A5049451705- Microbial Natural Products and Biosynthesis
- Phytochemistry and Biological Activities
- Plant-derived Lignans Synthesis and Bioactivity
- Bioactive Natural Diterpenoids Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Traditional and Medicinal Uses of Annonaceae
- Plant-based Medicinal Research
- Marine Sponges and Natural Products
- Synthetic Organic Chemistry Methods
- Plant biochemistry and biosynthesis
- Cholinesterase and Neurodegenerative Diseases
- Natural product bioactivities and synthesis
- Biological Activity of Diterpenoids and Biflavonoids
- Phytochemical compounds biological activities
- Carbohydrate Chemistry and Synthesis
- Fungal Biology and Applications
- Thermochemical Biomass Conversion Processes
- Genomics and Phylogenetic Studies
- Alkaloids: synthesis and pharmacology
- Chemical synthesis and alkaloids
- Cancer therapeutics and mechanisms
- Plant-Microbe Interactions and Immunity
- Bioactive Compounds and Antitumor Agents
- Medicinal Plant Extracts Effects
Scripps Research Institute
2012-2025
Yantai University
2025
Kunming Institute of Botany
2015-2024
Chinese Academy of Sciences
2015-2024
Chengdu University of Traditional Chinese Medicine
2010-2024
Center for Excellence in Molecular Plant Sciences
2018-2023
Hunan Agricultural University
2016-2023
Hunan University of Traditional Chinese Medicine
2022
Shaanxi Normal University
2021
Kunming University
2018-2021
Understanding translational control in gene expression relies on precise and comprehensive determination of translation initiation sites (TIS) across the entire transcriptome. The recently developed ribosome-profiling technique enables global analysis, providing a wealth information about both position density ribosomes mRNAs. Here we present an approach, sequencing, applying parallel ribosome E-site inhibitors lactimidomycin cycloheximide to achieve simultaneous detection elongation events...
Dissecting HCMV Gene Expression Most of us are infected with human cytomegalovirus (HCMV), but severe disease is almost always limited to immunocompromised individuals or newborn infants. The virus has a relatively large (∼240 kb) DNA genome and shows complex pattern gene transcription, hinting at regulatory coding capacity. Stern-Ginossar et al. (p. 1088 ) mapped ribosome positions on transcripts during the course viral infection fibroblast cells. data suggest presence novel open reading...
A novel deep red phosphor Ca<sub>14</sub>Ga<sub>10−m</sub>Al<sub>m</sub>Zn<sub>6</sub>O<sub>35</sub>:Dy<sup>3+</sup>,Mn<sup>4+</sup>(CGAZO:Dy<sup>3+</sup>,Mn<sup>4+</sup>) for plant growth LED lighting was successfully synthesized<italic>via</italic>high-temperature solid-state reaction method and an effective energy transfer from Dy<sup>3+</sup>to Mn<sup>4+</sup>in the CGAZO host had been verified.
Tanshinones are health-promoting diterpenoids found in Salvia miltiorrhiza and have wide applications. Here, SmGGPPS (geranylgeranyl diphosphate synthase) SmDXSII (1-deoxy-d-xylulose-5-phosphate were introduced into hairy roots of S. miltiorrhiza. Overexpression produces higher levels tanshinone than control single-gene transformed lines; production the double-gene line GDII10 reached 12.93 mg/g dry weight, which is highest content that has been achieved through genetic engineering....
Abstract Lantibiotics are a type of ribosomally synthesized and post‐translationally modified peptides (termed lanthipeptides) with often potent antimicrobial activity. Herein, we report the discovery new lantibiotic, lexapeptide, using library expression analysis system (LEXAS) approach. Lexapeptide has rare structural modifications, including N‐terminal ( N , )‐dimethyl phenylalanine, C‐terminal (2‐aminovinyl)‐3‐methyl‐cysteine, d ‐Ala. The characteristic lanthionine moiety in lexapeptide...
Hyperisampsins A–D (1–4), with tetracyclo[6.3.1.13,10.03,7]tridecane skeletons and seven biogenetically related congeners (5–11), were isolated from Hypericum sampsonii. Their structures elucidated by comprehensive spectroscopic techniques. The absolute configuration of 1 was established ECD calculations, those 5 9 confirmed single X-ray crystallographic analyses. A D showed potent anti-HIV activities EC50 2.97 0.97 μM selectivity index 4.80 7.70, respectively.
Pre-schisanartanin (1) and schindilactones A−C (2−4) were isolated from Schisandra chinensis. Among them, compound 1 had an unprecedented carbon skeleton. More importantly, the structure features of this provided new insight into biosynthesis triterpenoids with schisanartane Their structures determined by extensive spectroscopic analysis, single-crystal X-ray diffraction, on basis absolute one related nortriterpenoid (5), which was for first time a modified Mosher method.
ABSTRACT Streptomyces bingchenggensis is a soil-dwelling bacterium producing the commercially important anthelmintic macrolide milbemycins. Besides milbemycins, insecticidal polyether antibiotic nanchangmycin and some other antibiotics have also been isolated from this strain. Here we report complete genome sequence of S . The availability should enable us to understand biosynthesis these structurally intricate better facilitate rational improvement strain increase their titers.
Abstract Jasmonic acid (JA) is an important plant hormone involved in regulation of many aspects growth and development including secondary metabolism JASMONATE ZIM-DOMAIN (JAZ) proteins are key components JA signal processes. In this study, two new JAZ genes named SmJAZ3 SmJAZ9 were cloned from S. miltiorrhiza hairy roots characterized. Expression profiles under methyl jasmonate (MJ) treatment revealed that both MJ-responsive. Subcellular localization assay showed was located nucleus while...
Genome sequencing projects in the last decade revealed numerous cryptic biosynthetic pathways for unknown secondary metabolites microbes, revitalizing drug discovery from microbial by approaches called genome mining. In this work, we developed a heterologous expression and functional screening approach mining genomic bacterial artificial chromosome (BAC) libraries Streptomyces spp. We demonstrate strain of rochei, which is known to produce streptothricins borrelidin, expressing its BAC...
Some medicinal plants of the Solanaceae produce pharmaceutical tropane alkaloids (TAs), such as hyoscyamine and scopolamine. Littorine is a key biosynthetic intermediate in scopolamine pathways. However, mechanism underlying littorine formation from precursors phenyllactate tropine not completely understood. Here, we report elucidation biosynthesis through functional genomics approach identification two novel genes that encode UDP-glycosyltransferase (UGT1) synthase (LS). UGT1 LS are highly...
The tropane alkaloids (TAs) cocaine and hyoscyamine have been used medicinally for thousands of years. To understand the evolutionary origins trajectories serial biosynthetic enzymes TAs especially characteristic skeletons, we generated chromosome-level genome assemblies cocaine-producing Erythroxylum novogranatense (Erythroxylaceae, rosids clade) hyoscyamine-producing Anisodus acutangulus (Solanaceae, asterids clade). Comparative genomic phylogenetic analysis suggested that lack spermidine...
Rubriflordilactones A (1) and B (2), two novel highly unsaturated rearranged bisnortriterpenoids possessing a biosynthetically modified aromatic D-ring, were isolated from the leaves stems of Schisandra rubriflora. Their structures established on basis extensive spectroscopic methods, including two-dimensional NMR techniques, confirmed by X-ray crystallographic analysis. Compound 1 showed weak anti-HIV-1 activity, compound 2 exhibited an EC50 value 9.75 μg/mL (SI = 12.39) against HIV-1...
Lymphatic filariasis is caused by the parasitic nematodes Brugia malayi and Wuchereria bancrofti, asparaginyl-tRNA synthetase (AsnRS) considered an excellent antifilarial target. The discovery of three new tirandamycins (TAMs), TAM E (1), F (2), G (3), along with A (4) B (5), from Streptomyces sp. 17944 was reported. Remarkably, 5 selectively inhibits B. AsnRS efficiently kills adult parasite, representing a lead scaffold to discover develop drugs.
Abstract The pyridine ring is a potent pharmacophore in alkaloid natural products. Nonetheless, its biosynthetic pathways are poorly understood. Rubrolones A and B tropolone products possessing unique tetra-substituted moiety. Here, we report the gene cluster propose pathway for rubrolones, identifying key intermediate that accumulates upon inactivation of sugar genes. Critically, this was converted to aglycones rubrolones by non-enzymatic condensation cyclization with either ammonia or...
Rubrolones are tropolonoid natural products with a unique carbon skeleton. Extensive secondary metabolite analysis of the endophytic Streptomyces sp. KIB-H033 revealed new class rubrolone analogue possessing rare benzoic acid–pyridine inner salt moiety. Precursor feeding [13C]-acetate labeling pattern consistent tropolone moiety construction via type-II PKS chemistry followed by complex oxidative rearrangements. This bacterial biosynthetic route represents surprising departure from fungal...
Duclauxamide A1 (1), a new polyketide-derived heptacyclic oligophenalenone dimer with N-2-hydroxyethyl moiety, was isolated from Penicillium manginii YIM PH30375. Spectroscopic analysis, X-ray single crystal diffraction, and (13)C NMR DFT calculations confirmed that compound 1 other duclauxin analogues possess the unified S configuration at C-9', which corrects long-standing misrepresentation of duclauxins as C-9'R epimers. A plausible biosynthetic pathway for is proposed on basis previous...
The present study was conducted to examine the influence of a pathogen Sclerotinia sclerotiorum (Lib.) de Bary on actinobacterial community associated with soybean roots. A total 70 endophytic actinobacteria were isolated from surface-sterilized roots either healthy or diseased soybeans, and they distributed under 14 genera. Some rare genera, including Rhodococcus, Kribbella, Glycomyces, Saccharothrix, Streptosporangium Cellulosimicrobium, endemic samples, more diverse in samples compared...