A5061587223- Advanced Fluorescence Microscopy Techniques
- Near-Field Optical Microscopy
- Plasmonic and Surface Plasmon Research
- Advanced Electron Microscopy Techniques and Applications
- Optical Imaging and Spectroscopy Techniques
- Advanced Optical Sensing Technologies
- Particle Accelerators and Free-Electron Lasers
- Advanced X-ray and CT Imaging
- Medical Imaging Techniques and Applications
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Force Microscopy Techniques and Applications
- Experimental and Theoretical Physics Studies
- Cellular Mechanics and Interactions
- Photonic and Optical Devices
- Silk-based biomaterials and applications
- Quantum and Classical Electrodynamics
- Luminescence Properties of Advanced Materials
- Characterization and Applications of Magnetic Nanoparticles
- Protein Interaction Studies and Fluorescence Analysis
- Electrochemical Analysis and Applications
- Nanofabrication and Lithography Techniques
- Advanced biosensing and bioanalysis techniques
- Molecular Junctions and Nanostructures
- Cancer Cells and Metastasis
University of Göttingen
2014-2020
Czech Academy of Sciences, Institute of Physics
2019-2020
We present a comprehensive theory of dead-time effects on Time-Correlated Single Photon Counting (TCSPC) as used for fluorescence lifetime measurements, and develop correction algorithm to remove these artifacts. apply this measurements well Fluorescence Lifetime Imaging Microscopy (FLIM), where rapid data acquisition is necessarily connected with high count rates. There, cannot be neglected, lead distortions in the observed image. The quite general completely independent particular nature...
Metallic nanoparticles were shown to affect Förster energy transfer between fluorophore pairs. However, date, the net plasmonic effect on FRET is still under dispute, with experiments showing efficiency enhancement and reduction. This controversy due challenges involved in precise positioning of pairs near field a metallic nanostructure, as well accurate characterization impact mechanism. Here, we use DNA origami technique place pair 10 nm away from surface gold sizes ranging 5 20 nm. In...
The biological process of the epithelial-to-mesenchymal transition (EMT) allows epithelial cells to enhance their migratory and invasive behavior plays a key role in embryogenesis, fibrosis, wound healing, metastasis. Among multiple biochemical changes from an mesenchymal phenotype, alteration cellular dynamics cell–cell as well cell–substrate contacts is crucial. To determine these variations over whole time scale EMT, we measure distance NMuMG during EMT using our newly established...
Single-molecule localization based super-resolution microscopy has revolutionized optical and routinely allows for resolving structural details down to a few nanometers. However, there exists rather large discrepancy between lateral axial accuracy, the latter typically three five times worse than former. Here, we use single-molecule metal-induced energy transfer (smMIET) localize single molecules along axis, measure their distance with an accuracy of 5 nm. smMIET relies only on fluorescence...
The nuclear envelope, comprising the inner and outer membrane, separates nucleus from cytoplasm plays a key role in cellular functions. Nuclear pore complexes (NPCs), which are embedded control transport of macromolecules between two compartments. Here, using dual-color metal-induced energy transfer (MIET), we determine axial distance Lap2β Nup358 as markers for membrane cytoplasmic side NPC, respectively. Using MIET imaging, reconstruct 3D profile envelope over whole basal area, with an...
Our paper presents the first theoretical and experimental study using single-molecule Metal-Induced Energy Transfer (smMIET) for localizing single fluorescent molecules in three dimensions. describes resonant energy transfer from excited state of a emitter to surface plasmons metal nanostructure. This is strongly distance-dependent can be used localize an along one dimension. We have past emitters with nanometer accuracy optical axis microscope. The combination smMIET localization based...
We report a novel method, dual-color axial nanometric localization by metal-induced energy transfer, and combine it with Förster resonance transfer (FRET) for resolving structural details in cells on the molecular level. demonstrate capability of this method cytoskeletal elements adhesions human mesenchymal stem cells. Our approach is based fluorescence-lifetime-imaging microscopy allows precise determination three-dimensional architecture stress fibers anchoring at focal adhesions, thus...
Photobleaching of fluorophores is one the key problems in fluorescence microscopy. Overcoming limitation maximum number photons, which can be detected from a single emitter, would allow to enhance signal-to-noise ratio and thus temporal spatial resolution imaging. It breakthrough for many applications spectroscopy, are unachievable up now. So far, only approach diminishing effect photobleaching has been photostability an emitter. Here, we present fundamentally new solution increasing photons...
Abstract One of the key photophysical properties fluorescent proteins that is most difficult to measure quantum yield. It describes how efficiently a fluorophore converts absorbed light into fluorescence. Its measurement using conventional methods become particularly problematic when it unknown many proposedly molecules sample are indeed (for example due incomplete maturation, or presence dark states). Here, we use plasmonic nanocavity-based method absolute yield values commonly used...
Metal-induced electron transfer imaging is employed to study the adhesion of human blood platelets in a time-resolved manner.
Precise knowledge of the quantum yield is important for many fluorescence–spectroscopic techniques, example, Förster resonance energy transfer. However, to measure it emitters in a complex environment and at low concentrations far from being trivial. Using plasmonic nanocavity, we absolute value lipid-conjugated dyes incorporated into supported lipid bilayer. We show that both hydrophobic hydrophilic molecules inside bilayer strongly differs its aqueous solution. This finding particular...
Generation of electromagnetic fields by moving charges is a fascinating topic where the tight connection between classical electrodynamics and special relativity becomes particularly apparent. One can gain direct insight into structure such visualizing electric field lines. However, calculation lines for arbitrarily far from trivial. Here, we derive an equation direction that points retarded position charge towards specific line position, which allows simple construction these We...
Fluorescence lifetime microscopy has become an important method of bioimaging, allowing not only to record intensity and spectral, but also information across image. One the most widely used methods FLIM is based on Time-Correlated Single Photon Counting (TCSPC). In TCSPC, one determines this curve by exciting molecules with a periodic train short laser pulses, then measuring time delay between first recorded fluorescence photon after each pulse. An technical detail TCSPC measurements fact...
Generation of electromagnetic fields by moving charges is a fascinating topic where the tight connection between classical electrodynamics and special relativity becomes particularly apparent. One can gain direct insight into structure such visualizing electric field lines. However, calculation lines for arbitrarily far from trivial. Here, we derive an equation director that points retarded position charge towards specific line position, which allows simple construction these We analytically...