A5024691353- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Microfluidic and Capillary Electrophoresis Applications
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Photosynthetic Processes and Mechanisms
- Herpesvirus Infections and Treatments
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cytomegalovirus and herpesvirus research
- Chemical Synthesis and Analysis
- Sensorless Control of Electric Motors
- Analytical chemistry methods development
- Parkinson's Disease Mechanisms and Treatments
- Infant Nutrition and Health
- Nanocluster Synthesis and Applications
- Biosensors and Analytical Detection
- Enzyme Structure and Function
- Ion-surface interactions and analysis
- Microplastics and Plastic Pollution
Dalian Institute of Chemical Physics
2016-2025
Chinese Academy of Sciences
2016-2025
University of Chinese Academy of Sciences
2013-2025
Xi'an Jiaotong University
2024-2025
Chinese Academy of Agricultural Sciences
2022-2025
Harbin Veterinary Research Institute
2022-2025
Northwest Normal University
2024
Zhejiang University
2022-2024
Sun Yat-sen University
2021-2024
Southeast University
2020-2021
Abstract Background Parkinson’s disease (PD) is a prevalent neurological in the elderly with increasing morbidity and mortality. Despite enormous efforts, rapid accurate diagnosis of PD still compromised. Metabolomics defines final readout genome-environment interactions through analysis entire metabolic profile biological matrices. Recently, unbiased profiling human sample has been initiated to identify novel biomarkers dysfunctional pathways, however, it remains challenge define reliable...
Oral administration of probiotics is a promising method to alleviate inflammatory bowel diseases (IBDs). However, gastrointestinal environmental sensitivity and inferior intestinal colonization hinder the alleviation effect. Here, we developed simple yet effective modified prebiotic-based "shield" (Fe-TA@mGN) composed an Fe3+-tannic acid cross-linking network carboxymethylated β-glucan for arming Escherichia coli Nissle 1917 (EcN@Fe-TA@mGN). The Fe-TA@mGN not only acted as dynamic barrier...
Bacteria swim using a flagellar motor that is powered by stator units. Vibrio spp. are highly motile bacteria responsible for various human diseases, the polar flagella of which exclusively driven sodium-dependent units (PomAB). However, how ion selectivity attained, transport triggers directional rotation unit, and unit incorporated into rotor remained largely unclear. Here, we have determined cryo-electron microscopy structure PomAB. The electrostatic potential map uncovers sodium binding...
Two monolithic polymer columns were directly prepared in the UV-transparent fused-silica capillaries via photoinitiated thiol-yne click polymerization of 1,7-octadiyne (ODY) with a dithiol (1,6-hexanedithiol, 2SH) or tetrathiol (pentaerythriol tetrakis(3-mercaptopropionate), 4SH) within 15 min. The rapid provided time-saving approach to optimize preparation conditions. Then, two porogenic systems diethylene glycol diethyl ether (DEGDE)/tetrahydrofuran (THF) and DEGDE/poly(ethylene glycol)...
Remote-controlled nanocarriers for drug delivery are of great promise to provide timely, sensitive and spatiotemporally selective treatments cancer therapy. Due convenient precise manipulation, deep penetration through tissues excellent biocompatibility, near-infrared (NIR) irradiation is a preferred external stimulus triggering the release loaded drugs. In this work, controlled chemo-photothermal synergistic therapy, NIR responsive nanocarrier was fabricated using reduced graphene oxide...
An efficient strategy of glycopeptide enrichment using metal–organic frameworks by hydrophilic interaction chromatography was demonstrated to analyze <italic>N</italic>-linked glycopeptides in mouse liver.
How mutations impact protein stability and structure dynamics is crucial for understanding the pathological process rational drug design. Herein, we establish a time-resolved native mass spectrometry (TR-nMS) platform via rapid-mixing capillary apparatus monitoring acid-initiated unfolding process. The molecular details in are further profiled by 193 nm ultraviolet photodissociation (UVPD) analysis of structure-informative photofragments. Compared with wild-type dihydrofolate reductase...
Background/Objectives: Despite decades of extensive vaccinations against avian infectious bronchitis virus (IBV) infection, outbreaks caused by constantly emerging variants due to genome recombination between different viral strains, including vaccine occur annually worldwide. The development novel vaccines with favorable safety and effectiveness is required but hindered a limited understanding vaccination IBV. Methods: Here, we performed comprehensive analysis the in vivo dynamics...
Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects from living cells into spectrometer, followed by 193 nm UVPD for structural analysis. Applying this approach to calmodulin (CaM), reveal it adopts more extended compared with purified samples vitro, highlighting...
Stable isotope dimethyl labeling, a widely used method for quantitative proteomics, was extended to five channels the first time. Comprehensive proteome and phosphoproteome quantification validated high accuracy throughput of this five-plex method.
Hybrid monoliths with a macroporous structure were prepared within few minutes via photoinduced thiol-ene polymerization reaction, the surfaces of which showed hydrophobic character. The monolithic column demonstrated good separation performance towards alkylbenzenes, peptides, proteins and BSA tryptic digest in cLC.
A novel hybrid magnetic nanoparticle coated with glycopeptide dendrimers was synthesized and utilized for highly efficient <italic>N</italic>-glycopeptide enrichment.
N-dodecyl β-D-maltoside (DDM), a mild detergent with the ability to maintain enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for N-glycoproteomic analysis of minute protein sample from mouse brain tissue. After combining capillary-based glycoproteomic reactor, 281 N-glycosylation sites were successfully characterized 50 μg tissue, which 110% higher at least than those obtained by conventional strategies.
Abstract Aim Extinctions and coextinctions seriously threaten global plant–pollinator assemblies, thus a better understanding of the geographic variability in their robustness is urgently required. Although patterns species extinction rates are frequently explored, it remains largely unknown how subsequent coextinction risk varies across environments. We hypothesize that variation network to extinctions mediated by modularity – tendency be organized modules strongly interacting because...
Maintaining the protein high-order structures and interactions during transition from aqueous solution to gas phase is essential structural analysis of native mass spectrometry (nMS). Herein, we systematically interrogate effects charge state crown ether (CE) complexation on gas-phase native-like structure by integrating nMS with 193 nm ultraviolet photodissociation (UVPD). The alterations photofragmentation yields residues site distribution fragment ions reveal specific sites sequence...
A reagent pre-organization photochemical trifluoromethylation strategy for probing the functional hotspots inside hydrophobic pockets of native proteins.