A5100412213- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Microbial Metabolism and Applications
- Phytochemistry and Bioactivity Studies
- Chemical Synthesis and Analysis
- Genomics and Phylogenetic Studies
- Chemical synthesis and alkaloids
- Phytochemistry and Biological Activities
- Marine Sponges and Natural Products
- Biofuel production and bioconversion
- Synthetic Organic Chemistry Methods
- Plant-based Medicinal Research
- Bacterial biofilms and quorum sensing
- Fluorine in Organic Chemistry
- Pharmacological Effects of Natural Compounds
- Berberine and alkaloids research
- Antibiotics Pharmacokinetics and Efficacy
- Plant biochemistry and biosynthesis
- Synthesis and bioactivity of alkaloids
- Sulfur Compounds in Biology
- Sesquiterpenes and Asteraceae Studies
- Chemical Reactions and Isotopes
- Steroid Chemistry and Biochemistry
- Electrochemical Analysis and Applications
- Scoliosis diagnosis and treatment
First Affiliated Hospital of Henan University
2023-2024
Chinese Academy of Tropical Agricultural Sciences
2019-2023
Nanjing University
2023
Cornell University
2022
Second Affiliated Hospital of Zhengzhou University
2022
Hebei Medical University
2021
Third Hospital of Hebei Medical University
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Shanghai Jiao Tong University
2014-2020
Jiangsu Ocean University
2019
Abstract Lantibiotics are a type of ribosomally synthesized and post‐translationally modified peptides (termed lanthipeptides) with often potent antimicrobial activity. Herein, we report the discovery new lantibiotic, lexapeptide, using library expression analysis system (LEXAS) approach. Lexapeptide has rare structural modifications, including N‐terminal ( N , )‐dimethyl phenylalanine, C‐terminal (2‐aminovinyl)‐3‐methyl‐cysteine, d ‐Ala. The characteristic lanthionine moiety in lexapeptide...
Streptomyces lividans is a suitable host for the heterologous expression of biosynthetic gene clusters (BGCs) from actinomycetes to discover "cryptic" secondary metabolites. To improve BGCs, herein we optimized S. strain SBT5 via stepwise integration three global regulatory genes and two codon-optimized multi-drug efflux pump deletion negative gene, yielding four engineered strains. All optimization steps were observed promote production polyketides, non-ribosomal peptides, hybrid...
The roots of Berberis plants are widely used as a traditional Chinese medicine called "Sankezhen", having the activities antibacterial and anti-inflammatory, ingredients alkaloids. This work aims to study compare total alkaloids individual alkaloid (berberine) contents in stems from five origins (Berberis soulieana Schneid., B. henryana triacanthophora Fedde, gagnepainii Schneid. bergmanniae Schneid.) provides some references for resource quality evaluation medicine. Acid dye colorimetry...
Gallbladder cancer is the most common malignant tumor of biliary tract, and this condition has a rather dismal prognosis, with an extremely low five-year survival rate. To improve outcome unresectable recurrent gallbladder cancer, it necessary to develop new effective treatments drugs. The purpose present study was evaluate effects cordycepin on human cells uncover molecular mechanisms responsible for these effects. Cell Counting Kit-8 (CCK-8) colony formation assays revealed that affected...
Abstract Biobattery, a kind of enzymatic fuel cells, can convert organic compounds (e.g., glucose, starch) to electricity in closed system without moving parts. Inspired by natural starch metabolism catalyzed phosphorylase, isoamylase is essential debranch alpha-1,6-glycosidic bonds starch, yielding linear amylodextrin – the best for sugar-powered biobattery. However, there no thermostable stable enough simultaneous gelatinization and hydrolysis, different from case alpha-amylase. A putative...
Currently, hyperuricemia has shown a surprisingly rising trend, which attracts widespread attention due to potentially major health risks. Considering the inevitable side effects of long-term medicine, probiotics are emerging as potential therapeutics their ability improve uric acid metabolism and superior safety.In our study, two strains probiotics, Lactobacillus gasseri LG08 (LG08) Leuconostoc mesenteroides LM58 (LM58) isolated from kimchi were evaluated for prebiotic properties in vitro...
Five new perylenequinone derivatives, altertoxins VIII–XII (1–5), as well one known compound cladosporol I (6), were isolated from the fermentation broth of marine-derived fungus Cladosporium sp. KFD33 a blood cockle Haikou Bay, China. Their structures determined based on spectroscopic methods and ECD spectra analysis along with quantum calculations. Compounds 1–6 exhibited quorum sensing inhibitory activities against Chromobacterium violaceum CV026 MIC values 30, 20, 20 30 μg/well, respectively.
Wortmannolol (1), a new furanosteroid, along with five known compounds, wortmannolone (2), ergosterol (3), p-hydroxyphenyl ethanol (4), trans-6-dodecene (5), (2Z, 4E) -5-(8-hydroxy-1,5-dimethyl-3-oxo-6-oxabicyclo [3.2.1] octan-8-yl) -3-methylpenta-2,4-dienoic acid (6) were isolated from fungal endophyte Talaromyces sp. lgt-4. Their structures elucidated by IR, MS, 1D and 2D NMR spectra. Compound 1 show weak monoamine oxidase inhibitory activity.
Two new compounds, deacetylisowortmins A (1) and B (2), were isolated from Talaromyces wortmannii LGT-4. Their structures established by 1D 2D NMR spectra, as well comparison of the experimental calculated electronic circular dichroism spectra. Monoamine oxidase acetylcholinesterase inhibitory activities 1 2 also evaluated.
Abstract Lantibiotics are a type of ribosomally synthesized and post‐translationally modified peptides (termed lanthipeptides) with often potent antimicrobial activity. Herein, we report the discovery new lantibiotic, lexapeptide, using library expression analysis system (LEXAS) approach. Lexapeptide has rare structural modifications, including N‐terminal ( N , )‐dimethyl phenylalanine, C‐terminal (2‐aminovinyl)‐3‐methyl‐cysteine, d ‐Ala. The characteristic lanthionine moiety in lexapeptide...
[reaction: see text] An approach to the densely functionalized fluorocyclopropane 14, a key framework toward synthesis of mGluR 2 receptor agonist MGS0028 (1) is reported. The Trost AAA reaction enantioselectively introduced allylic stereogenic center and alpha-fluoroester moiety. Stereoselective epoxidation followed by intramolecular epoxide ring opening efficiently constructed 1-fluorocyclopropane-1-carboxylate matrix. This route can potentially be general methodology for concise, highly...
Nine pyrrole alkaloid derivatives, including four new ones (1-4), were isolated from the wild mushroom Lentinula edodes for first time. Their chemical structures determined using UV-Vis spectroscopy, IR MS, NMR and single-crystal X-ray diffraction techniques. Compound 1, a previously unreported bicylo-pyrrole aldehyde homologue, was found to be major component, approximately 8.2 μg g -1 in dry powder of L. edodes. 1 showed cytotoxicity against SMMC-772 (IC50 15.8 μM) without any cytotoxic...
Seven compounds were isolated from a marine-derived fungus Aspergillus sp. ZF-79, including three new polyketides (1-3), named asperochrins D-F, along with four known 4-7. Their structures determined on the basis of spectroscopic methods. All tested for quorum sensing inhibitory (QSI) activity. Compounds 1, 3, 4, 5, and 6 exhibited QSI activity against Chromobacterium violaceum CV026 MIC values 50, 100, 6.25 μM, respectively.
With the development and application of medical information system, semantic interoperability is essential for accurate advanced health-related computing electronic health record (EHR) sharing. The openEHR approach can improve interoperability. One key improvement that it allows use existing archetypes. crucial problem how to precision resolve ambiguity in archetype retrieval.Based on query expansion technology Word2Vec model Nature Language Processing (NLP), we propose find synonyms as...