A5070098548- Wnt/β-catenin signaling in development and cancer
- Proteoglycans and glycosaminoglycans research
- Hedgehog Signaling Pathway Studies
- Protein Hydrolysis and Bioactive Peptides
- Glycosylation and Glycoproteins Research
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Cancer-related gene regulation
- Parathyroid Disorders and Treatments
- RNA modifications and cancer
- Spectroscopy and Chemometric Analyses
- Enzyme Structure and Function
- Fibroblast Growth Factor Research
- Genetic Syndromes and Imprinting
- Cancer-related molecular mechanisms research
- HER2/EGFR in Cancer Research
- Photoreceptor and optogenetics research
- Biochemical effects in animals
- Spectroscopy Techniques in Biomedical and Chemical Research
- Advanced Chemical Sensor Technologies
- Cholesterol and Lipid Metabolism
- Kruppel-like factors research
- Dialysis and Renal Disease Management
- Optical Imaging and Spectroscopy Techniques
- Hippo pathway signaling and YAP/TAZ
Peking University
2015-2025
Zhongda Hospital Southeast University
2021-2025
Hangzhou Hospital of Traditional Chinese Medicine
2025
Zhejiang Chinese Medical University
2025
First Affiliated Hospital of Xi'an Jiaotong University
2021-2025
Punan Hospital
2024
Central South University
2022-2023
Sunnybrook Health Science Centre
2009-2023
University of Toronto
2009-2023
Xiangya Hospital Central South University
2022-2023
Glypicans are a family of heparan sulfate proteoglycans that linked to the cell surface through glycosyl–phosphatidylinositol anchor. One member this family, glypican-3 (Gpc3), is mutated in patients with Simpson-Golabi-Behmel syndrome (SGBS). These display pre- and postnatal overgrowth, varying range dysmorphisms. The clinical features SGBS very similar more extensively studied Beckwith-Wiedemann (BWS). Since BWS has been associated biallelic expression insulin-like growth factor II...
Loss-of-function mutations of the GPC3 gene are cause human Simpson-Golabi-Behmel syndrome. Based on overgrowth phenotype syndrome patients and key role played by insulin-like growth factor (IGF) signaling system in regulating embryonic growth, it was speculated that regulates IGF signaling. In order to test validity this hypothesis, we mated knockout mice with insulin receptor substrate-1 (IRS-1) nullizygous mice. We found organism independent IRS-1, suggesting does not modulate Instead,...
Glypicans are heparan sulfate proteoglycans that attached to the cell surface by a GPI (glycosylphosphatidylinositol) anchor. regulate activity of Wnts, Hedgehogs, bone morphogenetic proteins and fibroblast growth factors. In particular case it has been proposed GPI-anchored glypicans stimulate Wnt signalling facilitating and/or stabilizing interaction between Wnts their receptors. On other hand, when secreted extracellular environment, they can act as competitive inhibitors Wnt. Genetic...
Glypican-3 (GPC3) is a proteoglycan that bound to the cell surface. It expressed by most hepatocellular carcinomas (HCCs), but not normal hepatocytes. GPC3 stimulates HCC growth promoting canonical Wnt signaling. Because glypicans interact with Wnts, it has been proposed these proteoglycans stimulate signaling increasing amount of at membrane, facilitating in this way interaction factor its receptor Frizzled. However, study we demonstrate plays more direct role stimulation Specifically, show...
ABSTRACT Single cell clones were isolated from the human teratoma line, Tera-2. The cells of three these studied. progressively growing shown to be tumorigenic, and they characterized by lack expression β 2β2-microglobulinand HLA-A,B,C determinants on surface. majority expressed Thy-1 antigen a 90 × 103 molecular weight protein recognized monoclonal antibody F10.44.2; between third half sugar specificities detected anti-SSEA-1 antibody. In response 5 10−5 M-retinoic acid applied in monolayer...
Glypican-5 (GPC5) is one of the six members glypican family. It has been previously reported that GPC5 stimulates proliferation rhabdomyosarcoma cells. In this study, we show stimulatory activity a result its ability to promote Hedgehog (Hh) signaling. We have shown GPC3, another member family, inhibits Hh signaling by competing with Patched 1 (Ptc1) for binding. Furthermore, showed GPC3 binds through core protein but not Ptc1. paper, demonstrate increases binding Sonic also both and Ptc1...
Rheumatoid arthritis (RA), a common autoimmune disease, is extremely damaging to human health. Fibroblast-like synoviocytes (FLSs) have vital role in the occurrence and development of RA. Methyltransferase-like 3 (METTL3), which crucial component N 6 -methyladenosine (m A) methyltransferase complex, involved progression many diseases. In this study, we explored METTL3 inflammatory response proliferation, invasion, migration FLSs. We used RA synovial tissues adjuvant-induced (AIA) animal...
OCI-5/GPC3 is a member of the glypican family. Glypicans are heparan sulfate proteoglycans that bound to cell surface through glycosyl-phosphatidylinositol anchor. It has recently been shown gene mutated in patients with Simpson-Golabi-Behmel Syndrome (SGBS), an X-linked disorder characterized by pre- and postnatal overgrowth various visceral skeletal dysmorphisms. Some these dysmorphisms could be result deficient growth inhibition or apoptosis certain types during development. Here we...
Transforming growth factor-α (TGF-α) is a member of the EGF factor family. Both transmembrane TGF-α and proteolytically released soluble can bind to EGF/TGF-α tyrosine kinase receptor (EGFR) activate EGFR-induced signaling pathways. We now demonstrate that physically interacts with CD9, protein four membrane spanning domains frequently coexpressed in carcinomas. This interaction was mediated through extracellular domain TGF-α. CD9 expression strongly decreased factor– PMA- induced...
OCI-5 encodes the rat homologue of glypican-3, a membrane-bound heparan sulfate proteoglycan that is mutated in Simpson-Golabi-Behmel overgrowth syndrome. and glypican-3 are 95% identical. It has been recently suggested interacts with insulin-like growth factor-2 (IGF-2) this interaction regulates IGF-2 activity. We report here we have transfected into two different cell lines, not able to detect an between produced by cells IGF-2. On other hand, found FGF-2, as already shown for glypican-1....
Abstract The heterogeneity of the molecular pathology HCC poses a formidable obstacle to development non‐cytotoxic therapies. Several pro‐tumorigenic signaling pathways can be aberrantly activated in HCC, including those triggered by Wnts. Glypican‐3 (GPC3), membrane‐bound heparan sulfate proteoglycan that is overexpressed most HCCs, promotes growth these tumors stimulating Wnt signaling. Because GPC3 binds with high affinity Wnts, and its growth‐promoting activity requires attachment cell...
The effects of desalted duck egg white peptides (DPs) on calcium absorption were investigated in three models: Caco-2 cell monolayer model, population and everted intestinal sac model. DPs found to enhance transport may do so by acting as carriers interacting with the membrane open a special Ca(2+) channel, whereas paracellular pathway make only minor contribution. Structure characterization demonstrated important roles seven crucial peptides, such VSEE LYAEE, binding promoting uptake. Three...
To elucidate the genetic effect involved in premalignant progression of chronic inflammation to cancer, we performed microRNA and mRNA profiling oral lichen planus (OLP), squamous cell carcinoma (OSCC), normal tissue from same patients. We demonstrate involvement a suppressive microRNA, miR-375, regulation this via KLF5, transcription factor that modulates expression genes contributing proliferation apoptosis. found miR-375 abundance decreased tissues with state OLP subsequently OSCC....
Abstract BACKGROUND In order to utilize the industrial by‐product ‘salted duck egg white’ as novel iron additives, effects of desalted white peptides‐ferrous chelate (DPs‐Fe) on promotion uptake and structure were investigated. RESULTS Different doses DPs‐Fe given sulfate (FeSO 4 ) was used a positive control. After three weeks, hemoglobin (Hb), hematocrit (HCT), red blood cells (RBCs), mean corpuscular volume (MCV), serum (SI) ferritin (SF) in iron‐deficiency anemia (IDA) rats could be...
Background: Oxidative stress contributes to adverse atrial remodeling in diabetes mellitus. This can be prevented by the PPAR-γ agonist pioglitazone via its antioxidant and anti-inflammatory effects. In this study, we examined molecular mechanisms underlying protective effects of on a rabbit model diabetes. Methods: Rabbits were randomly divided into control, diabetic, pioglitazone-treated diabetic groups. Echocardiographic, hemodynamic, electrophysiological parameters measured. Serum...
Autosomal-recessive omodysplasia (OMOD1) is a genetic condition characterized by short stature, shortened limbs, and facial dysmorphism. OMOD1 caused loss-of-function mutations of glypican 6 (GPC6). In this study, we show that GPC6-null embryos display most the abnormalities found in patients Hedgehog (Hh) signaling significantly reduced long bones these embryos. The Hh-stimulatory activity GPC6 was also observed cultured cells, where GPC increased binding Hh to Patched 1 (Ptc1). Consistent...
Background Vascular calcification is highly prevalent and associated with mortality in hemodialysis patients. However, extreme splanchnic arterial calciphylaxis poor prognosis raises questions regarding the reliability of previous vascular scoring methods. Therefore, this study aimed to examine distribution characteristics abdominal aortic branch identify a more reliable predictor
Background In diabetic patients, non-diabetic kidney disease (NDKD) may occur independently or alongside (DKD). This study explored the utility of biopsy in type 2 diabetes mellitus (T2DM) patients and predictability diagnosing DKD combined with NDKD using clinical laboratory data. Methods retrospective examines medical records T2DM who underwent percutaneous renal at Hangzhou TCM Hospital, Zhejiang Chinese Medical University, from 2012 to 2023. The patient’s demographic, clinical, blood...