A5050655185- Genetics and Neurodevelopmental Disorders
- Genomic variations and chromosomal abnormalities
- Protein Structure and Dynamics
- Hemoglobin structure and function
- DNA and Nucleic Acid Chemistry
- Autism Spectrum Disorder Research
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Prenatal Screening and Diagnostics
- Genetic Neurodegenerative Diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Spectroscopy and Quantum Chemical Studies
- RNA regulation and disease
- Congenital heart defects research
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Toxoplasma gondii Research Studies
- Parasite Biology and Host Interactions
- Animal Ecology and Behavior Studies
- Parkinson's Disease Mechanisms and Treatments
National Institutes of Health
2015-2025
National Institute of Allergy and Infectious Diseases
2022-2025
Musée National d'Histoire Naturelle
2017-2024
University of Göttingen
2017-2024
Center for Information Technology
2008-2020
United States Naval Research Laboratory
2015
National Institute on Alcohol Abuse and Alcoholism
2015
University Hospital Ulm
2000-2014
Landesamt für Landwirtschaft, Lebensmittelsicherheit und Fischerei
2003-2013
United States Department of Health and Human Services
2011
Abstract New atom‐ and group‐based spherical‐cutoff methods have been developed for the treatment of nonbonded interactions in molecular dynamics (MD) simulation. A new atom‐based method, force switching, leaves short‐range forces unaltered by adding a constant to potential energy, switching smoothly zero over specified range. simple improvement cutoffs is presented: Switched group‐shifting shifts group–group energy before being switched zero. Also introduced are generalizations shifting,...
To determine whether Förster resonance energy transfer (FRET) measurements can provide quantitative distance information in single-molecule fluorescence experiments on polypeptides, we measured FRET efficiency distributions for donor and acceptor dyes attached to the ends of freely diffusing polyproline molecules various lengths. The observed mean efficiencies agree with those determined from ensemble lifetime but differ considerably values expected theory, treated as a rigid rod. At...
Quasistatic and kinetic studies of the infrared CO stretch bands carbonmonoxymyoglobin show that proteins glasses share essential characteristics, in particular metastability below a transition temperature relaxation processes are nonexponential time non-Arrhenius temperature.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLigand binding to heme proteins: connection between dynamics and functionPeter J. Steinbach, Anjum Ansari, Joel Berendzen, David Braunstein, Kelvin Chu, Benjamin R. Cowen, Ehrenstein, Hans Frauenfelder, Bruce Johnson, Cite this: Biochemistry 1991, 30, 16, 3988–4001Publication Date (Print):April 23, 1991Publication History Published online1 May 2002Published inissue 23 April 1991https://doi.org/10.1021/bi00230a026RIGHTS & PERMISSIONSArticle...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTProteins and pressureHans. Frauenfelder, Neil A. Alberding, Anjum. Ansari, David. Braunstein, Benjamin R. Cowen, Mi Kyung. Hong, Icko E. T. Iben, J. Bruce. Johnson, Stan. Luck, . Cite this: Phys. Chem. 1990, 94, 3, 1024–1037Publication Date (Print):February 8, 1990Publication History Published online1 May 2002Published inissue 8 February 1990https://pubs.acs.org/doi/10.1021/j100366a002https://doi.org/10.1021/j100366a002research-articleACS...
Molecular dynamics (MD) simulation covering a wide range of hydration indicate that myoglobin is fully hydrated by 350 water molecules, in agreement with experiment. These waters, originally placed uniformly about the protein, form clusters hydrate every charged group throughout entire simulation. Some atoms groups are two layers while 37% protein surface remains uncovered. The locations waters consistent those crystallographic resolved x-ray and neutron diffraction. Hydration at 300 K...
Quenching of the triplet state tryptophan by cysteine provides an important new tool for measuring rate at which a specific intramolecular contact is formed in disordered polypeptides. By viscosity dependence quenching rate, both reaction-limited and diffusion-limited rates can be determined. The corresponds to forming short-range contact. depends solely on equilibrium end-to-end distribution, becomes essentially length-independent short chains, providing clear-cut evidence that chain stiff....
Abstract Altered DNA dynamics at lesion sites are implicated in how repair proteins sense damage within genomic DNA. Using laser temperature-jump (T-jump) spectroscopy combined with cytosine-analog Förster Resonance Energy Transfer (FRET) probes that local conformations, we measured the intrinsic of containing 3 base-pair mismatches recognized vitro by Rad4 (yeast ortholog XPC). Rad4/XPC recognizes diverse lesions from environmental mutagens and initiates nucleotide excision repair. T-jump...
We analyzed the effect of substituting serine for each 19 cysteine residues within amino-terminal extracellular domain human Ca2+ receptor on cell surface expression and dimerization. C129S, C131S, C437S, C449S, C482S were similar to wild type receptor; other 14 mutants retained intracellularly. Four these, C60S, C101S, C358S C395S, unable dimerize. A C129S/C131S double mutant failed dimerize but was unique in that monomeric form expressed at surface. Substitution a 132 restored Mutation...
<b>Objective:</b> Myotonic dystrophy type 1 and 2 (DM1/DM2) are multisystemic diseases with common cognitive deficits beside the cardinal muscular symptoms. We performed a comprehensive analysis of cerebral abnormalities to compare neuropsychological defects findings in different imaging methods same cohort patients. <b>Methods:</b> Neuropsychological investigations, structural MRI including brain parenchymal fraction (BPF) voxel-based morphometry (VBM), <sup>18</sup>F-deoxy-glucose PET...
Different mutations occurring in the unstable CGG repeat 5' untranslated region of FMR1 gene are responsible for three fragile X-associated disorders. An expansion over ∼200 repeats when associated with abnormal methylation and inactivation promoter is mutation termed 'full mutation' X syndrome (FXS), a neurodevelopmental disorder described as most common cause inherited intellectual impairment. The term 'abnormal methylation' used here to distinguish DNA induced by expanded from 'normal on...
Significance HLA alleles have previously been implicated with scleroderma risk, but, in this study, using a European American ancestral cohort and newly recruited large of African Americans, we comprehensively define the amino acid residues associated scleroderma. Scleroderma is characterized by mutually exclusive specific autoantibodies. We demonstrated ancestry-predominant that were much more strongly autoantibody subsets than overall risk SSc. bioinformatically predicted immunodominant...
We report on further cases of high functioning fragile X males showing decreased expression FMR1 protein, absence detectable methylation at the EagI site in gene promoter, and highly unusual patterns mutations defined as smear expansions extending from premutation to full mutation range. Very diffuse therefore not easily were also observed prenatal testing using DNA chorionic villi sampled a time development when still unmethylated this particular tissue. In search for possible determinants...