- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Metalloenzymes and iron-sulfur proteins
- Microbial Fuel Cells and Bioremediation
- Antibiotic Resistance in Bacteria
- Protein Hydrolysis and Bioactive Peptides
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Aquaculture Nutrition and Growth
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Light effects on plants
- Global Energy and Sustainability Research
- Biodiesel Production and Applications
- Biocrusts and Microbial Ecology
- Meat and Animal Product Quality
- Nanocomposite Films for Food Packaging
- Bacterial Genetics and Biotechnology
- Seaweed-derived Bioactive Compounds
- Photochromic and Fluorescence Chemistry
- Insect Utilization and Effects
- Bacterial biofilms and quorum sensing
King Mongkut's Institute of Technology Ladkrabang
2014-2025
Philipps University of Marburg
1996-2007
Kiel University
2005
Summary The bidirectional NiFe‐hydrogenase of Synechocystis sp. PCC 6803 is encoded by five genes ( hoxEFUYH ) which are transcribed as one unit. transcription the hox ‐operon regulated a promoter situated upstream hoxE . start point was located at −168 5′Race. Several probe vectors carrying different fragments revealed two regions to be essential for activity. One in untranslated 5′leader region and other found −569 −690 nucleotides ATG. further shown bind protein. Even though an imperfect...
The emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant (XDR-TB) makes the treatment control difficult. Rapid detection strains is important for successful tuberculosis; however, not all resistance mechanisms to injectable second-line drugs such as amikacin (AK), kanamycin (KM), capreomycin (CAP) are well understood. This study aims validate associated with AK, KM, CAP in M. clinical isolated Thailand. A total 15,124 were from 23,693 smear-positive sputum...
The Synechococcus sp. VDW peptides were prepared by trypsin digestion, which was then purified ultrafiltration with molecular weight cut off membranes of 10, 5 and 3 kDa. Among the fractions, MW <3 kDa fraction exhibited high levels inhibitory activity toward NO radical scavenging activities IC50 values 34.51 ± 9.8 μg/mL. reversed-phase HPLC to yield four fractions. 30–40 min sub-fraction maximum (77.50 0.55%) selected for further analysis Q-TOF ESI mass spectrometry. Five isolated amino...
Hydrogen (H2) is a promising clean energy carrier that can be produced from green algae. By screening H2-producing Chlorella species, sp. ChiW1, isolated rice paddy field in Chai Nat province, Thailand, exhibited the highest H2 production rate and yield. The mid-logarithmic phase cells of ChiW1 demonstrated production. Nitrogen deprivation doubled compared to normal conditions, due increased hydrogenase activity resulting reduced O2 evolution. Furthermore, high concentration acetic acid...
Light characteristics, including spectrum and intensity, significantly impact cyanobacterial biomass production, pigment biosynthesis, cellular metabolism, influencing the composition of various biochemical compounds. This study aimed to investigate effects light-emitting diode (LED) illumination on biomass, pigment, lipid production in unicellular halotolerant cyanobacterium Aphanothece halophytica, cultivated a suitable natural seawater (SNSW) medium. The results revealed that LED light...
In this paper, titanium dioxide nanosheets (Ti0.91O2 NSs) were incorporated into bacterial cellulose (BC) film for dielectric property tuning while maintaining the flexibility of resulting composite paper. By taking advantage improved constant, nanosheets/BC composites employed as capacitive sensors. The fabricated devices showed highest sensing performance ∼2.44 × 10-3 kPa-1 from 0 to 30 N when incorporating little 3 vol% Ti0.91O2 NSs (or ∼2 wt% Ti). Stable operation and high robustness...
The unicellular halotolerant cyanobacterium Aphanothece halophytica is a potential dark fermentative producer of molecular hydrogen (H 2 ) that produces very little H under illumination. One factor limiting the photoproduction this an inhibition bidirectional hydrogenase activity by oxygen (O obtained from splitting water molecules via photosystem II activity. present study aimed to investigate effects inhibitors carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and...
This study aims to determine the antioxidant activity of bioactive peptides derived from Synechococcus sp. VDW cells cultured for 21 days. protein hydrolysates were prepared with trypsin and purified by ultrafiltration molecular mass cut-off membranes 10, 5 3 kDa. The M<3 kDa (FA) fraction had highest 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities, IC50 values (11.5±0.3) (13.6±0.2) µg/mL, respectively. FA was...
Aminoglycosides such as amikacin and kanamycin are effective injectable second-line drugs for treatment of multidrug-resistant tuberculosis. Molecular mechanisms underlying aminoglycoside resistance not well understood. We have previously identified the amikacin- kanamycin-resistant M. tuberculosis MT433 clinical strain, which all known mutations related to been found. Drug efflux pump is one reported that might play a role in resistance.The expression levels sixteen putative genes,...
In N2-fixing cyanobacteria, three enzymes are involved in the H2 metabolism. Nitrogenase catalyzes N2 fixation which produces as a by-product. The produced is taken up to protons and electrons by an activity of uptake hydrogenase. Reversible enzyme both reactions evolution uptake. These all metalloenzyme. cyanobacterial nitrogenase normally requires molybdenum iron cofactors; however few species dependent on vanadium. reversible hydrogenase nickel cofactors. This research aimed investigate...
H2 produced by cyanobacteria is one of the interesting alternative energy carriers for future. The filamentous N2-fixing cyanobacterium Anabaena siamensis TISTR 8012 a potential microorganism production. It can produce via both photosynthesis and nitrogen fixation processes. This study aimed to increase efficiency production immobilization A. cells. result showed that rate grown in BG110 medium was higher than BG11 Allen-Arnon media due an heterocyst sulfur deprivation during adaptation...
The green alga Tetraspora sp. CU2551 has previously been identified as a hydrogen producing organism. With the promising potential in high H2 production yield, we further studied enhancement of capacity by substrate-level control to key enzyme, Hydrogenase. This enzyme catalyzes formation biohydrogen gas from protons and electrons. Here, investigated effect pH on rate which directly affected flow proton flux hydrogenase enzyme. result revealed that culture TAP medium was simply controlled...
To investigate the potential of application marine cyanobacterium for concurrent biomass production and ammonium removal, Synechococcus sp. VDW was cultured under different conditions in medium containing varying concentrations NH4Cl. Response surface methodology (RSM) then used to build a predictive model combined effects independent variables (pH, inoculum size, concentration). At optimum initial pH 7.4, size 0.17 (OD730) concentration 10.5 mg L-1, maximum removal productivity were about...