A5055153939- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Periodontal Regeneration and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Bone fractures and treatments
- Pluripotent Stem Cells Research
- Influenza Virus Research Studies
- Liver physiology and pathology
- Osteoarthritis Treatment and Mechanisms
- Biomedical Ethics and Regulation
- Nerve injury and regeneration
- Long-Term Effects of COVID-19
- Carcinogens and Genotoxicity Assessment
- Organ Transplantation Techniques and Outcomes
- Wound Healing and Treatments
- COVID-19 Clinical Research Studies
- Public Health and Nutrition
- CRISPR and Genetic Engineering
- Reconstructive Surgery and Microvascular Techniques
- Pancreatic function and diabetes
- Bone Tissue Engineering Materials
- DNA Repair Mechanisms
- Natural Products and Applications
- Phagocytosis and Immune Regulation
- 3D Printing in Biomedical Research
Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo
2015-2024
University of Indonesia
2015-2024
State University of Jakarta
2020
Indonesian Orthopaedic Association
2018
Stomatology Hospital
2017
Universitas Dr. Soetomo
2001
Osaka University
1987
Background. Stem cell-derived conditioned medium has a promising prospect to be produced as pharmaceuticals for regenerative medicine. Objective. To investigate various methods obtain stem (CM) get an insight into their of application in diseases. Methods. Systematic review using keywords “stem cell” and “conditioned medium” or “secretome” “therapy.” Data concerning treated conditions/diseases, type cell that was cultured, supplements culture the cells, condition, CM processing, growth...
Treatments for AGA have yet to produce satisfactory outcomes and may cause intolerable side effects. Recent studies reported that adipose tissue-derived stem cell conditioned media (ADSC-CM) could induce hair growth regeneration.To investigate the efficacy of ADSC-CM combined with minoxidil regeneration therapy in male AGA.This study lasted 6 weeks. Subjects were divided into two groups: concentrated non-concentrated ADSC-CM. Scalp was vertically half before intradermal injection...
Non-union due to large bone loss often causes significant long-term morbidity. We incorporate the use of allogeneic umbilical cord-derived mesenchymal stem cells (UC-MSCs) as part diamond concept regenerative medicine in a case infected non-union fracture. reported 54-year-old female patient presenting with pain on right thigh. She was previously diagnosed closed fracture femoral shaft and underwent four surgeries before finally being referred Dr. Cipto Mangunkusumo General Hospital shaft....
Background . Stromal vascular fraction (SVF) therapy has been performed over the past six years to treat 421 patients by our group in five clinical centers. Autologous SVF, which is a substance containing stem cells, was isolated from lipoaspirate, mixed with platelet-rich plasma (PRP), and administered degenerative diseases, autoimmune trauma, aging, other diseases unknown etiology. This study aimed determine safety of SVF PRP that were given through infusion, spinal, intra-articular...
Mesenchymal stem cells (MSCs), both endogenous and exogenous, enhance chondrocyte proliferation by stimulating collagen type II. Secretome, an MSC derivate, has shown to also provide this mechanism through a paracrine effect. We aimed evaluate the use of secretome in management early osteoarthritis (OA).19 (1 control) male sheep (Ovies aries), which were operated on with total lateral meniscectomy induce knee OA, divided into 3 groups: group, hyaluronic acid group. Each group was injected...
Stem cell culture for regenerative medicine needs platelet rich plasma (PRP) as fetal bovine/calf serum (FBS/FCS) substitute. However, the various studies used protocols in preparing and processing PRP. This study aimed to compare conclude most effective efficient protocol. Methods: we searched vitro that human PRP FBS/FCS substitute cells, compared available identify easiest preparation of release growth factors (GFs) support highest stem culture. Results: ten fulfilled selection criteria...
Adipose tissue derived mesenchymal stem cells (AT-MSCs) have similar properties to the previously characterized bone marrow (BM-MSCs). However, adipose is easier get in larger volumes, procedure less painful and at lower risks, yield more compared marrow. Moreover, considering various differentiation angiogenic potentials, ease collecting samples, immuno-suppressive properties, are very promising for regenerative medicine, therefore will be preferred source of MSCs future clinical use.
Th e hallmark of Parkinson's disease is on-going degeneration dopaminergic neurons in the substantia nigra, which may be due to various etiologies.Various approaches alleviate symptoms are available, such as life-long pharmacological intervention, deep brain stimulation, and transplantation neuron-containing fetal tissue.However, each these has a disadvantage.Several studies have shown that kinds stem cells, induced pluripotent other cells can diff erentiate into promising for treating...
Introduction: Hyperglycemia in diabetic patients induces elevated pro-inflammatory cytokine production, resulting cellular damage, which may affect the regenerative function of mesenchymal stem cells (MSCs), such as adipose-derived (ADSCs). Identifying effect diabetes on ADSCs and optimization culture conditions is therefore an important starting point for application autologous to improve clinicial outcomes. The aim this study was investigate that cultured low-glucose anti-oxidant-serum...
Platelet-rich plasma (PRP) contains pro-angiogenic growth factors including vascular endothelial factor (VEGF). Angiogenesis is a necessary component of wound healing in instances diabetic foot ulcers (DFU). PRP composition varies depending on methods and donor health status. Our group has developed an improved protocol for diabetes treatment. The aims this study were to examine the levels VEGF these patient populations with without diabetes.PRP was prepared using 24 mL whole blood from 13...
Background: Lipoaspirate contains noxious substances derived from liposuction. Therefore, extensive washing is recommended before the lipoaspirate processed further for culture or fat grafting. Washing a small amount of may not pose problem, but large volume be cumbersome, time consuming, and requires lot phosphate buffered saline (PBS). Objective: To introduce simple method using fine-mesh stainless-steel tea coffee filter, spoon, porcelain bowl. Methods: The filter was used to collect...
Background: Lipoaspirate-derived stem cells (LSCs) are very promising for regenerative medicine, e.g. to treat acute myocard infarction. Fetal bovine serum (FBS) is commonly used propagate the LSCs. However, its clinical application, FBS contains xeno-proteins that potential elicit immune rejection in patients. Platelet rich plasma (PRP) one of candidates replace FBS. This study was aimed compare proliferation LSCs cultured 5% PRP, 10% and containing medium (MesenCult®). Methods: were...
Recent studies have shown that human bone marrow-derived mesenchymal stem cells (hBM-MSCs) several drawbacks in treating critical-sized defect (CSD). Secretome may offer considerable advantages over living terms of potency, manufacturing and storing easiness, potential as a ready-to-go osteoinductive agent. However, thus far, there are no regarding the efficacy secretome healing. The objective this study is to investigate effect rat models with CSD.This was an experimental post-test only...