Abstract Two phylogenetically distant types of test‐systems—root tip meristems barley ( Hordeum vulgare ) and human lymphocytes in vitro were used to detect genotoxicity cytotoxicity induced by the herbicide paraquat (PQ) concentration range (10 −6 5 × 10 −4 mol/l). As an endpoint for mitotic index (MI) was evaluated. The frequency chromosome aberrations (CA) micronuclei (MN) as endpoints genotoxicity. A dose‐dependent increase CA MN observed both test systems, although values PQ‐induced...

The steam distillation of valuable rose essential oil from R. damascena Mill. and alba L. generates large volumes wastewaters. Although such wastewaters are bio-pollutants, they contain bioactive compounds. In this study we investigated the cytotoxic/genotoxic anti-cytotoxic/anti-genotoxic potential these products. We used cytogenetic methods for induction chromosome aberrations micronuclei in two different experimental test-systems: ahigher plant human lymphocyte cultures. Different schemes...

Rosa centifolia L. and gallica (Rosaceae) are grown as raw materials for valuable essential oils hydrosols. There scarce data about the biological activities genoprotective potential of hydrosols these roses. The aim study was to provide information on their cytotoxic/genotoxic activity anti-cytotoxic/anti-genotoxic capacity against mutagenic N-methyl-N′-nitro-N-nitrosoguanidine (MNNG). evaluation performed using classical tests chromosomal aberrations micronuclei in higher plant Hordeum...

Geraniol is a common component of essential oils various plant species and widely used in cosmetics, perfumery household products. In the present study, we investigated cytotoxic, genotoxic antioxidant activities different concentrations monoterpenoid geraniol its anti-cytotoxic anti-genotoxic potential against genotoxin N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) higher human lymphocyte test-systems frequently genotoxicity studies. The cytogenetic analysis did not show any inhibition cell...

Organisms are constantly exposed to the detrimental effect of environmental DNA-damaging agents. The harmful effects genotoxins could be decreased in a viable way by antimutagenesis. One modern approaches reduce mutagenic burden is based on exogenous natural and synthetic compounds that possess protective antimutagenic potential against genotoxins. kaempferol jatropham isolated from Lilium candidum were tested with respect their protect cells radiomimetic zeocin, as well cytotoxic genotoxic...

The Rosa alba L. and damascena Mill. growing in Bulgaria are known for their extremely fine essential oil valuable hydrosols. Irrespectively of its wide use human life, little research exists on the cytotoxic genotoxic activity This set our goal to conduct cytogenetic analyses study these effects. A complex classical methods was applied three types experimental test systems-higher plant vivo, ICR mice lymphocytes vitro. Mitotic index, PCE/(PCE + NCE) ratio, nuclear division index were used...

Abstract This work evaluated the ability of one synthetic compound 1‐(4‐fluorophenylthiocarbamoyl)‐4‐methyl‐piperazine (FTMP), thiourea derivative to reduce cytoxic and genotoxic effects free radical inducer paraquat (PQ) in two different test systems Hordeum vulgare human lymphocytes vitro . The mitotic index was used as a marker for cytotoxicity. To indicate genotoxicity, chromosome aberrations micronucleus induction were used. FTMP manifested weak effect both systems. Clear evidence...

Plants in natural ecosystems are exposed to a combination of UV radiation, ionizing radiation (IR) and other abiotic factors. These factors change with the altitude. We investigated DNA alterations some wild plants different plant families at three altitudes Rila Mountain, Bulgaria (1500, 1782, 2925 m above sea level (a.s.l.) IR stresses, assess tolerance species changing environmental conditions successive growth seasons. For this purpose, physicochemical, cytogenetic, molecular methods...

The highly valued oil of Rosa damascena Mill. (Rosaceae), widely used in high perfumery, cosmetics, and other spheres human life, obliges us to know study the safety profile product obtained from water–steam distillation fresh rose petals. genotoxicity essential (EsO) has not been thoroughly studied despite its wide range applications. That predetermined object this study—to evaluate, through classical cytogenetic methods, possible cytotoxic/genotoxic activities R. EsO (EsORdm) three...

Bulgarian Rosa damascena Mill. is has been known since ancient times for its high-quality oil, hydrosol, and other aromatic products. Rose hydrosol various biological activities, but no research on anticytotoxic/antigenotoxic effects exists. This study aimed to assess defense potential against the genotoxin N-methyl-N′-nitro-N-nitrosoguanidine test cytotoxic/genotoxic activity in plant human lymphocyte systems. Endpoints cytotoxicity (mitotic index nuclear division index) genotoxicity...

The aim of this study is to assess the potential prolonged UV irradiation induce genotoxic alteration in Poaceae species cultivated laboratory and mountain conditions.Changes natural environment increase a great extent with altitude.In ecosystems plants are exposed other environmental factors for more than one period time 10, 20, 30 or 43 days.Four wild species: Poa alpina L., Sesleria coerulans Friv., Festuca valida (R. Uechtr.)Pénzes, Dactylis glomerata characteristic Rila Mountain at...

In this paper we aimed to evaluate the cytotoxic, genotoxic and modulating potential of algal supernatants isolated from untreated cell suspension—SN(-), suspension treated with CdCl2 10−6, 10−5 10−4 mol/l (SN), supplemented various concentrations CdCl2—10−6, mol/l—SN(+). All data were compared cytotoxic effects single cadmium treatment.Direct mutagenic assay Cell-free medium/Lymphocytes performed. Cell-wall-less strain cw15 Chlamydomonas reinhardtii was grown for further procedures. Human...

Rila Mountain is the highest mountain on Balkan Peninsula and characterized with specific microclimate.It has been revealed that increase of altitude, differences in environmental conditions change at a great extent.In plants have to cope combined factors such as temperature, prolonged UV irradiation, etc.The aim this study assess whether pigment content wild growing plant species altitudes dependent.Five species, characteristic ecosystems Mountain: Fragaria vesca L. (Rosaceae), Myosotis...