A5029433347- Protein Hydrolysis and Bioactive Peptides
- Insect Utilization and Effects
- Ginger and Zingiberaceae research
- Algal biology and biofuel production
- Curcumin's Biomedical Applications
- Fungal Biology and Applications
- Aquaculture Nutrition and Growth
- Biofuel production and bioconversion
- Meat and Animal Product Quality
- Microbial Metabolic Engineering and Bioproduction
- Pharmacological Effects of Natural Compounds
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- melanin and skin pigmentation
- Phytochemicals and Antioxidant Activities
- Biochemical effects in animals
- Biochemical Analysis and Sensing Techniques
- Bee Products Chemical Analysis
- Genomics, phytochemicals, and oxidative stress
- Microbial Metabolites in Food Biotechnology
- Coffee research and impacts
- Natural Antidiabetic Agents Studies
- Seaweed-derived Bioactive Compounds
- Fungal and yeast genetics research
- Polysaccharides Composition and Applications
Chulalongkorn University
2015-2024
Czech Academy of Sciences, Institute of Biotechnology
2019-2021
Bangkok University
2019-2021
Biocon (Switzerland)
2019
The polysaccharides found in Caulerpa lentillifera (sea grape algae) are potentially an important bioactive resource. This study makes use of RSM (response surface methodology) to determine the optimal conditions for extraction valuable SGP polysaccharides). findings indicated that a water/raw material ratio 10:1 mL/g, temperature 90 °C, and time 45 min would maximize yield, with experimentation achieving yield 21.576 %. After undergoing purification through DEAE-52 cellulose Sephacryl S-100...
An extracellular β-glucosidase was purified from culture filtrates of the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm grown on 1.0% (w/v) carboxymethyl-cellulose using ammonium sulfate precipitation, ion-exchange, hydrophobic interaction and gel filtration chromatography. The enzyme is monomeric with a molecular weight 64.2 kDa as estimated by sodium dodecyl sulfate-polyacrylamide electrophoresis, has pI 8.55. catalyzes hydrolysis p-nitrophenyl-β-d-glucopyranoside (PNPG)...
The study determines optimized process conditions to maximize ACE inhibitory peptide production. two novel hexa-peptides (LGRNLPPI and GPAGPAGL) from de-fatted lemon basil seeds (DLBS) was achieved.
Defatted rice bran (DRB) is a by-product of oil extraction from (Oryza sativa L.), and has health benefits. This in vitro study investigated the antioxidant anti-inflammatory effects four crude DRB protein hydrolysate fractions prepared by pepsin-pancreatin digestion fractionated ultrafiltration on transformed macrophage cell line, RAW264.7. Protein hydrolysates with MW <3 kDa (F4 fraction) had lowest IC50 for NO-, DPPH˙ ABTS radical scavenging activities (0.38 ± 0.01, 1.24 0.03 0.17 0.01...
The Synechococcus sp. VDW peptides were prepared by trypsin digestion, which was then purified ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. Among the fractions, MW <3 kDa fraction exhibited high levels inhibitory activity toward NO radical scavenging activities IC50 values 34.51 ± 9.8 μg/mL. reversed-phase HPLC to yield four fractions. 30–40 min sub-fraction maximum (77.50 0.55%) selected for further analysis Q-TOF ESI mass spectrometry. Five isolated amino...
Angiotensin converting enzyme (ACE) inhibition offers a useful means of managing hypertension, because ACE inhibitors (ACEIs) are known to serve as agents with antihypertensive properties in addition generating positive metabolic and cardioprotective outcomes. However, current ACEIs linked adverse consequences, so there is requirement for effective but safer compounds, which might be achieved through chemical synthesis or the isolation naturally obtained bioactive molecules. Protein...
This research examined the antioxidant abilities of peptides derived from <italic>Hericium erinaceus</italic> mushroom produced <italic>via</italic> three microbial proteases at varying concentrations.
Abstract It is anticipated that calcium-chelating peptides may serve to enhance the absorption of calcium. This research examined defatted lemon basil seeds (DLBS) which had been treated with Alcalase under optimized parameters for degree hydrolysis proteolysis, discovering activity calcium-binding in a competitive condition phosphate ion was 60.39 ± 1.545%. The purification hydrolysates performed via ultrafiltration along reversed-phase high performance liquid chromatography (RP-HPLC)....
The lingzhi mushroom (Ganoderma lucidum) is well known for its medicinal properties and has long played a role in traditional oriental medicine due to health-giving benefits potential extend life expectancy. contains number of highly bioactive compounds can also act as an excellent source protein. This research investigated the peptides obtained from protein hydrolysates mushrooms assess their free radical scavenging abilities. These were acquired via different proteases (Alcalase, Neutrase,...
The purpose of this study is to assess the bioactive peptides derived from defatted lemon basil seeds hydrolysate (DLSH) for their ability inhibit pancreatic lipase, decrease intracellular lipid accumulation, and reduce adipogenesis. Response surface methodology (RSM) was employed optimize trypsin hydrolysis conditions maximizing lipase inhibitory activity (LI). A time 387.06 min, a temperature 49.03°C, an enzyme concentration 1.61% w/v, resulted in highest LI with IC 50 368.07 μg/mL....
Rambutan seeds are by-products generated from fruit-processing factories; the leftover buried in landfills, generating methane emissions. This work aimed to extract polysaccharides (POLS) rambutan by using subcritical water extraction (SWE). The effects of defatting pretreatment and operating parameters SWE were investigated a Box-Behnken design. results show that significantly enriched POLS yield, while it had no significant effect on total sugar content. Using desirability approach,...
Peanut worm (Sipunculus nudus Linn.) protein was hydrolyzed by three proteases, and NO scavenging activity of the hydrolysates evaluated. The hydrolysate obtained using Alcalase® showed highest activity. This fractionated 10-, 5-, 3 kDa molecular weight cut-off membranes, lowest MW fraction (<3 kDa) exhibited <3 further purified gel filtration high-performance liquid chromatographies. peptides in HPLC with strongest anti-NO were identified quadrupole-time-of-flight mass spectrometry as...
Salmon bones, a waste by-product from the salmon industry, were used as protein hydrolysate source for production of bioactive peptides. The aim this work was to evaluate potential antioxidant and anti-inflammatory properties bone (SBPH).Salmon bones hydrolyzed by separately using one four proteases (Alcalase, Favourzyme, Neutrase papain) at various concentrations (10, 25 50 mg mL-1 ), where SBPH derived 10 papain hydrolysis exhibited highest nitric oxide (NO) radical scavenging activity....
Bee pollen protein was hydrolyzed using the commercial Alcalase, Flavourzyme and Neutrase enzymes. The hydrolysate formed from a 1:1 (v/v) enzyme/substrate ratio (NH1) showed highest nitric oxide (NO) radical scavenging activity. NH1 further separated into five fractions based on molecular weight (MW1–5) MW1, smallest fraction (< 0.65 kDa), possessed NO inhibitory effects of production were assessed by incubating with lipopolysaccharide-stimulated RAW264.7 macrophage cells. levels culture...
De-oiled rice bran (DORB) is a potentially useful by-product of the oil industry. DORB may prove to be an important protein source, and also contains many other micronutrients. This study has principal aim optimizing process hydrolysate preparation, then testing determine its lipase inhibitory activity. underwent hydrolysis using Alcalase® response surface methodology (RSM). The resulting degree (DH) was monitored along with extent any optimum levels inhibition were obtained at temperature...
This study aims to determine the antioxidant activity of bioactive peptides derived from Synechococcus sp. VDW cells cultured for 21 days. protein hydrolysates were prepared with trypsin and purified by ultrafiltration molecular mass cut-off membranes 10, 5 3 kDa. The M<3 kDa (FA) fraction had highest 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, IC50 values (11.5±0.3) (13.6±0.2) µg/mL, respectively. FA was...