A5017341055- Reproductive System and Pregnancy
- COVID-19 Impact on Reproduction
- Pregnancy and preeclampsia studies
- SARS-CoV-2 and COVID-19 Research
- Pregnancy and Medication Impact
- Birth, Development, and Health
- Prenatal Substance Exposure Effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Anesthesia and Neurotoxicity Research
- Pelvic floor disorders treatments
- Preterm Birth and Chorioamnionitis
- Tryptophan and brain disorders
- Neurological Disease Mechanisms and Treatments
- Vaccine Coverage and Hesitancy
University of Kentucky
2022-2025
Introduction The immune compartment within fetal chorionic villi is comprised of Hofbauer cells (HBC) and invading placenta-associated maternal monocytes macrophages (PAMM). Recent studies have characterized the transcriptional profile first trimester (T1) placenta; however, phenotypic functional diversity villous at term (T3) remain poorly understood. Methods To address this knowledge gap, from human tissues obtained full-term, uncomplicated pregnancies were deeply phenotyped using a...
Leukocyte diversity of the first-trimester maternal-fetal interface has been extensively described; however, immunological landscape term decidua remains poorly understood. We therefore profiled human leukocytes from collected via scheduled cesarean delivery. Relative to first trimester, our analyses show a shift NK cells and macrophages T enhanced immune activation. Although circulating decidual are phenotypically distinct, they demonstrate significant clonotype sharing. also report within...
ABSTRACT Alcohol-induced dysregulation of microglial activity is associated with neuroinflammation, cognitive decline, heightened risk for neurodegenerative diseases, alcohol dependence, and escalation drinking. Given the challenge longitudinally sampling primary microglia, we optimized an in vitro method to differentiate peripheral blood mononuclear cells (PBMC) from non-human primates (NHP) into microglia-like (induced-microglia; iMGL). The iMGLs displayed transcriptional profiles distinct...
Objectives/Goals: Opioid use disorder (OUD) in pregnancy and its implications on the maternal-fetal interface has been relatively understudied. Here, we aimed to uncover impact of maternal OUD placental structure, function, inflammatory responses further stratified our findings by hepatitis C (HCV) infection. Methods/Study Population: To address this knowledge gap, collected tissue from healthy pregnancies (control) those with opioid without HCV First, development was assessed gross...
Objective: Preterm birth is a leading global cause of neonatal morbidity and mortality that increasing in incidence. Risk factors include history preterm birth, short cervix or bleeding the current pregnancy, maternal infection, among other factors. Various inflammatory biomarkers differ concentration throughout pregnancy women who ultimately deliver versus at term. Research ongoing regarding relationship between cytokine profiles cervical shortening. This pilot study aimed to discover...
Maternal SARS-CoV-2 infection triggers placental inflammation and alters cord blood immune cell composition. However, most studies focus on outcomes of severe maternal infection. Therefore, we analyzed chorionic villi from newborns unvaccinated mothers who experienced mild/asymptomatic during pregnancy. We investigated rewiring using flow cytometry, single-cell RNA sequencing, functional readouts ex vivo stimulation with TLR agonists pathogens. was associated increased frequency memory T B...
Infection during pregnancy can result in adverse outcomes for both pregnant persons and offspring. Maternal vaccination is an effective mechanism to protect mother neonate into post-partum. However, our understanding of passive transfer antibodies elicited by maternal SARS-CoV-2 mRNA remains incomplete.We aimed evaluate the antibody responses engendered following initial booster doses circulation breastmilk better understand immunization newborn.We collected longitudinal blood samples from...
Few studies have addressed the impact of maternal mild/asymptomatic SARS-CoV-2 infection on developing neonatal immune system. In this study, we analyzed umbilical cord blood and placental chorionic villi from newborns unvaccinated mothers with SARSCoV-2 during pregnancy using flow cytometry, single-cell transcriptomics, functional assays. Despite lack vertical transmission, levels inflammatory mediators were altered in blood. Maternal was also associated increased memory T, B cells,...
OBJECTIVES/GOALS: Maternal opioid use disorder (OUD) is linked to poor fetal outcomes. While it has been established that opioids can cross from maternal circulation, the mechanisms underlying these adverse outcomes remain poorly defined. This study aims uncover OUD-associated immunological changes in and circulation. METHODS/STUDY POPULATION: To effect of OUD on immune system at delivery, we collected blood samples delivery healthy pregnant women (controls) with a diagnosis severe OUD....