A5079268639- Genomics and Phylogenetic Studies
- Microbial Natural Products and Biosynthesis
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Actinomycetales infections and treatment
- Mycorrhizal Fungi and Plant Interactions
- Plant-Microbe Interactions and Immunity
- Microbial Community Ecology and Physiology
- Fungal Biology and Applications
- Synthetic Organic Chemistry Methods
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- RNA and protein synthesis mechanisms
- Phytochemical compounds biological activities
- Insect symbiosis and bacterial influences
- Marine Sponges and Natural Products
- Legume Nitrogen Fixing Symbiosis
- Protist diversity and phylogeny
- Plant Pathogens and Resistance
- Diatoms and Algae Research
- Insect and Arachnid Ecology and Behavior
- Entomopathogenic Microorganisms in Pest Control
- Plant Pathogenic Bacteria Studies
- Fungal Plant Pathogen Control
- Traditional and Medicinal Uses of Annonaceae
Northeast Agricultural University
2016-2025
Baoding University
2023-2024
Chinese Academy of Agricultural Sciences
2016-2023
Institute of Plant Protection
2016-2023
Center for Excellence in Molecular Plant Sciences
2018-2023
Chinese Academy of Sciences
2018-2023
Kunming Institute of Botany
2018-2023
China Tobacco
2012-2022
Kunming University
2018
Institute of Forestry
2015
A Gram-positive, aerobic actinomycete, designated strain NEAU 119 T , was isolated from the rhizosphere of a fig tree and characterized using polyphasic approach. The isolate formed branching, non-fragmenting vegetative hyphae produced black pigment on yeast extract/malt extract (ISP medium 2). G+C content DNA 76.6 mol%. organism had chemotaxonomic characteristics typical genus Actinoalloteichus closely related to type strains cyanogriseus spitiensis hymeniacidonis currently only three...
A new prenylated indole derivative 3-acetonylidene-7-prenylindolin-2-one (1) was isolated from the endophytic actinobacterium Streptomyces sp. neau-D50, together with four known hybrid isoprenoids, 7-isoprenylindole-3-carboxylic acid (2), 3-cyanomethyl-6-prenylindole (3), 6-isoprenylindole-3-carboxylic (4) and 7,4′-dihydroxy-5-methoxy-8-(γ,γ-dimethylallyl)-flavanone (5). The structures of these compounds were elucidated on basis extensive spectroscopic analysis comparison data literature....
The present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on actinobacterial community associated with soybean roots. A total 70 endophytic actinobacteria were isolated from surface-sterilized roots either healthy or diseased soybeans, and they distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium Cellulosimicrobium, endemic samples, more diverse in samples compared...
In this study, the supernatant extract from fermentation broth of Streptomyces sp. NEAU-H3 showed strong antifungal activity against Fusarium graminearum strain PH-1 in vitro and vivo. Three known pyranonaphthoquinones were isolated by means an activity-guided method, frenolicin B was characterized as main active ingredient. Frenolicin displayed F. with EC50 value 0.51 mg/L, which is lower than that carbendazim (0.78 mg/L) but higher phenamacril (0.18 mg/L). could also strongly inhibit...
Food is a fundamental human right, and global food security threatened by crop production. Plant growth regulators (PGRs) play an essential role in improving yield quality, this study reports on novel PGR, termed guvermectin (GV), isolated from plant growth-promoting rhizobacteria, which can promote root coleoptile growth, tillering, early maturing rice. GV nucleoside analogue like cytokinin (CK), but it was found that significantly promoted hypocotyl different the function of CK...
Abstract Angiotensin-converting enzyme inhibitors are widely used for treatment of hypertension and related diseases. Here, six karnamicins E 1 -E 6 ( – ), which bear fully substituted hydroxypyridine thiazole moieties characterized from the rare actinobacterium Lechevalieria rhizosphaerae NEAU-A2. Through a combination isotopic labeling, genome mining, enzymatic characterization studies, programmed assembly moiety in karnamicin is proposed to be due sequential operation hybrid polyketide...
A novel actinomycete, designated strain NEAU-Z6(T), was isolated from eggplant (Solanum melongena L.) root. Phylogenetic analysis based on the 16S rRNA gene sequence showed that NEAU-Z6(T) belonged to genus Nonomuraea, with highest similarity Nonomuraea monospora PT 708(T) (98.83 %), rosea GW 12687(T) (98.55 %) and rhizophila YIM 67092(T) (98.02 %). Sequence similarities between other species of ranged 97.94 % (Nonomuraea candida HMC10(T)) 96.30 wenchangensis 210417(T)). Key morphological,...
Actinobacteria associated with insects represent one potentially rich source of novel natural products antifungal activity. Here, we investigated the phylogenetic diversity and community composition actinobacteria ants using a combination culture-dependent -independent methods. Further, assessed antagonistic activity against phytopathogenic fungi identified secondary metabolites from isolates bioactivity. A total 416 actinobacterial were obtained three ant species (Camponotus japonicus,...
Rice direct seeding technology has been considered as a promising alternative to traditional transplanting because of its advantages in saving labor and water. However, the poor emergence seedling growth caused by chill stress are main bottlenecks wide-scale adoption direct-seeded rice Heilongjiang Province, China. Here, we found that natural plant regulator guvermectin (GV) effectively improved seed germination under chilling stress. Results from 2 year field trials showed seed-soaking with...
Plant growth regulators (PGRs) play an important role in alleviating the detrimental effects of biotic and abiotic stress improving crop yield quality. As a novel PGR from Streptomyces registered 2021, guvermectin (GV) has potential to improve plant defense, making its application agriculture subject interest. Here, we describe discovery process, functional activities, agricultural applications, toxicity, environmental safety, biosynthetic mechanism GV. This Perspective provides guide for...
In this study, an endophytic Streptomyces sp. neau-D50 with strong antifungal activity against Phytophthora sojae was isolated from healthy soybean root, using in vitro screening technique. A bioactivity-guided approach then employed to isolate and determine the chemical identity of bioactive constituents strain neau-D50. The structure metabolite elucidated as borrelidin on basis spectral analysis. To our knowledge, is first report that has dominant race 1 P. EC50 EC95 0.0056 0.026 mg/L,...
The rhizosphere, an important battleground between beneficial microbes and pathogens, is usually considered to be a good source for isolation of antagonistic microorganisms. In this study, novel actinobacteria with broad-spectrum antifungal activity, designated strain NEAU-H2T, was isolated from the rhizosphere soil wheat (Triticum aestivum L.). 16S rRNA gene sequence similarity studies showed that NEAU-H2T belonged genus Streptomyces, high similarities Streptomyces rhizosphaerihabitans NBRC...
A novel ikarugamycin-producing actinomycete, designated strain NEAU-Da3(T), was isolated from soybean root [Glycine max (L.) Merr.] and characterized using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that NEAU-Da3(T) belonged to the genus Streptomyces, most closely related Streptomyces carpaticus NRRL B-16359(T) (99.5 %), cheonanensis VC-A46(T) (99.3 %) xiamenensis MCCC 1A01550(T) (97.2 %); similarities other type strains of species were lower than 97.1 %. The...
Borrelidin has high and specific antifungal activity against Phytophthora sojae . To explore the mechanism of borrelidin P. , relationship between concentration threonine was evaluated. The results demonstrated that growth-inhibitory effect on growth antagonized by in a dose-dependent manner, suggesting threonyl-tRNA synthetase (ThrRS) may be potential target borrelidin. Subsequently, inhibition enzymatic ThrRS vitro confirmed. Furthermore, detailed interaction investigated using...
A novel endophytic actinomycete, designated strain NEAU-J5(T) was isolated from roots of snap bean (Phaseolus vulgaris L.). Comparative analysis the 16S rRNA gene sequence indicated that is phylogenetically related to members family Micromonosporaceae. The whole-cell sugars were galactose, mannose and glucose. predominant menaquinones MK-9(H4) MK-9(H6). major fatty acids C16:0, C18:0, C17:1ω7c, iso-C15:0 C17:0. phospholipids phosphatidylmethylethanolamine, phosphatidylethanolamine,...
A novel endophytic actinomycete, designated strain NEAU-LZS-5(T), was isolated from the leaf of candelabra aloe (Aloe arborescens Mill) and characterized using a polyphasic approach. Analysis 16S rRNA gene sequence showed that NEAU-LZS-5(T) belongs to genus Streptomyces exhibited 99.51 97.37 % similarity sedi YIM 65188(T) specialis GW41-1564(T), respectively, whereas low values (<97 %) distinguished all other species with validly published names. Two tree-making algorithms also supported...