A5015183624- Complement system in diseases
- Platelet Disorders and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Blood Coagulation and Thrombosis Mechanisms
- Cardiac Valve Diseases and Treatments
- Cardiac Structural Anomalies and Repair
- Immune cells in cancer
- Antiplatelet Therapy and Cardiovascular Diseases
- Infective Endocarditis Diagnosis and Management
- Cardiovascular Function and Risk Factors
- Apelin-related biomedical research
- Cardiac, Anesthesia and Surgical Outcomes
- Transplantation: Methods and Outcomes
- Adipokines, Inflammation, and Metabolic Diseases
- Phagocytosis and Immune Regulation
- Barrier Structure and Function Studies
- Inflammasome and immune disorders
- Chemical Thermodynamics and Molecular Structure
- Lipoproteins and Cardiovascular Health
- Axon Guidance and Neuronal Signaling
- Viral Infections and Immunology Research
- Cerebrovascular and Carotid Artery Diseases
- Antioxidant Activity and Oxidative Stress
- Animal Behavior and Reproduction
- Neuroendocrine regulation and behavior
Universidade Federal de Santa Maria
2024
University of Tübingen
2012-2023
University Medical Centre Mannheim
2023
Heidelberg University
2023
University Hospital Heidelberg
2023
University of Lübeck
2019-2022
University Hospital Schleswig-Holstein
2019-2022
German Centre for Cardiovascular Research
2019
Universitäres Kinderwunschzentrum Lübeck
2019
Universität Ulm
2017
Platelets have distinct roles in the vascular system that they are major mediator of thrombosis, critical for restoration tissue integrity, and players inflammatory conditions. In close spatiotemporal proximity, complement acts as first line defense against invading microorganisms is a key inflammation. Whereas fluid phase cross-talk between coagulation systems well appreciated, understanding pathophysiological implications such interactions still scant.We analyzed coexpression anaphylatoxin...
Background This study analyzed the effects on long-term outcome of residual mitral regurgitation ( MR ) and mean valve pressure gradient MVPG after percutaneous edge-to-edge repair using MitraClip system. Methods Results Two hundred fifty-five patients who underwent were analyzed. Kaplan-Meier Cox regression analyses performed to evaluate impact clinical outcome. A combined end point (all-cause mortality, MV surgery, redo procedure, implantation a left ventricular assist device) was used....
Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an vitro approach to analyze CD11c+ CD11c+-derived ApoE atherosclerosis. The major steps include harvesting mouse bone marrow, plating culture dishes, treating them with differentiation factors, collecting after removal undesirable populations. This protocol can be adapted for different contexts, thus, serving as models diseases cell-specific molecules. For...
Platelets contribute to the regulation of tissue neovascularization, although specific factors underlying this function are unknown. Here, we identified complement anaphylatoxin C5a-mediated activation C5a receptor 1 (C5aR1) on platelets as a negative regulatory mechanism vessel formation. We showed that expressing C5aR1 exert an inhibitory effect endothelial cell functions such migration and 2D 3D tube Growth factor- hypoxia-driven vascularization was markedly increased in C5ar1-/- mice....
Hyperlipidemia is associated with platelet hyperactivity. In the present study, we evaluated binding of oxidized low-density lipoprotein (oxLDL) on surface circulating platelets in patients stable coronary artery disease and acute syndromes its possible association activation. Furthermore, role oxLDL adhesion to collagen endothelial cells vitro as well after carotid ligation mice was investigated.Using flow cytometry, (n=174) showed significantly enhanced compared (n=182; P=0.007)....
Percutaneous edge-to-edge mitral valve repair (PMVR) has become an established treatment option for regurgitation in patients not eligible surgical repair. Currently, most procedures are performed under general anesthesia (GA). An increasing number of centers, however, performing the procedure deep sedation (DS). Here, we compared undergoing PMVR with GA or DS.A total 271 consecutive underwent at our institution between May 2014 and December 2016. Seventy-two were 199 DS. We observed that DS...
Abstract Recent evidence suggests interaction of platelets with dendritic cells (DCs), while the molecular mechanisms mediating this heterotypic cell cross-talk are largely unknown. We evaluated role integrin Mac-1 (αMβ2, CD11b/CD18) on DCs as a counterreceptor for platelet glycoprotein (GP) Ibα. In dynamic coincubation model, we observed human monocyte-derived DCs, but also that activation induced sharp increase in binding. Inhibition CD11b or GPIbα led to significant reduction DC adhesion...
Both oxidized lipids as well the complement system contribute to atherothrombosis. The expression of receptors correlates with platelet activation markers, and bound low-density lipoprotein (oxLDL) modulates function. In present study, we investigated relationship markers activation, anaphylatoxins C5a C3a, lipoprotein.Two hundred seven patients coronary artery disease (CAD) were analyzed in this study. Using enzyme-linked immunosorbent assays, plasma levels oxLDL, measured. Moreover,...
In this study, we evaluated right ventricular (RV) function before and after percutaneous mitral valve repair (PMVR) using conventional echocardiographic parameters novel 3DE data sets acquired prior to directly the procedure. Observational study on 45 patients undergoing PMVR at an university hospital. overall collective, 3D RV-EF showed no significant change (p = 0.16). While there was a increase of fractional area (FAC, from 23 [19-29] % 28 [24-33] %, p 0.001), tricuspid annular plane...
Abstract Aim The aim of this study is to analyse the prognostic value complement anaphylatoxin receptors in patients with non‐ischaemic cardiomyopathy undergoing endomyocardial biopsy. Methods and results In 102 (72.5% male patients, median age 54 years) cardiomyopathy, myocardial expression C3aR was assessed among other parameters. primary endpoint a composite death, heart transplantation, failure‐related re‐hospitalization, deterioration left ventricular ejection fraction within mean...
<b>Introduction</b> The corpus luteum (CL) is dependent on luteal vascular permeability, which controlled by human chorionic gonadotropin (hCG) via endothelial growth factor (VEGF). In this study we investigated the role of a potential VEGF antagonist pathway – Slit2/Robo4 and its influence cell adhesion. <b>Materials Methods</b> Luteinized granulosa cells (LGCs) were stimulated with hCG in absence or presence inhibitor. expression Slit2 measured. Human umbilical vein (HUVECs) VEGF, gene...
Platelets are key mediators of thrombus formation and inflammation during the acute phase ischaemic stroke. Particularly, platelet glycoprotein (GP) receptors GPIbα GPVI have been shown to mediate adhesion activation in brain. blockade could reduce infarct volumes improve functional outcome mouse models stroke, without concomitantly increasing intracerebral haemorrhage. However, role platelets long-term stroke recovery has not elucidated so far. Thus, we here examined impact depletion on...
In ischemic tissue, platelets can modulate angiogenesis. The specific factors influencing this function, however, are poorly understood. Here, we characterized the complement anaphylatoxin C5a-mediated activation of C5a receptor 1 (C5aR1) expressed on as a potent regulator ischemia-driven revascularization. We assessed relevance C5aR1 in patients with coronary artery disease well those peripheral and used genetic mouse models to characterize its significance for ischemia growth factor-driven...
Background: The overall incidence of hospital admissions because myocardial infarction attributable to young patients seams increase over the years. Myocardial in is a rare disease, it necessary identify with high risk profile recognize and treat them time. Case Summary: We present patient an acute heart attack at age several factors for cardiovascular event, including multiple drug abuse hypercholesterolemia. Additionally, had second-degree atrioventricular block severely reduced pumping...
Percutaneous mitral valve repair (PMVR) has evolved to be a standard procedure in suitable patients with regurgitation (MR) not accessible for open surgery. Here, we analyzed the influence of number and positioning clips implanted during on MR reduction analyzing also sub-collectives functional degenerative (DMR).We included 410 severe undergoing PMVR using MitraClip® System. were by TEE at beginning end procedure. To specify clip localization, sub-divided segment 2 into 3 sub-segments...
It is well accepted that drug-eluting stents (DES) are effective in reducing restenosis, although implantation of DES may result increased occurrence stent thrombosis. The study describes the case a patient with very late thrombosis (VLST) despite intravascular ultrasound (IVUS) control after paclitaxel-eluting stent, an adequate response to antiplatelet therapy, and lack distinct risk factors for VLST. Stent remains serious complication even prolonged time, studies urgently needed optimize...