A5058542325- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Tissue Engineering and Regenerative Medicine
- Genetics, Aging, and Longevity in Model Organisms
- Physiological and biochemical adaptations
- Additive Manufacturing and 3D Printing Technologies
- Anatomy and Medical Technology
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- 3D Printing in Biomedical Research
- Spaceflight effects on biology
- Amphibian and Reptile Biology
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
University of Colorado Boulder
2024-2025
University of Colorado System
2025
Fred Hutch Cancer Center
2020-2021
Cancer Research Center
2021
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2021
A longstanding hypothesis is that chromatin fiber folding mediated by interactions between nearby nucleosomes represses transcription. However, it has been difficult to determine the relationship local compaction and transcription in cells. Further, global changes diameters have not observed, even interphase mitotic chromosomes. We show an increase range of inter-nucleosomal contacts quiescent yeast drives fibers genome-wide. Unlike actively dividing cells, cells require a basic patch...
Abstract The study of nontraditional model organisms, particularly those exhibiting extreme phenotypes, offers unique insights into adaptive mechanisms stress response and survival. Snakes, with their remarkable physiological, metabolic, morphological adaptations, serve as powerful models for investigating these processes. Pythons are a organism that have been studied metabolic physiological plasticity. To date, the Burmese python ( Python bivittatus ) is only member Pythonidae family to...
SUMMARY The heart is a highly plastic organ that responds to diverse stimuli modify form and function. molecular mechanisms of adaptive physiological cardiac hypertrophy are well-established; however, the regulation regression poorly understood. To identify features regression, we studied Burmese pythons which experience reversible following large, infrequent meals. Using multi-omics screens followed by targeted analyses, found forkhead box protein O1 (FoxO1) transcription factor signaling,...
As ambush-hunting predators that consume large prey after long intervals of fasting, Burmese pythons evolved with unique adaptations for modulating organ structure and function. Among these is cardiac hypertrophy develops within three days following a meal (Andersen et al., 2005, Secor, 2008), which we previously showed was initiated by circulating growth factors (Riquelme 2011). Postprandial in also rapidly regresses subsequent fasting (Secor, 2008); however, the molecular mechanisms...
ABSTRACT 3D in vitro engineered heart tissue (EHT) models recapitulate aspects of native cardiac physiology but are often limited by scalability, cost, and reproducibility. Here, we report a simple, one-step method for rapid (∼minutes) fabrication molds using digital light processing (DLP)-based printing that support the formation EHTs human induced pluripotent stem cell derived cardiomyocytes (iPSC-CMs) with high reproducibility (>95% efficiency) varied designs (e.g., length, aspect...
Abstract Determining the conformation of chromatin in cells at nucleosome level and its relationship to cellular processes has been a central challenge biology. We show that quiescent yeast, widespread transcriptional repression coincides with local compaction fibers into structures are less condensed more heteromorphic than canonical 30-nanometer forms. Acetylation or substitution H4 tail residues decompacts leads global de-repression. Fiber decompaction also increases rate loop extrusion...
Therapies for end-stage heart failure are lacking and the number of patients awaiting transplants far outweighs availability. Left ventricular assist devices (LVADs) take over cardiac pump function used as a bridge-to-transplant. Notably, some on LVADs experience recovery reversal pathological remodeling; however, molecular mechanisms underlying these favorable outcomes unknown. In this study, we studied 19 paired pre- post-LVAD samples with 10 clinically categorized responders 9...
Properly coordinated folding and assembly of myosin into the sarcomere thick filament is critical for cardiac skeletal muscle development, function, growth, maintenance. The motor domain heavy chain (MHC) requires muscle-specific chaperones proper folding, making it currently impossible to fold in non-muscle cells. Previous studies found that HSP90 UNC45B are necessary, but not sufficient, domain, indicating other factors involved. Defining minimal set would allow development an improved...
While cell models have been used to study cardiac diseases, there is currently no standardization or fully mature in vitro system. Obtaining a cardiomyocyte (CM) phenotype necessary create physiologically relevant environment and accurately model diseases. Increased success maturation of human induced pluripotent stem derived CMs (hiPS-CMs) has achieved 3D the form engineered heart tissues (EHTs). Digital light processing (DLP) offers high throughput efficient method fabricate hydrogels that...