kinase II-related mechanism. In the present study used this cell line to investigate parallel though faster receptor-mediated signaling pathway, namely Ca(2+)-regulated exocytosis. Selective activation of evoked exocytosis as assayed by high resolution membrane capacitance measurements. Using dual-wavelength...
Abstract It is currently believed that in each vertebrate species Müller cells the central retina constitutes a fairly homogeneous population from morphologic point of view and particularly chick cell attains full shape differentiation at prenatal stages. However, this study retina, day 1 to 55 life, we show there large variety shapes many them complete postnatally. We used dissociation method preserves whole cells. Unstained living unstained fixed were studied by phase‐contrast microscopy,...
Functional ionotropic nucleotidic receptors responding to diadenosine pentaphospate and nicotinic epibatidine coexpress in 19% of the total rat midbrain cholinergic terminals, as determined by combination immunological microfluorimetric techniques. Activation each independent receptor induces intrasynaptosomal [Ca<sup>2+</sup>]<sub>i</sub> acetylcholine (ACh) release a dose-dependent way. The responses are inhibited antagonists dinucleotide receptors, thus confirming involvement specific...
In cerebral malaria, the most severe complication of both neurotransmission mechanisms and energy metabolism are affected. To understand how metabolic changes modify neurotransmission, we examined P2 receptor expression in a murine model malaria. Quantitative polymerase chain reaction experiments revealed that parasite deposition was greatest cerebellum, compared with other areas brain, suggesting correlation between brain parasitemia loss control movement. Infected mice showed modified...