A5059049734- Antifungal resistance and susceptibility
- Fungal Plant Pathogen Control
- Fungal Infections and Studies
- Fungal and yeast genetics research
- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Pneumocystis jirovecii pneumonia detection and treatment
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Slime Mold and Myxomycetes Research
- Microbial Metabolism and Applications
- Biocrusts and Microbial Ecology
- Enzyme-mediated dye degradation
- Mycotoxins in Agriculture and Food
- Insect Resistance and Genetics
- Essential Oils and Antimicrobial Activity
- Microbial Natural Products and Biosynthesis
- Advanced Database Systems and Queries
- Pesticide and Herbicide Environmental Studies
- Ocular Infections and Treatments
- Agriculture, Plant Science, Crop Management
- Plant and Biological Electrophysiology Studies
- Insect and Pesticide Research
- Traditional and Medicinal Uses of Annonaceae
Université Paris Cité
2012-2024
Centre National de la Recherche Scientifique
2020-2024
Sorbonne Paris Cité
2012-2024
Laboratoire Interdisciplinaire des Énergies de Demain
2013-2023
Université Paris-Sud
2012-2014
Délégation Paris 5
2007-2012
Laboratoire de Recherche en Sciences Végétales
2003-2006
Cyclopharma (France)
1998-2002
Sorbonne Université
1999
We describe the acquisition of flucytosine, azole, and caspofungin resistance in sequential Candida glabrata bloodstream isolates collected from a bone marrow transplant patient with clinical failure. Point mutations C. FUR1 (CgFUR1) CgFKS2 overexpression CgCDR1 CgCDR2 were observed resistant isolates.
Inactivation of the FCY2 (cytosine permease), FCY1 deaminase), and FUR1 (uracil phosphoribosyltransferase) genes in Candida lusitaniae produced two patterns resistance to flucytosine. Mutant fur1 demonstrated 5-fluorouracil, whereas mutants fcy1 fcy2 fluconazole presence subinhibitory flucytosine concentrations.
Multi-drug resistant bacteria hazards to the health of humans could be an agent in destruction human generation. Natural products Bacillus species are main source access progressive antibiotics that can a good candidate for discovery novel antibiotics. Wild honey as valuable food has been used medicine with antimicrobial effects.
The aim of this work was to elucidate the molecular mechanisms flucytosine (5FC) resistance and 5FC/fluconazole (FLC) cross-resistance in 11 genetically epidemiologically unrelated clinical isolates Candida lusitaniae. We first showed that levels transcription FCY2 gene encoding purine-cytosine permease (PCP) were similar wild-type strain, 6936. Nucleotide sequencing alleles revealed 5FC 5FC/FLC could be correlated with a cytosine-to-thymine substitution at nucleotide 505 fcy2 genes seven...
Summary Transformation of plant biomass into biofuels may supply environmentally friendly alternative biological sources energy. Laccases are supposed to be involved in the lysis lignin, a prerequisite step for efficient breakdown cellulose fermentable sugars. The role development and degradation nine canonical laccases belonging three different subfamilies one related multicopper oxidase Ascomycota fungus P odospora anserina was investigated by targeted gene deletion. 10 genes were...
In a previous work, we described the possible relationship between defect of purine-cytosine permease and acquisition cross-resistance to antifungal combination flucytosine (5FC) fluconazole (FLC) in Candida lusitaniae (T. Noël, F. François, P. Paumard, C. Chastin, D. Brethes, J. Villard, Antimicrob. Agents Chemother. 47:1275-1284, 2003). Using degenerate PCR chromosome walking, cloned two FCY2-like genes lusitaniae. Northern blot analysis revealed that only one gene was expressed; it named...
Abstract The success of filamentous fungi in colonizing most natural environments can be largely attributed to their ability form an expanding interconnected network, the mycelium, or thallus, constituted by a collection hyphal apexes motion producing hyphae and subject branching fusion. In this work, we characterize network expansion structure fungus Podospora anserina under controlled culture conditions. To end, temporal series pictures dynamics are produced, starting from germinating...
The ascomycete Podospora anserina is a heterothallic filamentous fungus found mainly on herbivore dung. It commonly used in laboratories as model system, and its complete life cycle lasting eight days well mastered vitro. main objective of our team to understand better the global process fruiting body development, named perithecia, induced normally this species by fertilization. Three allelic mutants, pfd3, pfd9, pfd23 (for “promoting development”) obtained UV mutagenesis, were selected view...
In their natural environment, fungi are subjected to a wide variety of environmental stresses which they must cope with by constantly adapting the architecture growing network. this work, our objective was finely characterize thallus development filamentous fungus Podospora anserina different constraints that simple implement in vitro and can be considered as relevant stresses, such nutrient-poor environment or non-optimal temperatures. At Petri dish scale, observations showed fungal is...
Abstract In yeast, external signals such as high osmolarity or oxidant conditions activate the glycerol (HOG) mitogen‐activated protein kinase (MAPK) cascade pathway, which consists of two upstream branches, i.e. Sho1p and Sln1p common downstream elements, including Pbs2p MAPK Hog1p MAPK. We recently showed that Candida lusitaniae SLN1 gene, potentially encoding a histidine receptor, is crucial for oxidative stress adaptation when fungus grows budding yeast during early steps pseudohyphal...
Filamentous fungi are known as prolific untapped reservoirs of diverse secondary metabolites, where genes required for their synthesis organized in clusters. The bioactive properties these compounds closely related to functions fungal biology, which not well understood. In this study, we focused on the Podospora anserina gene cluster responsible biosynthesis sterigmatocystin (ST). Deletion PaStcA encoding polyketide synthase and overexpression (OE) PaAflR ST-specific transcription factor P....
Fungal histidine kinase receptors (HKRs) sense and transduce many extracellular signals. We investigated the role of HKRs in morphogenetic transition, osmotolerance, oxidative stress response, mating ability opportunistic yeast Candida lusitaniae. isolated three genes, SLN1, NIK1, CHK1, potentially encoding classes VI, III, X, respectively. These genes were disrupted by a transformation system based upon "URA3 blaster" strategy. Functional analysis disruptants was undertaken, except for sln1...
We recently characterized the histidine kinase receptor genes of Candida lusitaniae. For present study, we have further investigated role SSK1 and SKN7, encoding response regulators. The results functional analysis mutants indicated that Ssk1p is involved in osmotolerance pseudohyphal development, whereas Skn7p appears crucial for oxidative stress adaptation.
Plant biomass degradation by fungi is a critical step for production of biofuels, and laccases are common ligninolytic enzymes envisioned ligninolysis. Bilirubin oxidases (BODs)-like related to laccases, but their roles during lignocellulose have not yet been fully investigated. The two BODs the ascomycete fungus Podospora anserina were characterized targeted gene deletions. Enzymatic assay revealed that bod1(Δ) bod2(Δ) mutants lost partly thermostable laccase activity. A triple mutant...
Background: Halophilic bacteria are potent organisms in production of novel bioactive antimicrobial compounds which might be considered drug innovation and control plant pathogens. Salt deserts Semnan province the most permanent hypersaline areas North Iran. Despite importance these areas, there is no scientific report regarding biodiversity potency their halophilic bacteria. Thus, aforementioned were selected to detect Methods: Here, seven strains isolated cultured on molecular biochemical...
The nucleotide sequence of the URA3 gene encoding orotidine-5'-phosphate decarboxylase (OMP DCase) human opportunistic pathogen yeast Candida lusitaniae was determined by degenerate PCR and chromosome walking. Deduced amino acid showed strong homologies (59-85% identity) with OMP DCases different Saccharomycetales allowed identification known conserved domains. Very close upstream from gene, 3'-end a Gea2-like protein identified. A non-revertible C. ura3 mutant selected on basis...