A5089331990- Venous Thromboembolism Diagnosis and Management
- Antiplatelet Therapy and Cardiovascular Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Cardiac and Coronary Surgery Techniques
- Cardiac Ischemia and Reperfusion
- Acute Myocardial Infarction Research
- Blood properties and coagulation
- Inflammatory Biomarkers in Disease Prognosis
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Platelet Disorders and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiac Health and Mental Health
- Sepsis Diagnosis and Treatment
- Atrial Fibrillation Management and Outcomes
- Immune Response and Inflammation
- Electroconvulsive Therapy Studies
- Pharmacology and Obesity Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Coronary Interventions and Diagnostics
- RNA Research and Splicing
- Nuclear Structure and Function
- Protease and Inhibitor Mechanisms
- Cardiac, Anesthesia and Surgical Outcomes
Anzac Research Institute
2013-2023
The University of Sydney
2013-2023
Concord Repatriation General Hospital
2013-2022
Institute of Cardiology
2015
Sydney Local Health District
2015
Baird Institute
2013
Royal Prince Alfred Hospital
2013
Concord Hospital
2012
Studies have shown dabigatran to be an effective anticoagulant with acceptable bleeding profile. None the less, these patients do suffer from complications. Unfortunately, there are currently no direct reversal agents or established guidelines on management of in circumstances.We examined effects thrombin generation parameters, after ex-vivo spiking plasma (n = 8) FEIBA(®). These parameters were measured using calibrated automated thrombography (CAT) machine.In our study, we showed ability...
Abstract Colchicine inhibits coronary and cerebrovascular events in patients with artery disease (CAD), although known to have anti-inflammatory properties, its mechanisms of action are incompletely understood. In this study, we investigated the effects colchicine on platelet activation a particular focus via collagen glycoprotein (GP)VI receptor, P2Y 12 procoagulant formation. Therapeutic concentrations vitro (equivalent plasma levels) significantly decreased aggregation whole blood rich...
Colchicine has been demonstrated to reduce cardiovascular death, myocardial infarction (MI), ischemic stroke, and ischemia-driven coronary revascularization in people with artery disease (CAD). These reductions were observed even patients already taking antiplatelet therapy. As well as having anti-inflammatory effects, colchicine demonstrates effects. We propose that colchicine's effects primarily target collagen-induced platelet activation via the collagen receptor, glycoprotein (GP)VI,...
Abstract Platelets play an important role in diseases such as cardiovascular disease and cancer, especially through their release of extracellular vesicles (EVs) thrombosis. The effects the anti-inflammatory drug colchicine on platelets are not well understood. We investigated effect pro-coagulant EVs from under low-level activation. Citrated platelet-rich plasma (PRP) healthy donors was incubated with 2 mM or vehicle at 37°C for 30 minutes gentle rotation. incubation conditions caused mild...
Thrombin (via PAR [protease-activated receptor]-1 and PAR-4) ADP P2Y12 receptors) are potent endogenous platelet activators implicated in the development of cardiovascular disease. We aimed to assess whether pathways alter with aging.We characterized activity community-dwelling volunteers (n=174) following age groups: (1) 20 30 (young); (2) 40 55 (middle-aged); (3) ≥70 years (elderly). Platelet was assessed by aggregometry; flow cytometry (surface markers [P-selectin: alpha granule release,...
Hypercoagulable and/or hypofibrinolytic states are risk factors for venous thromboembolism (VTE) including acute pulmonary embolism. Current screening thrombophilia is targeted towards identifying a specific defect and guidelines recommend population-based rather than individualized strategy anticoagulation treatment. We investigated whether there global hypercoagulable state in long-term survivors of embolism no longer receiving therapeutic utilizing the overall haemostatic potential (OHP)...
Platelets are critical in mediating both rapid responses to injury and the development progression of coronary disease. Several studies have shown that, after prolonged exposure agonists, they produce release inflammatory mediators including interleukin-1β (IL-1β), via classical pathway (NLRP3 inflammasome caspase-1 cleavage active IL-1β) as described for leukocytes. This study aimed determine whether there is IL-1β response soluble platelet agonists such NLRP3- caspase-1-dependent. Using...
Persistent alteration to host polymorphonuclear cell (PMN) physiology has been demonstrated after cardiac surgery performed with cardiopulmonary bypass (CPB). However, date, PMN and function beyond the first 24 h have not investigated without CPB (off-pump coronary artery grafting [OPCAB]). Blood samples of 15 patients were collected preoperatively on days 1, 3, 5 OPCAB. Expression CD11b, CD18, CBRM1/5, CD62L assessed by flow cytometry under resting conditions stimulation formyl...