Methionine is a rate-limiting amino-acid for protein synthesis but non-proteinogenic roles on lipid metabolism and oxidative stress have been demonstrated. Contrary to rodents where dietary methionine deficiency led lower adiposity, an increased accretion rate has reported in growing pigs fed deficient diet. This study aimed clarify the effects of different aspects tissue anti-oxidant pathways young pigs. Post-weaned (9.8 kg initial body weight) were restrictively-fed diets providing either...

Abstract Background Poor hygiene of housing induces a systemic inflammatory response. Because inflammation and oxidative stress are processes that can sustain each other, the ways pigs able to activate their antioxidant defenses critical for production performance health during periods when immune system is solicited. Selection also influence reactive oxygen species (ROS) expression levels genes involved in cellular response different tissues. To establish extent by which poor selection feed...

Immortalized bovine mammary epithelial cells (BME-UV1) and immortalized alveolar (MAC-T) have been extensively used as in vitro cell models to understand milk production dairy cows. Precise knowledge about their phenotype performance remains, however, unknown. This study aims characterize MAC-T BME-UV1 profiles when cultured two-dimensional adherent, three-dimensional adherent (Matrigel), no [ultralow attachment (ULA)] supports. were compared according proliferation capacities specific...

Whether neurosteroids regulate the synthesis of long chain polyunsaturated fatty acids in brain cells is unknown. We examined influence 17-beta-estradiol (E2) on capacity SH-SY5Y supplemented with alpha-linolenic acid (ALA), to produce eicosapentaenoic (EPA), docosapentaenoic (DPA) and docosahexaenoic (DHA). Cells were incubated for 24 or 72 h ALA added alone combination E2 (ALA + E2). Fatty analyzed by gas chromatography ethanolamine glycerophospholipids (EtnGpl) phosphatidylcholine...

A better understanding of the control body fat distribution and muscle development is upmost importance for both human animal physiology. This requires a knowledge features physiology adult stem cells in adipose tissue skeletal muscle. Thus objective current study was to determine type proportion these growing pigs. The different cell subsets stromal vascular isolated from tissues were characterized by flow cytometry using surface markers (CD11b, CD14, CD31, CD34, CD45, CD56, CD90). Adipose...

The plasticity of the mammary gland relies on adult stem cells (MaSCs) and their progenitors, which give rise to various populations epithelial (MECs). To face global challenges, an in-depth characterization milk-producing animal is required, select more sustainable robust dairy cows. identification MaSC progenitors will also provide innovative tools in veterinary/human medicine regarding tissue damage (carcinogenesis, bacterial infections). This study aimed determine dynamics cell...

High-yielding dairy cows are prone to oxidative stress due the high metabolic needs of homeostasis and milk production. Oxidative inflammation tightly linked; therefore, anti-inflammatory and/or natural antioxidant compounds may help improve mammary cell health. Baicalin, one major flavonoids in Scutellaria baicalensis, has properties various types, but its effects on bovine epithelial cells (BMECs) have not been investigated.Explants from glands were collected by biopsy at peak lactation...

In pig husbandry, pregnant females are often exposed to stressful conditions, and their outcomes on maternal offspring health have not been well evaluated. The present study aimed at testing whether improving the welfare of gestating sows could be associated with a better during gestation, changes in composition lacteal secretions improvement piglet survival. Two contrasted group-housing systems for were used, that is, French conventional system slatted floor (C, 49 sows) an enriched using...

Flow cytometry has been used as a routine method to count somatic cells in milk, and ascertain udder health milk quality. However, few studies investigate the viability of even fewer at subpopulation level follow up how can resist various stresses that be encountered during technological processes. To address this issue, flow approach was simultaneously identify cell types bovine using cell-specific antibodies measure among identified subpopulations by live/dead kit. Confirmation performed...

Improving the housing of pregnant sows by giving them more space and access to deep straw had positive effects on their welfare, influenced maternal behavior improved survival offspring. The present study aimed at determining whether these were actually due environmental enrichment provision pellets wood can partly mimic bedding during gestation. Three graded levels used, that were, collective conventional pens slatted floor (C, n = 26), same with manipulable materials distribution after...

Synthesis of docosahexaenoic acid (DHA) from its metabolic precursors contributes to membrane incorporation this FA within the central nervous system. Although cultured neural cells are able produce DHA, DHA contents resulting conversion do not match high values those supplementation with preformed DHA. We have examined whether down-regulate newly formed human neuroblastoma cells. SH-SY5Y were incubated gradual doses alpha-linolenic (alpha-LNA), EPA, or docosapentaenoic (DPA), and into...

Sow environment during gestation can generate maternal stress which could alter foetal development. The effects of two group-housing systems for gestating sows on piglet morphological and physiological traits at birth were investigated. During gestation, reared in a conventional system slatted floor (C, 18 sows), demonstrated as being stressful or an enriched larger pens deep straw bedding (E, 19 sows). On day 105, transferred into identical individual farrowing crates floor. Farrowing was...

Intrauterine variations in nutrient allowance can alter body composition and tissue features of the porcine offspring around birth. This study aimed to determine effects fetal weight between littermates maternal dietary regimen during gestation on muscle traits just before Fourteen pregnant gilts were reared under a conventional (control, CTL; n=7) or an experimental (treatment, TRT; gestation. The treatment provided 70% protein digestible energy contents CTL diet first 70 days then, 115% up...

Piglets are immature at birth, which renders them highly sensitive to environmental conditions during the neonatal period. Lean and fat tissues important components for survival later growth capabilities. This chapter focuses on development of skeletal muscle adipose tissue intra-uterine life, suckling post-weaning. Available data show that profile myosin heavy chain isoforms glycogen content good descriptors maturity around birth. Expression levels key genes such as DLK1 IGF2, regulating...

Dietary methionine affects protein metabolism, lean gain and growth performance acts in the control of oxidative stress. When supplied large excess relative to requirements diets for pigs, positive effects on pork quality traits have been recently reported. This study aimed decipher molecular biochemical mechanisms affected by a dietary supply above loin muscle finishing pigs. During last 14 days before slaughter, crossbred female pigs (n = 15 pigs/diet) were fed diet supplemented with...