AbstractBetween June 1989 and December 2005, an observational study of adults co-infected with HIV Trypanosoma cruzi was conducted, to investigate the spectrum manifestations chronic Chagas disease (American trypanosomiasis) in HIV-positive. The 31 men 22 women investigated were aged 23–59 years. Each subject by ambulatory (Holter) non-ambulatory electrocardiography, chest X-ray, oesophagography echocardiography (to determine clinical form trypanosomiasis), xenodiagnosis, blood culture...

Journal Article Reactivation of Chagas disease manifested by skin lesions in a patient with AIDS Get access Ana Marli C. Sartori, Sartori 1Clinic Infectious and Parasitic Diseases, University São Paulo School Medicine, Paulo, Brazil Address for correspondence: Christovam R. José Maria Lisboa, 695/91, SP, 01423-001, Brazil; fax +55 11 853 1755, e-mail amsartori@sti.com.br Search other works this author on: Oxford Academic PubMed Google Scholar Mirian N. Sotto, Sotto 2Department Dermatology,...

The congenital transmission of Chagas’ disease was evaluated in 57 pregnant women with and their 58 offspring. patients were selected from three Health Institutions São Paulo City. maternal clinical forms were: indeterminate (47.4%), cardiac (43.8%) digestive (8.8%); 55 born endemic areas two at fetal level confirmed (5.17%) the cases studied one probably case disease. Two infected infants to chagasic HIV infection diagnosed by parasitolological assays (microhematocrit, quantitative buffy...

Rheumatoid arthritis (RA) is a chronic condition that frequent in patients living tropical areas exposed to leishmaniasis. RA therapy involves immunosuppressant drugs such as methotrexate (MTX), monoclonal antibodies (mAbs) and prednisone. We report an unusual presentation of cutaneous (CL) or mucocutaneous leishmaniasis (ML) from endemic area A 51-year-old woman noted ulcer on her left ankle during MTX prednisone therapy. Initially diagnosed venous stasis ulcer, the aspirate injury revealed...

Blastocistose é a infecção causada pelo Blastocystis hominis. Está relacionada com várias controvérsias e indefinições, sem dúvida muito importantes sobretudo pelas implicações médico-assistenciais que suscitam. A propósito, pendência expressiva diz respeito à patogenicidade do referido protozoário, precisa ser categoricamente definida. Ao lado dessa particularidade outros assuntos exigem elucidações através de pesquisas bem conduzidas, para blastocistose fique devidamente situada no...

Three drugs - pentavalent antimonials, amphotericin B and pentamidine are currently used for leishmaniasis treatment. They administered long periods, only parenterally, have high cardiac, renal hepatic toxicities. Therefore, the investigation of new compounds is required. Nitro-heterocyclic derivatives been as possible drug candidates to treat diseases caused by trypanosomatids.Leishmania (L.) amazonensis promastigotes (MHO/BR/73/M2269), maintained in Laboratório de Soroepidemiologia...

SUMMARY It is important to develop new methods for diagnosing relapses in the co-infection of visceral leishmaniasis (VL) and HIV enable earlier detection using less invasive methods. We report a case co-infected patient who had after VL treatment, where qualitative kDNA PCR showed good performance. The seems be useful tool may marker predicting treatment patients with clinical symptoms disease.

The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace invasive painful bone marrow collection (BM) visceral leishmaniasis (VL). PB from suspected VL patients evaluated by techniques using as gold standard (GS) a combination clinical, epidemiological, immunochromatographic test (PB-rK39) results examination BM. Based on GS, 38 samples 32 were grouped: Group 1, 20 cases, 2, 18 non-VL cases. In order PB,...

Amostras de fezes 140 cães adultos da Grande São Paulo (Estado Paulo, Brasil) foram examinadas com a finalidade procurar oocistos Cyclospora sp.. Nenhuma infecção por esse coccídeo ficou detectada.

Para diagnosticar a infecção intestinal pelo Cryptosporidium sp, hoje bastante em foco pela não incomum participação no contexto da síndrome imunodeficiência adquirida (AIDS), várias técnicas têm sido indicadas. Com o intuito de esclarecer vantagens e desvantagens, envolvendo quantificação, morfologia, durabilidade do esfregaço tempo preparação, comparamos os resultados obtidos mediante emprego dois desses processos, ou seja, Kinyoun modificado Heine, aplicados amostras fecais pacientes com...

Em Catolé do Rocha (Estado da Paraíba, Brasil) várias pessoas foram concomitantemente acometidas de doença Chagas, em fase aguda. A propósito desse evento, houve cogitação que o Trypanosoma cruzi foi veiculado pela via digestiva, através ingestão caldo cana acidentalmente contaminado. Com presente estudo, verificamos parasita permaneceu viável, durante quatro horas, no líquido questão mantido temperatura ambiente. Ao mesmo tempo, comprovamos após uma hora ele pôde promover infecção...

Trypanosoma cruzi parasitemia observed in immunocompromised patients (transplant or positive HIV) occurred more frequently by the artificial xenodiagnosis method (10/38) compared with hemoculture (2/38), given same quantity of blood. Other ways diagnosis, like mice inoculation (5/38), QBC and buffy coat were evaluated also. This result showed importance xenodiagnosis. The other techniques increased only one patient positive.

A rapid DNA extraction was used for T. cruzi detection in triatomines dry fecal spots collected on filter paper and analyzed by PCR. Fifty infestans were fed experimentally infected Balb/C mice with high parasitemia divided into five groups of ten triatomines, 100 lower 20 triatomines. One spot per group days 1, 2, 3, 4 5 post feeding. Amplification targeted TCZ sequence resulted positive from day after bugs feeding the two models (high parasitemial. The isolation PCR proposed are suitable...

Foi avaliada a eficácia da administração prolongada de azitromicina e pirimetamina em camundongos infectados com cepa cistogênica Toxoplasma gondii. Os animais foram inoculados intraperitonealmente um cisto T. gondii e, após 20 dias, divididos quatro grupos: GI não tratados, GII tratados concomitantemente (12,5mg/kg/dia) (100mg/kg/dia), GIII mesma dose GIV forma azitromicina. O tratamento, via oral, estendeu-se por 120 dias; este período os sacrificados foi feita contagem dos cistos no...

Foi comprovada a eficácia da azitromicina e pirimetamina no tratamento infecção experimental de camundongos pelo Toxoplasma gondii. Houve administração cotidiana, pela via oral 200mg/kg 12,5mg/kg, respectivamente durante dez dias. O uso dos medicamentos ocorreu mediante associação ou não o emprego concomitante proporcionou melhor resultado convirá efetuar investigação semelhante com cepa cistogênica do parasita.

A 31-year-old male patient developed an ulcer on the glans penis that evolved for three months without healing. We diagnosed it as leishmaniasis using polymerase chain reaction. No immunosuppression or associated diseases were observed. The was treated with meglumine antimoniate cured lesion in a month post-treatment. Here, we report this case of cutaneous at unusual location immunocompetent individual. likely due to bite vector, highlighting need considering among differential diagnosis...

Em modelo experimental, baseado na infecção de ratos pelo Strongyloides venezuelensis, foi avaliada a atividade terapêutica duas preparações ivermectina, para usos veterinário e humano. Houve interesse em verificar efetividade relação vermes adultos formas larvárias. A administração dos fármacos ocorreu sempre por via oral posologia correspondeu à dose única 0,2mg/kg. Considerados os as larvárias, o produto emprego propiciou eliminações expressas pelas porcentagens 98,0% 84,2%; quanto outra...

Em virtude da conveniência de tentar aprimorar o tratamento toxoplasmose, semelhanças taxonômicas e informes contidos na literatura científica, foi considerado oportuno verificar se a artemisinina, medicamento eficiente no sentido poder debelar malária, é ativa infecção experimental camundongos pelo Toxoplasma gondii. Houve, então, administração diferentes quantidades desse antiparasitário, inclusive após prévio contato in vitro com toxoplasmas estimulação produção macrófagos, para...

Diagnosis of the human cyclosporiasis is reported in São Paulo, SP, Brasil. Cyclospora cayetanensis has been identified feces a patient by modified Kinyoun staining method, with later sporulation solution 2.5% potassium dichromate. The probability that this parasite eventual cause gastrointestinal disturbances country was stimulated finding, which arrived at simple technique. It had kept mind disease expressing itself mainly among immunocompromised patients, whose number increasing;...