A5073381405- Immune Response and Inflammation
- Asthma and respiratory diseases
- Inflammation biomarkers and pathways
- Immune cells in cancer
- Sepsis Diagnosis and Treatment
- Immune responses and vaccinations
- Respiratory viral infections research
- IL-33, ST2, and ILC Pathways
- Cancer-related molecular mechanisms research
- Neonatal Respiratory Health Research
- MicroRNA in disease regulation
- Eosinophilic Disorders and Syndromes
- Cytokine Signaling Pathways and Interactions
- Eosinophilic Esophagitis
- Pediatric health and respiratory diseases
- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Inflammasome and immune disorders
- Congenital Diaphragmatic Hernia Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Extracellular vesicles in disease
Hospital Universitario Fundación Jiménez Díaz
2022-2024
Universidad Autónoma de Madrid
2022-2024
Josep Carreras Leukaemia Research Institute
2019-2021
Institut d'Investigació Biomédica de Bellvitge
2019
Abstract Background Sepsis, a life-threatening organ dysfunction caused by dysregulated systemic immune response to infection, associates with reduced responsiveness subsequent infections. How such tolerance is acquired not well understood but known involve epigenetic and transcriptional dysregulation. Methods Bead arrays were used compare global DNA methylation changes in patients sepsis, non-infectious inflammatory syndrome, healthy controls. Bioinformatic analyses performed dissect...
MicroRNAs are non-coding molecules that act both as regulators of the epigenetic landscape and biomarkers for diseases, including asthma. In era personalized medicine, there is a need novel disease-associated can help in classifying diseases into phenotypes treatment selection. Currently, severe eosinophilic asthma one most widely studied clinical practice, many patients require higher doses corticosteroids, which some cases fail to achieve desired outcome. Such may only benefit from...
Nowadays, microRNAs (miRNAs) are increasingly used as biomarkers due to their potential contribution the diagnosis and targeted treatment of a range diseases. The aim study was analyze miRNA expression profiles in serum lung tissue from patients with severe asthma treated oral corticosteroids (OCS) those without OCS treatment. For this purpose, miRNAs non-OCS asthmatic individuals were evaluated by miRNAs-Seq, subsequently validation performed using RT-qPCR. Additionally, pathway enrichment...
Eosinophils are leukocytes involved in homeostasis and diseases like asthma. The existence of eosinophil subpopulations remain controversial, with eosinophils being classified as type 1, 2, inflammatory, resident, homeostatic, or developmental depending on the publication,1 no clear indication intrinsic environmental dependence. Unsupervised methods could provide data true subclassifications, which be importance for efficacy asthma therapy.2 Therefore, aim this study is to analyse blood...
Microbial challenges, such as widespread bacterial infection in sepsis, induce endotoxin tolerance, a state of hyporesponsiveness to subsequent infections. The participation DNA methylation this process is poorly known. In study, we perform integrated analysis and transcriptional changes following vitro exposure gram-negative lipopolysaccharide, together with ex vivo monocytes from septic patients. We identify TET2-mediated demethylation activation inflammation-related genes that specific...
Respiratory diseases such as bronchiolitis, and those with wheezing episodes, are highly important during infancy due to their potential chronicity. Immune response dysregulation is critical in perpetuating lung damage. Epigenetic modifications including microRNA (miRNA) post-transcriptional regulation among the factors involved alleviating inflammation. We evaluated expression of miR-146a-5p, a previously described negative regulator immunity, infants respiratory diseases, order study...
Background MicroRNAs are noncoding molecules that act both as regulators of the epigenetic landscape and biomarkers for diseases, including asthma. In era personalized medicine there is a need novel disease-associated can help in classifying diseases into phenotypes treatment selection. Currently, severe eosinophilic asthma one most widely studied clinical practice, many patients require higher doses corticosteroids, which some cases fail to achieve desired outcome. Such may only be benefit...
Clarifying inflammatory processes and categorising asthma into phenotypes endotypes improves management. Obesity worsens severe reduces quality of life, although its specific molecular impact remains unclear. We previously demonstrated that hsa-miR-26a-1-3p hsa-miR-376a-3p, biomarkers related to an profile, discriminate eosinophilic from non-eosinophilic asthmatics. aimed study hsa-miR-26a-1-3p, their target genes in asthmatic subjects with or without obesity find comprehend obese...
ABSTRACT Microbial challenges, such as widespread bacterial infection, induce endotoxin tolerance. This state of hyporesponsiveness to subsequent infections is mainly displayed by monocytes and macrophages. Endotoxin tolerance generally acquired following a septic episode. In this study, we investigated DNA methylation changes during the acquisition in vitro We identified set TET2-mediated demethylation events that are specific toll-like receptor (TLR) 2 TLR4 stimulation. Lipopolysaccharide...
Background: Respiratory viral infections (RVIs) are frequent in preterm infants and may have long-term impact on respiratory morbidity, especially those with bronchopulmonary dysplasia (BDP). The immune response key defence elements, so the purpose of this study is to evaluate regulation epithelial barrier integrity suffering RVIs during Neonatal Intensive Care Unit (NICU) admission. Materials methods: Nasopharyngeal aspirate (NPA) was obtained, separating cells from supernatants. Viral...
Respiratory viral infections (RVIs) are frequent in preterm infants possibly inducing long-term impact on respiratory morbidity. Immune response and barriers key defense elements against insults premature admitted to Neonatal Intensive Care Units (NICUs). Our main goals were describe the local immune secretions of with RVIs during NICU admission evaluate expression synthesis lung barrier regulators, both samples vitro models. Samples from that went develop had lower filaggrin gene protein...