A5008358264- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Muscle Physiology and Disorders
- Extracellular vesicles in disease
- Cellular transport and secretion
- CAR-T cell therapy research
Cincinnati Children's Hospital Medical Center
2022-2023
Muscle cell fusion is a multistep process where the final step of reaction drives progression beyond early hemifusion events to complete fusion. This requires activity muscle-specific fusogen Myomerger, single-pass transmembrane protein containing 84 amino acids with an ectodomain that includes two α-helices. Previous studies have demonstrated Myomerger acts by destabilizing membranes through generation elastic stresses in outer leaflet plasma membrane. An obvious question how such might be...
Entry of enveloped viruses into cells is mediated by fusogenic proteins that form a complex between membranes to drive rearrangements needed for fusion. Skeletal muscle development also requires membrane fusion events progenitor multinucleated myofibers. Myomaker and Myomerger are muscle-specific cell fusogens, but do not structurally or functionally resemble classical viral fusogens. We asked if the fusogens could substitute despite their structural distinctiveness, fuse cells. report...