Kieran Hyslop
A5089157012- Transplantation: Methods and Outcomes
- Organ Transplantation Techniques and Outcomes
- Mechanical Circulatory Support Devices
- Respiratory Support and Mechanisms
- Immune Response and Inflammation
- Cardiac Ischemia and Reperfusion
- Sepsis Diagnosis and Treatment
- Cardiac Arrest and Resuscitation
- Thermal Regulation in Medicine
- Neonatal Respiratory Health Research
- Renal Transplantation Outcomes and Treatments
- Hemodynamic Monitoring and Therapy
- Neuroinflammation and Neurodegeneration Mechanisms
- Cardiovascular Function and Risk Factors
- Aortic Disease and Treatment Approaches
- Genetic and phenotypic traits in livestock
- Fuel Cells and Related Materials
- Cardiac Arrhythmias and Treatments
- Bacterial Identification and Susceptibility Testing
- Reproductive Physiology in Livestock
- Cardiac Structural Anomalies and Repair
- Reproductive Biology and Fertility
- Ultrasound in Clinical Applications
- Neurological Complications and Syndromes
- Delphi Technique in Research
Prince Charles Hospital
2020-2024
The University of Queensland
2019-2024
Holy Spirit Northside Private Hospital
2022
Collaborative Research Group
2022
AgResearch
2020
The global shortage of donor hearts available for transplantation is a major problem the treatment end-stage heart failure. ischemic time using traditional preservation by standard static cold storage (SCS) limited to approximately 4 hours, beyond which risk primary graft dysfunction (PGD) significantly increases. Hypothermic machine perfusion (HMP) has been proposed safely extend without increasing PGD.Using our sheep model 24 hours brain death (BD) followed orthotopic (HTx), we examined...
The acute respiratory distress syndrome (ARDS) describes a heterogenous population of patients with severe failure. However, contemporary advances have begun to identify distinct sub-phenotypes that exist within its broader envelope. These varied outcomes and respond differently several previously studied interventions. A more precise understanding their pathobiology an ability prospectively them, may allow for the development precision therapies in ARDS. Historically, animal models played...
Mortality and morbidity of Acute Respiratory Distress Syndrome (ARDS) are largely unaltered. A possible new approach to treatment ARDS is offered by the discovery inflammatory subphenotypes. In an ovine model phenotypes, matching key features human subphenotypes, we provide imaging characterization using computer tomography (CT). Nine animals were randomized into (a) OA (oleic acid, hypoinflammatory; n = 5) (b) OA-LPS acid lipopolysaccharides, hyperinflammatory; 4). 48 h after induction...
Abstract Differential hypoxaemia (DH) is common in patients supported by femoral veno-arterial extracorporeal membrane oxygenation (V-A ECMO) and can cause cerebral hypoxaemia. To date, no models have studied the direct impact of flow on damage. We investigated V-A ECMO brain injury an ovine model DH. After inducing severe cardiorespiratory failure providing support, we randomised six sheep into two groups: low (LF) which was set at 2.5 L min −1 ensuring that entirely perfused native heart...
Left ventricular stroke work index (LVSWI) and afterload-related cardiac performance (ACP) consider left (LV) afterload could be better prognosticators in septic cardiomyopathy. However, their invasive nature prevents routine clinical applications. This study aimed to investigate (1) whether a proposed speckle-tracking echocardiography parameter, Pressure-Strain Product (PSP), can non-invasively predict catheter-based LVSWI, ACP serum lactate an ovine model of cardiomyopathy; (2) PSP...
Whilst the presence of 2 subphenotypes among heterogenous Acute Respiratory Distress Syndrome (ARDS) population is becoming clinically accepted, subphenotype-specific treatment efficacy has yet to be prospectively tested. We investigated anti-inflammatory in different ARDS models sheep, previously shown similarities human subphenotypes, a preclinical, randomized, blinded study. Thirty anesthetized sheep were studied up 48 h and randomized into: (a) OA: oleic acid (n = 15) (b) OA-LPS:...
Abstract Background A lung transplant is the last resort treatment for many patients with advanced disease. The majority of donated lungs come from donors following brain death (BD). endothelin axis upregulated in blood and donor after BD resulting systemic inflammation, damage poor graft outcomes recipient. Tezosentan (endothelin receptor blocker) improves pulmonary haemodynamic profile; however, it induces adverse effects on other organs at high doses. Application ex vivo perfusion (EVLP)...
Organs procured following brain stem death (BSD) are the main source of organ grafts for transplantation. However, BSD is associated with inflammatory responses that may damage and affect both quantity quality organs available transplant. Therefore, we aimed to investigate plasma bronchoalveolar lavage (BAL) pro-inflammatory cytokine profiles cardiovascular physiology in a clinically relevant 6-h ovine model BSD.Twelve healthy female sheep (37-42 Kg) were anaesthetized mechanically...
Heart transplantation (HTx) from brainstem dead (BSD) donors is the gold-standard therapy for severe/end-stage cardiac disease, but limited by a global donor heart shortage. Consequently, innovative solutions to increase availability and utilisation are rapidly expanding. Clinically relevant preclinical models essential evaluating interventions human translation, yet few exist that accurately mimic all key HTx components, incorporating injuries beginning in donor, through recipient. To...
The discovery of biological subphenotypes in acute respiratory distress syndrome (ARDS) might offer a new approach to ARDS general and possibly targeted treatment, but little is known about the underlying biology yet. To validate our recently described ovine phenotypes model, we compared subset messenger ribonucleic acid (mRNA) markers leukocytes as reported before display differential expression between human lung tissue model (phenotype 1 (Ph1): hypoinflammatory; phenotype 2 (Ph2):...
Abstract Background Escherichia coli is the most common cause of human bloodstream infections and bacterial sepsis/septic shock. However, translation preclinical septic shock resuscitative therapies remains limited mainly due to low-fidelity available models in mimicking clinical illness. To overcome translational barrier, we sought replicate sepsis complexity by creating an acutely critically-ill model undergoing active 48-h intensive care management. Aim develop a clinically relevant...
Abstract Open-lung ventilation during cardiopulmonary bypass (CPB) in patients undergoing heart transplantation (HTx) is a potential strategy to mitigate postoperative acute respiratory distress syndrome (ARDS). We utilized an ovine HTx model investigate whether open-lung CPB reduces lung damage and complications. Eighteen sheep from were included, with ventilatory interventions randomly assigned CPB: the OPENVENT group received low tidal volume (V T ) of 3 mL/kg positive end-expiratory...
Abstract Background The commonest echocardiographic measurement, left ventricular ejection fraction, can not necessarily predict mortality of recipients following heart transplantation potentially due to afterload dependency. Afterload‐independent stroke work index (LVSWI) is alternatively recommended by the current guideline; however, pulmonary artery catheters are rarely inserted in organ donors most jurisdictions. We propose a novel non‐invasive parameter, Pressure‐Strain Product (PSP),...
Abstract Background Left ventricular stroke work index (LVSWI) and cardiac power (CPI) account for the haemodynamic load of left ventricle are promising prognostic values in cardiogenic shock. However, accurately non‐invasively measuring these parameters during veno‐arterial extracorporeal membrane oxygenation (V‐A ECMO) is challenging potentially biased by circulation. This study aimed to investigate, an ovine model shock, whether Pressure‐Strain Product (PSP), a novel speckle‐tracking...
Despite decades of comprehensive research, Acute Respiratory Distress Syndrome (ARDS) remains a disease with high mortality and morbidity worldwide. The discovery inflammatory subphenotypes in human ARDS provides new approach to study the disease. In two different ovine lung injury models, one induced by additional endotoxin infusion (phenotype 2), mimicking some key features as described hyperinflammatory group, we aim describe protein expression among models. Nine animals on mechanical...