A5090200155- Bacterial biofilms and quorum sensing
- Dental materials and restorations
- Legionella and Acanthamoeba research
- Antimicrobial Resistance in Staphylococcus
- Heme Oxygenase-1 and Carbon Monoxide
- Bone Tissue Engineering Materials
- Streptococcal Infections and Treatments
- Antifungal resistance and susceptibility
- Dental Implant Techniques and Outcomes
- Wound Healing and Treatments
- Bacterial Genetics and Biotechnology
- RNA regulation and disease
- Orthopedic Infections and Treatments
- Ocular Infections and Treatments
- Force Microscopy Techniques and Applications
- Forensic Toxicology and Drug Analysis
- Central Venous Catheters and Hemodialysis
- Calpain Protease Function and Regulation
- Oral microbiology and periodontitis research
- Advanced ceramic materials synthesis
- melanin and skin pigmentation
- Bacterial Infections and Vaccines
- Parasitic Infections and Diagnostics
Institute of Medical Microbiology and Hygiene
2019-2024
Saarland University
2019-2024
Candida albicans-related bloodstream infections are often associated with infected central venous catheters (CVC) triggered by microbial adhesion and biofilm formation. We utilized single-cell force spectroscopy (SCFS) flow chamber models to investigate the behavior of C. albicans yeast cells germinated naïve human blood plasma (HBP)-coated CVC tubing. Germinated demonstrated up 56.8-fold increased forces surfaces when compared cells. Coating CVCs HBP significantly 60-min but not 30-min...
The unicellular parasite Giardia duodenalis is the causative agent of giardiasis, a gastrointestinal disease with global spread. In its trophozoite form, G. can adhere to human intestinal epithelium and variety other, artificial surfaces. Its attachment facilitated by unique microtubule-based organelle, so-called ventral disc. mechanical function disc, however, still debated. Earlier studies postulated that dynamic negative pressure under generated persistently beating flagella, mediates...
Caries is one of the most prevalent diseases worldwide, which caused by degradation tooth enamel surface. In earlier research opportunistic pathogen Candida albicans has been associated with formation caries in children. Colonization teeth C. starts initial adhesion individual yeast cells to this study, we visualized colonization on pellicle-covered scanning electron microscopy. To quantitatively unravel strength, applied fluidic force microscopy-based single-cell spectroscopy examine key...
Abstract Invasion and persistence of bacteria within host cells requires that they adapt to life in an intracellular environment. This adaptation induces bacterial stress through events such as phagocytosis enhanced nutrient-restriction. During stress, synthesize a family proteins known heat shock (HSPs) facilitate survival. Previously, we determined the Staphylococcus aureus HSP ClpC temporally alters metabolism persistence. led us hypothesize might alter Inactivation clpC S. strain...
Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For of materials, particular attention has to be paid biocompatibility, osseointegration, and bacterial adhesion behavior. To understand their properties behavior, experimental studies with natural such as teeth are strongly required. The results, however, may highly case-dependent because surfaces have the disadvantage being subject...
Abstract Staphylococcus aureus is a common cause of catheter-related blood stream infections (CRBSI). The bacterium has the ability to form multilayered biofilms on implanted material, which usually requires removal medical device. A first major step this biofilm formation initial adhesion artificial surface. Here, we used single-cell force spectroscopy (SCFS) study S. central venous catheters (CVCs). SCFS performed with surfaces naïve CVCs produced comparable maximum forces three types in...
The purpose of this study was to analyze the concentration-dependent effects biguanides (polyhexamethylene biguanide [PHMB], chlorhexidine [CH]); diamidines (hexamidine-diisethionate [HD], propamidine-isethionate [PD], dibromopropamidine-diisethionate [DD]); natamycin (NM); miltefosine (MF); povidone iodine (PVPI), and chlorin e6 PDT on Acanthamoeba trophozoites cysts, in vitro.Strain 1BU cultured peptone-yeast extract-glucose medium. Trophozoites or cysts were PYG medium containing each...
We aimed to compare LDH release assay, trypan blue and fluorescent stainings, non-nutrient Escherichia coli plate assay in determining treatment efficacy of antiamoebic agents against Acanthamoeba castellanii trophozoites/cysts, vitro. 1BU trophozoites/cysts were challenged with 0.02% polyhexamethylene biguanid (PHMB), 0.1% propamidine isethionate (PD), 0.0065% miltefosine (MF). Efficacies the drugs determined by assays, Hoechst 33343, calcein-AM, ethidium homodimer-1 dyes, a agar E. assay....
ABSTRACT Dysfunctions in melanocytes can lead to pigmentation disorders, such as albinism, or contribute the development of melanoma, most aggressive form skin cancer. Epidermal typically interact with collagen IV-rich basement membrane, but upon injury pathological conditions, they encounter environments rich I fibronectin. While alterations ECM composition and stiffness are known impact cell behavior, specific roles each these cues for melanocyte functions remain unclear. To explore...
Abstract Research into materials for medical application draws inspiration from naturally occurring or synthesized surfaces, just like many other research directions. For of materials, particular attention has to be paid biocompatibility, osseointegration and bacterial adhesion behavior. To understand their properties behavior, experimental studies with natural such as teeth are strongly required. The results, however, may highly case-dependent because surfaces have the disadvantage being...
Bacterial biofilms formed on implanted medical devices pose a serious risk to the patient: Especially when inserted into blood system, such as central venous catheters (CVCs), this may yield in life-threatening infections endocarditis or sepsis [1].Along with exposure time within patient, CVCs are increasingly colonized by staphylococcal species S. aureus epidermidis [2].In past, many studies have investigated bacterial adhesion determining number of adherent cells per catheter segment, and...
Abstract Purpose To analyze the concentration dependent effect of diamidines (hexamidine‐diisethionat (HD), propamidin‐isethionate (PD), dibromopropamidine‐diisethionat (DD)); biguanides (polyhexamethylen biguanid (PHMB), chlorhexidine (CH)); natamycin (NM); miltefosine (MF); povidone iodine (PVPI) and chlorin e6 photodynamic therapy (PDT) on Acanthamoeba castellani trophozoites cysts, in vitro. Methods strain was cultured 712 peptone‐yeast extract‐glucose (PYG) medium. Thereafter, or cysts...
An amendment to this paper has been published and can be accessed via a link at the top of paper.