A5059097854- Mechanical Circulatory Support Devices
- Cardiac Structural Anomalies and Repair
- Heart Failure Treatment and Management
- Transplantation: Methods and Outcomes
- Cardiac pacing and defibrillation studies
- Cardiovascular Function and Risk Factors
- Cardiac Arrest and Resuscitation
- Renal Transplantation Outcomes and Treatments
- Cardiac Arrhythmias and Treatments
- Cardiomyopathy and Myosin Studies
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cardiac electrophysiology and arrhythmias
- Viral Infections and Immunology Research
- Organ Transplantation Techniques and Outcomes
- Medication Adherence and Compliance
- Cardiac Valve Diseases and Treatments
- Cardiovascular Effects of Exercise
- Atrial Fibrillation Management and Outcomes
- Neuroscience and Neuropharmacology Research
- Congenital Heart Disease Studies
- Health Systems, Economic Evaluations, Quality of Life
- Liver Disease and Transplantation
- Peptidase Inhibition and Analysis
- Eosinophilic Disorders and Syndromes
- Aortic Disease and Treatment Approaches
University of Colorado Denver
2016-2025
University of Colorado Anschutz Medical Campus
2016-2025
University of Colorado Colorado Springs
2024
International Society for Heart and Lung Transplantation
2024
IFC Research (United Kingdom)
2024
University of Colorado System
2008-2023
The Medical Center of Aurora
2010-2022
University of Colorado Boulder
2014-2021
Rosenthaler + Partner (Switzerland)
2020
University of New Mexico
2019
Current heart failure (HF) treatment is based on targeting symptoms and left ventricle dysfunction severity, relying a common HF pathway paradigm to justify treatments for patients. This strategy may belie an incomplete understanding of heterogeneous underlying mechanisms could be barrier more precise treatments. We hypothesized we use RNA-sequencing (RNA-seq) in human tissue delineate etiology-specific gene expression signatures.RNA-seq from 64 ventricular samples: 37 dilated (DCM), 13...
The purpose of this study was to assess the phenotype Filamin C (FLNC) truncating variants in dilated cardiomyopathy (DCM) and understand mechanism leading an arrhythmogenic phenotype. Mutations FLNC are known lead skeletal myopathies, which may have associated cardiac component. Recently, clinical spectrum mutations has been recognized include a cardiac-restricted presentation absence muscle involvement. A population 319 U.S. European DCM families evaluated using whole-exome targeted...
The heart grows in response to pathological and physiological stimuli. former often precedes cardiomyocyte loss failure; the latter paradoxically protects enhances cardiomyogenesis. mechanisms underlying these differences remain incompletely understood. Although long noncoding RNAs (lncRNAs) are important cardiac development disease, less is known about their roles hypertrophy or cardiomyogenesis.RNA sequencing was applied hearts from mice after 8 weeks of voluntary exercise-induced...
The histone deacetylase inhibitor ITF2357 (givinostat) prevents diastolic dysfunction by enhancing cardiac myofibril relaxation.
Negative alterations of mitochondria are known to occur in heart failure (HF). This study investigated the novel mitochondrial-targeted therapeutic agent elamipretide on mitochondrial and supercomplex function failing human hearts ex vivo. Freshly explanted nonfailing ventricular tissue from children adults was treated with elamipretide. Mitochondrial oxygen flux, complex (C) I CIV activities, in-gel activity assembly were measured. impaired heart, CI supercomplex-associated significantly...
Background: In the phase 3 randomized controlled study, ATTRibute-CM, acoramidis, a transthyretin (TTR) stabilizer, demonstrated significant efficacy on primary endpoint. Participants with amyloid cardiomyopathy (ATTR-CM) who completed ATTRibute-CM were invited to enroll in an open-label extension study (OLE). We report and safety data of acoramidis participants enrolled ongoing OLE. Methods: previously received through Month 30 (M30) continued receive it (continuous acoramidis), those...
Pulsatility seems to have a teleological role because evolutionary hierarchy favors higher ordered animals with more complex, multichamber circulatory systems that generate pulse pressure compared lower animals. Yet despite years of such natural selection, the modern generation continuous-flow left ventricular assist devices (CF-LVADs) been increasingly used for last decade created unique physiology characterized by nonpulsatile, nonlaminar blood flow profile absence usual large elastic...
BRD4 governs pathological cardiac gene expression by binding acetylated chromatin, resulting in enhanced RNA polymerase II (Pol II) phosphorylation and transcription elongation. Here, we describe a signal-dependent mechanism for the regulation of cardiomyocytes. is suppressed microRNA-9 (miR-9), which targets 3′ UTR Brd4 transcript. In response to stress stimuli, miR-9 downregulated, leading derepression enrichment at long-range super-enhancers (SEs) associated with genes. A mimic represses...
Background— The effects of nonpulsatile flow on the aorta are unknown. Our aim was to examine structure from patients with continuous-flow left ventricular assist devices (LVADs) and directly measure aortic wall composition stiffness. Methods Results— Age-matched samples were collected consecutive heart failure (HF) at time transplantation compared nonfailing donor hearts. An unbiased stereological approach used quantify morphometry composition, biomechanical testing performed determine...
A 59-year-old woman with a history of bilateral total hip replacements and knee replacement sees her physician for cough, exertional dyspnea, foot swelling that had developed 2 weeks earlier while she was on vacation in Europe.
<h3>Importance</h3> The prevalence of nonischemic dilated cardiomyopathy (DCM) is greater in individuals African ancestry than European ancestry. However, little known about whether the difference or outcomes associated with functional genetic variants. <h3>Objective</h3> We hypothesized that Bcl2-associated anthanogene 3 (<i>BAG3</i>) variants were DCM. <h3>Design</h3> This multicohort study the<i>BAG3</i>genotype patients uses DNA obtained from American enrolled clinical studies: Genetic...
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are a powerful platform for biomedical research. However, they immature, which is barrier to modeling adult-onset cardiovascular disease. Here, we sought develop simple method that could drive cultured hiPSC-CMs toward maturity across number of phenotypes, with the aim utilizing mature model human were in fatty acid-based medium and plated on micropatterned surfaces. These cells display many characteristics adult...
Passive stiffness of the heart is determined largely by extracellular matrix and titin, which functions as a molecular spring within sarcomeres. Titin stiffening associated with development diastolic dysfunction (DD), while augmented titin compliance appears to impair systolic performance in dilated cardiomyopathy. We found that myofibril was elevated mice lacking histone deacetylase 6 (HDAC6). Cultured adult murine ventricular myocytes treated selective HDAC6 inhibitor also exhibited...
<h3>Importance</h3> Orthotopic heart transplant (OHT) recipients are at increased risk for morbidity and mortality after SARS-CoV-2 infection. Although antibody response to COVID-19 vaccination is lower in solid organ recipients, there has been no study assessing the safety effectiveness of OHT recipients. <h3>Objective</h3> To assess associations with infection clinical outcomes a large population adult <h3>Design, Setting, Participants</h3> This case-control examined data from US program...
Unloading a failing heart with left ventricular assist device (LVAD) can improve ejection fraction (EF) and LV size; however, recovery LVAD explantation is rare. We hypothesized that evaluation of myocyte contractility biochemistry at the sarcomere level before after may explain organ-level changes.Paired tissue samples were frozen from 8 patients nonischemic cardiomyopathy implantation (before LVAD) cardiac transplantation (after LVAD). These compared nonfailing hearts. Isolated skinned...
Purpose To develop, teach, and supervise a structured process for handing off patient care to evaluate its effect on interns' knowledge, skills, attitudes toward handoffs. Method The authors developed formal interns the medicine ward services hand at their teaching hospital. In July 2006, attending physicians began teach process. entire handoff program (the process, teaching, supervision), were surveyed first day during last week of each month long rotations. Results From June through...