A5014920470- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Magnesium Oxide Properties and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Monoclonal and Polyclonal Antibodies Research
- Metal-Organic Frameworks: Synthesis and Applications
- Advanced Combustion Engine Technologies
- Cancer-related gene regulation
- Nanoparticle-Based Drug Delivery
- Microfluidic and Bio-sensing Technologies
- Combustion and flame dynamics
- Diet and metabolism studies
- Birth, Development, and Health
- Epigenetics and DNA Methylation
- Fatty Acid Research and Health
- Supramolecular Self-Assembly in Materials
- Sleep and Work-Related Fatigue
- Non-Invasive Vital Sign Monitoring
- Thermochemical Biomass Conversion Processes
- Protease and Inhibitor Mechanisms
- Metallurgical and Alloy Processes
Yingkou Institute of Technology
2024-2025
Institute of Hydrobiology
2024
Chinese Academy of Sciences
2014-2024
University of Chinese Academy of Sciences
2024
University of California, Riverside
2019-2024
Hefei University of Technology
2023-2024
Fudan University
2016-2022
University of California System
2021
University of California, Berkeley
2021
Riverside
2021
Highly selective and efficient capture of glycosylated proteins peptides from complex biological samples is profound significance for the discovery disease biomarkers in systems. Recently, hydrophilic interaction liquid chromatography (HILIC)-based functional materials have been extensively utilized glycopeptide enrichment. However, low amount immobilized groups on affinity material has limited its specificity, detection sensitivity binding capacity glycopeptides. Herein, a novel was...
Abstract Facile preparation of core–shell magnetic metal–organic framework nanospheres by a layer‐by‐layer approach is presented. The have high surface area (285.89 cm 2 g −1 ), large pore volume (0.18 3 two kinds mesopores (2.50 and 4.72 nm), excellent responsivity (55.65 emu structural stability, good dispersibility. combination porosity, hydrophobicity, uniform magnetism was exploited for effective enrichment peptides with simultaneous exclusion molecular weight proteins. were...
The heterogeneity and low abundance of protein glycosylation present challenging barriers to the analysis intact glycopeptides, which is key comprehensively understanding role in an organism. Efficient specific enrichment glycopeptides could help greatly with this problem. Here, we propose a new strategy using boronic acid (BA)-functionalized mesoporous graphene–silica composite (denoted as GO@mSiO2–GLYMO–APB) for isolating from complex biological samples. merits composite, including high...
Remote-controlled nanocarriers for drug delivery are of great promise to provide timely, sensitive and spatiotemporally selective treatments cancer therapy. Due convenient precise manipulation, deep penetration through tissues excellent biocompatibility, near-infrared (NIR) irradiation is a preferred external stimulus triggering the release loaded drugs. In this work, controlled chemo-photothermal synergistic therapy, NIR responsive nanocarrier was fabricated using reduced graphene oxide...
A novel magnetic polymer nanoparticle (Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub>@(HA/CS)<sub>10</sub>–Ti<sup>4+</sup> IMAC) was synthesized for the highly selective and efficient enrichment of phosphopeptides.
The highly selective and efficient capture of heterogeneous types phosphopeptides is critical for comprehensive in-depth phosphoproteome analysis, but it still remains a challenge since the lack affinity material with large binding capacity controllable specificity. Here, new was prepared to improve enrichment endue tunable specificity by introducing guanidyl onto poly(glycidyl methacrylate) (PGMA) modified Fe3O4 microsphere (denoted as Fe3O4@PGMA-Guanidyl). thick polymer shell endows...
Protein glycosylation is a significant participant in mass of biological processes, which pivotal protein post-translational modification. Due to the low contents glycopeptides compared with nonglycopeptides and microheterogeneity sites, highly selective enrichment methods for purification are required comprehensive characterization glycoproteomics. In this work, type magnetic mesoporous phenolic resin (MMP) was prepared using branched polyethylenimine (PEI) as cross-linker from homogeneous...
Y2O3-coated MgO: Ce+3 particles with different precipitants were prepared by the co-precipitation method; phase and morphology of sample characterized XRD, TEM, DTA-TG, apparent activation energy coated was studied thermal analysis kinetics. The results showed that precursors synthesized single-phase multi-phase calcined at 1000 °C for 1.5 h to obtain coating thicknesses about 2.5 nm 5 nm. energies precursor change in three stages calculated using Doyle–Ozawa method Kissinger method. average...
Extracellular vesicles (EVs) play important roles in cell–cell communication and pathological development. Cargo profiling for the EVs present clinical specimens can provide valuable insights into their functions help discover effective EV-based markers diagnostic therapeutic purposes. However, highly abundant complex matrix components pose significant challenges specific identification of low-abundance EV cargos. Herein, we combine asymmetrical flow field-flow fractionation (AF4) with...
In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) plays an essential role in the analysis of polymers. To acquire a more reliable strategy for polymer profiling, we characterized four representative polymers including polyethylene glycol 6000, polyvinylpyrrolidone K12, polyol KPOP-5040, and polyether DL-4000. The preparation methods these samples have been optimized from six aspects, matrix, cationization reagent, solvent, mixing...
Viruses, such as Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), infect hosts and take advantage of host cellular machinery for genome replication new virion production. Identifying elucidating pathways viral infection is critical understanding the development life cycle novel therapeutics. The SARS-CoV-2 N protein RNA (vRNA) packaging in formation. Using our quantitative Förster energy transfer/Mass spectrometry (qFRET/MS) coupled method immunofluorescence imaging, we...
A novel magnetic yolk–shell Fe<sub>3</sub>O<sub>4</sub>@mTiO<sub>2</sub>@mSiO<sub>2</sub> demonstrated the excellent enrichment efficacy and selectivity of endogenous phosphopeptides from human serum.
A novel carbon-functionalized ordered magnetic mesoporous silica composite was designed and synthesized.
Boronic acid-functionalized mesoporous magnetic particles with a hydrophilic surface for multimodal enrichment of glycopeptides glycoproteomics.
α-N-Methylation (Nα-methylation), catalyzed by protein N-terminal methyltransferases (NTMTs), constitutes a crucial post-translational modification involving the transfer of methyl group from