A5013233999- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Seaweed-derived Bioactive Compounds
- Legume Nitrogen Fixing Symbiosis
- Polysaccharides and Plant Cell Walls
- Plant Pathogenic Bacteria Studies
- Polysaccharides Composition and Applications
- Polyamine Metabolism and Applications
- Microbial Community Ecology and Physiology
- Fibroblast Growth Factor Research
- Hedgehog Signaling Pathway Studies
- Algal biology and biofuel production
- Blood properties and coagulation
- Estrogen and related hormone effects
- Sulfur Compounds in Biology
- Protein Hydrolysis and Bioactive Peptides
- Electrochemical sensors and biosensors
- Epigenetics and DNA Methylation
- Analytical Chemistry and Sensors
- Computational Drug Discovery Methods
- Synthesis and biological activity
- Amino Acid Enzymes and Metabolism
- HIV Research and Treatment
Shandong University
2016-2025
State Key Laboratory of Microbial Technology
2015-2025
Ocean University of China
2004-2025
China Aerospace Science and Industry Corporation (China)
2012-2022
Shandong Agricultural University
2021
Beijing Institute of Technology
2021
Institute of Hydrobiology
2018
National University of Rosario
2018
Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences
2018
Sunnybrook Research Institute
2009-2011
Alginate lyases can fully degrade alginate into various size-defined unsaturated oligosaccharide products by β -elimination. Here, we identified the bifunctional endolytic lyase Aly35 from marine bacterium Vibrio sp. Strain H204. The enzyme is classified polysaccharide 7 superfamily and contains two catalytic domains. relationship function of domains are not well known. Thus, full-length recombinant its truncated proteins Aly35-CD1 (catalytic domain 1), Aly35-CD2 2 domain) were constructed....
Glypican-5 (GPC5) is one of the six members glypican family. It has been previously reported that GPC5 stimulates proliferation rhabdomyosarcoma cells. In this study, we show stimulatory activity a result its ability to promote Hedgehog (Hh) signaling. We have shown GPC3, another member family, inhibits Hh signaling by competing with Patched 1 (Ptc1) for binding. Furthermore, showed GPC3 binds through core protein but not Ptc1. paper, demonstrate increases binding Sonic also both and Ptc1...
ABSTRACT Alginate lyases are important tools for oligosaccharide preparation, medical treatment, and energy bioconversion. Numerous alginate have been elucidated. However, relatively little is known about their substrate degradation patterns product-yielding properties, which a limit to wider enzymatic applications further enzyme improvements. Herein, we report the characterization module truncation of Aly5, first lyase obtained from polysaccharide-degrading bacterium Flammeovirga . Aly5...
We report herein imaging cell surface glycosylation by using click chemistry and DNA rolling circle amplification (RCA) to improve detection sensitivity..
Dimethylsulfoniopropionate (DMSP) is an abundant and ubiquitous organosulfur molecule in marine environments with important roles global sulfur nutrient cycling. Diverse DMSP lyases some algae, bacteria, fungi cleave to yield gaseous dimethyl sulfide (DMS), infochemical atmospheric chemistry. Here, we identified a novel ATP-dependent lyase, DddX. DddX belongs the acyl-CoA synthetase superfamily distinct from eight other known lyases. catalyses conversion of DMS via two-step reaction:...
Lyases cleave glycosaminoglycans (GAGs) in an eliminative mechanism and are important tools for the structural analysis oligosaccharide preparation of GAGs. Various GAG lyases have been identified from terrestrial but not marine organisms even though animals rich GAGs with unique structures functions. Herein we isolated a novel lyase first time bacterium Vibrio sp. FC509 then recombinantly expressed characterized it. It showed strong activity toward hyaluronan (HA) chondroitin sulfate (CS)...
Endo-type alginate lyases usually degrade completely into various sizedefined unsaturated oligosaccharide products (≥disaccharides), while exoenzymes primarily produce monosaccharide including saturated mannuronate (M) and guluronate (G) units particularly units.Recently, two bifunctional have been identified as endolytic but M-and G-producing with variable action modes.However, -producing remain undiscovered.Herein, a new Flammeovirga protein, Aly2, was classified the polysaccharide lyase 7...
Accumulating evidence suggests the involvement of chondroitin sulfate (CS) and dermatan (DS) hybrid chains in brain's development critical roles for oversulfated disaccharides IdoUA residues growth factor-binding neuritogenic activities these chains. In pursuit sources CS/DS with unique structures, activity, therapeutic potential, two novel preparations were isolated from shark liver by anion exchange chromatography. The major (80%) low sulfated minor (20%) highly fractions had an average...
Abstract Background Macroalgae and microalgae, as feedstocks for third-generation biofuel, possess competitive strengths in terms of cost, technology economics. The most important compound brown macroalgae is alginate, the synergistic effect endolytic exolytic alginate lyases plays a crucial role saccharification process transforming into biofuel. However, there are few studies on lyases, especially those from same bacterial strain. Results In this study, lyase AlyPB1 AlyPB2 were identified...
Bifunctional alginate lyases can efficiently degrade comprised of mannuronate (M) and guluronate (G), but their substrate-degrading modes have not been thoroughly elucidated to date. In this study, we present Aly1 as a novel bifunctional endolytic lyase the genus Flammeovirga The recombinant enzyme showed optimal activity at 50°C pH 6.0. produced unsaturated disaccharide (UDP2) trisaccharide fractions final main digests. Primary substrate preference tests further structure identification...
Abstract The collagenases of Vibrio species, many which are pathogens, have been regarded as an important virulence factor. However, there is little information on the structure and collagenolytic mechanism collagenase. Here, we report crystal collagenase module (CM) VhaC conformation in solution. Structural biochemical analyses molecular dynamics studies reveal that triple-helical collagen initially recognized by activator domain, followed subsequent cleavage peptidase domain along with...
Recently, a new class of glycosaminoglycan (GAG) lyases (GAGases) belonging to PL35 family has been discovered with an ultrabroad substrate spectrum that can degrade three types uronic acid-containing GAGs (hyaluronic acid, chondroitin sulfate and heparan sulfate) or even alginate. In this study, the structures GAGase II from Spirosoma fluviale VII Bacteroides intestinalis DSM 17393 were determined at 1.9 2.4 Å resolution, respectively, their catalytic mechanism was investigated by...
Sulfated polymannuronate (SPMG), a novel anti-AIDS drug candidate, combats HIV-1 infection mainly by binding to gp120 protein with high affinity. To explore the structural basis of this anti-HIV-1 action, size-defined oligosaccharides were prepared semi-synthesis or separated from native SPMG. In study, series homogeneously sized SPMG fragments are evaluated for their capacity bind rgp120 using surface plasmon resonance (SPR) analysis. The minimum fragment size that interacts is...
Polygalacturonase-inhibiting proteins (PGIPs) have been shown to recognize fungal polygalacturonases (PGs), which initiate innate immunity in various plant species. Notably, the connection between rice OsPGIPs and PGs Xanthomonas oryzae pv. oryzicola (Xoc), causes bacterial leaf streak (BLS), remains unclear. Here, we show that OsPGIP1 was strongly induced after inoculating with Xoc strain RS105. Furthermore, OsPGIP1-overexpressing (OV) RNA interference (RNAi) lines increased decreased,...
A glassy carbon electrode was modified with graphene. In phosphate buffer solution, the showed an excellent electrocatalytical effect on oxidation of norepinephrine (NE). This further used to determine NE in presence epinephrine, uric acid and ascorbic by cyclic voltammetry. The reduction peak current a linear relationship concentrations range 6.00×10-7 1.20×10-4 mol·L-1, detection limit 4.00×10-7 mol·L-1. can be selectively measuring PBS pH 7.0. method has high sensitivity, selectivity,...