A5101950225- DNA Repair Mechanisms
- Meat and Animal Product Quality
- Malaria Research and Control
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Animal Nutrition and Physiology
- Mosquito-borne diseases and control
- Chromosomal and Genetic Variations
- Cancer-related Molecular Pathways
- Lipid Membrane Structure and Behavior
- Phytochemicals and Antioxidant Activities
- Carcinogens and Genotoxicity Assessment
- Fermentation and Sensory Analysis
- Thyroid Cancer Diagnosis and Treatment
- Trypanosoma species research and implications
- RNA modifications and cancer
- Food composition and properties
- Cancer Risks and Factors
- Sustainable Agricultural Systems Analysis
- Sperm and Testicular Function
- Plant Genetic and Mutation Studies
- Synthesis and Biological Activity
- DNA and Nucleic Acid Chemistry
- Invertebrate Immune Response Mechanisms
- Multiple and Secondary Primary Cancers
Hospital General de Elda
2019-2024
National University of Santiago del Estero
2013-2023
Vall d'Hebron Institute of Oncology
2021
Ludwig Cancer Research
2009-2016
Centre for Biomedical Network Research on Rare Diseases
2011-2013
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2011-2013
PharmaMar (Spain)
2010-2013
Ludwig Cancer Research
2012
Universidad de Sevilla
1997-2010
University of California, San Diego
2009-2010
The connections between the redox imbalance in post-mortem muscle, early protein oxidation and onset of pale, soft exudative (PSE) condition chicken breast are studied. PSE was induced by incubation carcasses at 37 °C for 200 min. PSE-induced muscle consistently had faster pH decline lower min (5.84 vs. 6.59) 24 h (5.69 5.96), higher L∗ (54.4 57.3), texture water holding capacity (WHC) than normal meat. activities catalase, glutathione peroxidase superoxide dismutase were significantly...
Errors during DNA replication are one likely cause of gross chromosomal rearrangements (GCRs). Here, we analyze the role RNase H2, which functions to process Okazaki fragments, degrade transcription intermediates, and repair misincorporated ribonucleotides, in preventing genome instability. The results demonstrate that rnh203 mutations result a weak mutator phenotype growth defects synergistic increases GCR rates when combined with affecting other metabolism pathways, including homologous...
Abstract Gross chromosomal rearrangements (GCRs) play an important role in human diseases, including cancer. The identity of all Genome Instability Suppressing (GIS) genes is not currently known. Here multiple Saccharomyces cerevisiae GCR assays and query mutations were crossed into arrays mutants to identify progeny with increased rates. One hundred eighty two GIS identified that suppressed formation. Another 438 cooperatively acting genes, but the genome instability caused by individual...
Many of the mutations reported as potentially causing Lynch syndrome are missense in human mismatch repair (MMR) genes. Here, we used a Saccharomyces cerevisiae -based system to study polymorphisms and suspected MMR genes by modeling them at appropriate S. chromosomal locus determining their effect on mutation rates. We identified number weak alleles gene that capable interacting with other produce strong polygenic defects. also MSH2 act if they inactivate Msh2-Msh3 mispair recognition...
In this paper, a laboratory-scale experimentation allowed comparing the performances of two upflow anaerobic packed-bed filters filled with different packing materials and operating at mesophilic conditions (30 degreeC) for treating slaughterhouse wastewaters. Methane production was experimentally evaluated considering volumetric organic loading rates as well feeding overloading conditions. Although filter declined higher than 6 kg CODin m-3 d-1 , chemical oxygen demand (COD) removal...
Unbiased forward genetic screens for mutations causing increased gross chromosomal rearrangement (GCR) rates in Saccharomyces cerevisiae are hampered by the difficulty reliably using qualitative GCR assays to detect mutants with small but significantly rates. We therefore developed a bioinformatic procedure genome-wide functional genomics identify and prioritize candidate GCR-suppressing genes on basis of shared drug sensitivity suppression similar interactions as known suppressors. The...
Nonsyndromic familial non-medullary thyroid cancer (FNMTC) represents 3–9% of cancers, but the susceptibility gene(s) remain unknown. We designed this multicenter study to analyze families with nonsyndromic FNMTC and identify candidate genes. performed exome sequencing DNA from four affected individuals one kindred, five cases FNMTC. Single Nucleotide Variants, insertions deletions that segregated all members, were analyzed by Sanger in 44 additional (37 two seven three or more members), as...
The Asf1 and Rad6 pathways have been implicated in a number of common processes such as suppression gross chromosomal rearrangements (GCRs), DNA repair, modification chromatin, proper checkpoint functions. We examined the relationship between different gene products postreplication repair (PRR) GCRs, function, sensitivity to hydroxyurea (HU) methyl methanesulfonate (MMS), ubiquitination proliferating cell nuclear antigen (PCNA). found that defects PRR pathway Siz1/Srs2 homologous...
High-oxygen packaging atmosphere (High-Ox-MAP) promotes meat protein oxidation and leads to texture deterioration. This study was conceived assess the extent which sprayed fruit extracts could inhibit oxidative damage proteins in lamb cutlets subjected High-Ox-MAP (10 days/4 °C) subsequent roasting min/180 °C). Extracts from oaknut (Quercus ilex subsp. ballota; QI), rose hips (Rosa canina L.; RC), common hawthorn (Crataegus monogyna Jacq.; CM) strawberry tree (Arbutus unedo AU) were...
The aqueous and methanol extracts of Achillea ageratum L. (Asteraceae) have been evaluated for analgesic, antipyretic antiinflammatory properties in mice rats several experimental models. extract exhibited significant activity the analgesic assays but was unable to reduce hyperthermia. Moreover, exerted effects three pharmacological actions. phytochemical investigation this latter revealed presence flavonic compounds: luteolin, quercetin 7-O-β-luteolin. © 1997 John Wiley & Sons, Ltd.
Quality and safety are important challenges in traditional fermented sausage technology. Consequently, the development of a tailored starter culture based on indigenous microbiota constitutes an interesting alternative. In present study, spontaneously goat meat sausages were created analyzed using physicochemical microbiological approach. Thereafter 170 lactic acid bacteria (LAB) strains isolated preliminary characterized by phenotypic assays. The hygienic technological properties, growth...
Background and Purpose We have previously shown that cells with a defective Fanconi anaemia ( FA ) pathway are hypersensitive to trabectedin, DNA ‐binding anti‐cancer tetrahydroisoquinoline DBAT whose adducts functionally mimic inter‐strand cross link ICL ). Here we expand these observations new s investigate whether our findings in primary untransformed can be reproduced human cancer cells. Experimental Approach Initially, the sensitivity of transformed cells, deficient or not one component...
The objective of this research was to evaluate the effectiveness antioxidant-rich extracts from rose hip (Rosa canina L.; RC) and hawthorn (Crataegus monogyna Jacq.; CM) at minimizing oxidative damage proteins lipids in beef patties subjected a high‑oxygen (HiOx-MAP) vacuum (Vacuum) packaging atmosphere. RC CM were characterized by quantifying bioactive compounds, namely, phenolic tocopherols vitamin C. Both fruits had high concentrations with having highest total C content. Yet, most...
It has long been observed that there are families in which non-medullary thyroid cancer (NMTC) occurs, but few syndromes and genes have described to date. Proteins the shelterin complex implied cancer. Here, we studied affected by NMTC (FNMTC).
Se evaluaron parámetros de desempeño productivo y calidad la res carne 18 novillos raza Criollo Argentino (CrA), Braford (Bra) cruza F1 CrA x Bra (F1). Los animales fueron sometidos a un engorde corral durante 70 días sacrificados una edad promedio 19,3 meses. lograron mayor engrasamiento subcutáneo que (8.05 vs 5.30 mm, P = 0.0008), aunque no se hallaron diferencias 1 en el peso vivo definitivo (418 kg, 0.819), ni ganancia diaria (1.39 kg d , 0.8196) sobre nivel grasa intramuscular (1.78...
En los últimos años, las series de tiempo han tenido desarrollos y aplicaciones importantes en el área la salud; por ello, presente artículo muestra una metodología ensayada estudios anteriores, que se esbozan pasos análisis para un enfoque clásico. El objetivo permite identificar buen modelo pronóstico usando técnicas suavización: exponencial, simple, Holt Winter. Además, realiza validación supuestos residuales, dando confiabilidad a resultados del encontrado. Para aplicar procedimiento...
High prevalence of placental-derived complications, such as preeclampsia and intrauterine growth restriction, has been reported in women with breast cancer (BC) treated chemotherapy during pregnancy (PBC-CHT). Aim: To ascertain whether PBC-CHT is associated an imbalance angiogenic factors, surrogate markers for placental insufficiency, that could explain perinatal outcomes. Methods: Prospective study between 2012 2016 a single institution. Soluble fms-like tyrosine kinase (sFlt-1), factor...