A5074045961- Immune cells in cancer
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Immune responses and vaccinations
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- T-cell and B-cell Immunology
- Systemic Lupus Erythematosus Research
- Virus-based gene therapy research
- Genetic Syndromes and Imprinting
- Sirtuins and Resveratrol in Medicine
- Mycobacterium research and diagnosis
- Genomics and Chromatin Dynamics
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Vitamin C and Antioxidants Research
- Cytokine Signaling Pathways and Interactions
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- vaccines and immunoinformatics approaches
- Autophagy in Disease and Therapy
AstraZeneca (Spain)
2023-2024
Josep Carreras Leukaemia Research Institute
2019-2023
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2020-2022
Institut d'Investigació Biomédica de Bellvitge
2019
The active form of vitamin D, 1,25-dihydroxyvitamin D3, induces a stable tolerogenic phenotype in dendritic cells (DCs). This process involves the D receptor (VDR), which translocates to nucleus, binds its cognate genomic sites, and promotes epigenetic transcriptional remodeling. In this study, we report occurrence D-specific DNA demethylation activation at VDR binding sites associated with acquisition tolerogenesis vitro. Differentiation DCs associates IL-6-JAK-STAT3 pathway. We show that...
Abstract Dendritic cells (DCs), the most potent antigen-presenting cells, are necessary for effective activation of naïve T cells. DCs’ immunological properties modulated in response to various stimuli. Active DNA demethylation is crucial DC differentiation and function. Vitamin C, a known cofactor ten-eleven translocation (TET) enzymes, drives active demethylation. C has recently emerged as promising adjuvant several types cancer; however, its effects on human immune poorly understood. In...
Systemic lupus erythematosus (SLE) is characterised by systemic inflammation involving various immune cell types. Monocytes, pivotal in promoting and regulating SLE, differentiate from classic monocytes into intermediate non-classic monocytes, assuming diverse roles changing their proportions inflammation. In this study, we investigated the epigenetic transcriptomic profiles of these novel monocyte subsets SLE relation to activity progression.
Objective Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease that mainly targets joints. Monocytes and macrophages are critical in RA pathogenesis contribute to inflammatory lesions. These extremely plastic cells respond extracellular signals which cause epigenomic changes define their pathogenic phenotype. Here, we interrogated how DNA methylation alterations monocytes determined by signals. Methods High-throughput analyses of patients with controls vitro cytokine...
Sirtuins 1 and 2 (SIRT1/2) are two NAD-dependent deacetylases with major roles in inflammation. In addition to deacetylating histones other proteins, SIRT1/2-mediated regulation is coupled epigenetic enzymes. Here, we investigate the links between SIRT1/2 activity DNA methylation macrophage differentiation due their relevance myeloid cells. display drastic upregulation during inhibition impacts expression of many inflammation-related genes. this context, abrogates gains, but does not affect...
Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic (DCs), central actors for coordinating responses, acquire tolerogenic properties response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment various inflammatory diseases. To date, underlying cell type-specific regulatory mechanisms orchestrating GC-mediated acquisition of immunosuppressive remain poorly understood. In this study, we...
COVID-19 manifests with a wide spectrum of clinical phenotypes, ranging from asymptomatic and mild to severe critical. Severe critical patients are characterized by marked changes in the myeloid compartment, especially monocytes. However, little is known about epigenetic alterations that occur these cells during hyperinflammatory responses patients. In this study, we obtained DNA methylome transcriptome peripheral blood monocytes samples extracted CD14 + CD15- 48 11 healthy controls were...
Illumina DNA methylation bead arrays provide a cost-effective platform for the simultaneous analysis of high number human samples. However, can be time-demanding and requires some computational expertise. shinyÉPICo is an interactive, web-based, graphical tool that allows user to analyze (450k EPIC), from user's own computer or server. The covers entire analysis, raw data final list differentially methylated positions regions between sample groups. It test several normalization methods,...
Microbial challenges, such as widespread bacterial infection in sepsis, induce endotoxin tolerance, a state of hyporesponsiveness to subsequent infections. The participation DNA methylation this process is poorly known. In study, we perform integrated analysis and transcriptional changes following vitro exposure gram-negative lipopolysaccharide, together with ex vivo monocytes from septic patients. We identify TET2-mediated demethylation activation inflammation-related genes that specific...
<title>Abstract</title> <bold>Background:</bold> Monocyte subsets, classified into classical, intermediate, and non-classical, have distinct specific functions. Despite increasing reports describing changes in monocyte subsets a wide range of pathological inflammatory conditions, such as autoimmune infectious diseases, the regulatory mechanisms underlying their differentiation are poorly understood. This information could be essential to understand contribution pathology....
Abstract Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by systemic inflammation involving various immune cell types. Monocytes, pivotal in promoting and regulating SLE, differentiate from classical monocytes into intermediate non-classical monocytes, assuming diverse roles. In this study, we investigated the epigenetic transcriptomic profiles of these three monocyte subsets SLE cohort. addition to common DNA methylation alterations, identified subset-specific...
ABSTRACT Dendritic cells (DCs) are central in the immune system, bridging adaptive and innate responses. Research on vitro differentiation of DCs from monocytes provides both in-depth understanding analogous vivo process potential sources for cancer cell therapy. Active DNA demethylation is crucial DC differentiation. Vitamin C a known cofactor ten-eleven translocation (TET) enzymes, which drive active demethylation. Currently, effects vitamin treatment human poorly understood. In this...
Abstract COVID-19 manifests with a wide spectrum of clinical phenotypes, ranging from asymptomatic and mild to severe critical. Severe critical patients are characterized by marked changes in the myeloid compartment, especially monocytes. However, little is known about epigenetic alterations that occur these cells during hyperinflammatory responses patients. In this study, we obtained DNA methylome transcriptome peripheral blood monocytes samples extracted CD14+CD15-monocytes 48 11 healthy...
The active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), induces stable tolerogenic phenotype in dendritic cells (DCs). This process involves the D receptor (VDR), which translocates to nucleus, binds its cognate genomic sites and promotes epigenetic transcriptional remodeling. In this study, we report occurrence D-specific DNA demethylation activation at VDR binding associated with acquisition tolerogenesis. Tolerogenic properties DCs are acquired together IL6-JAK-STAT3...
ABSTRACT Microbial challenges, such as widespread bacterial infection, induce endotoxin tolerance. This state of hyporesponsiveness to subsequent infections is mainly displayed by monocytes and macrophages. Endotoxin tolerance generally acquired following a septic episode. In this study, we investigated DNA methylation changes during the acquisition in vitro We identified set TET2-mediated demethylation events that are specific toll-like receptor (TLR) 2 TLR4 stimulation. Lipopolysaccharide...
Abstract Glucocorticoids (GCs) exert potent anti-inflammatory effects in immune cells through the glucocorticoid receptor (GR). Dendritic (DCs), central actors for coordinating responses, acquire tolerogenic properties response to GCs. Tolerogenic DCs (tolDCs) have emerged as a potential treatment various inflammatory diseases. To date, underlying cell type-specific regulatory mechanisms orchestrating GC-mediated acquisition of immunosuppressive remain poorly understood. In this study, we...