A5087253339- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Wnt/β-catenin signaling in development and cancer
- Cancer Cells and Metastasis
- Graphene and Nanomaterials Applications
- Boron and Carbon Nanomaterials Research
- Metal and Thin Film Mechanics
- Nanoparticles: synthesis and applications
- Advanced materials and composites
- Dental materials and restorations
- Cell Adhesion Molecules Research
- Hippo pathway signaling and YAP/TAZ
- 3D Printing in Biomedical Research
- Orthopaedic implants and arthroplasty
- Surface Treatment and Coatings
- Graphene research and applications
- Titanium Alloys Microstructure and Properties
- Laser-Ablation Synthesis of Nanoparticles
- Antimicrobial agents and applications
- Microtubule and mitosis dynamics
- Kruppel-like factors research
- Diamond and Carbon-based Materials Research
- Nuclear Structure and Function
- Cancer-related gene regulation
- Dielectric properties of ceramics
University of Toronto
2024
Ministry of Health of the Russian Federation
2017-2023
Université Paris Sciences et Lettres
2019-2020
Institut Curie
2019-2020
Centre National de la Recherche Scientifique
2019-2020
Institut Pierre-Gilles de Gennes pour la Microfluidique
2019-2020
Russian Cancer Research Center NN Blokhin
2009-2020
Russian Academy of Sciences
2010-2011
Academy of Medical Sciences
2010-2011
The nucleus makes the rules Single cells continuously experience and react to mechanical challenges in three-dimensional tissues. Spatial constraints dense tissues, physical activity, injury all impose changes cell shape. How can measure shape deformations ensure correct tissue development homeostasis remains largely unknown (see Perspective by Shen Niethammer). Working independently, Venturini et al. Lomakin now show that act as an intracellular ruler cellular variations. nuclear envelope...
Nanoparticles (NPs) have a great potential as nanosized drug-delivery carriers. Such systems must safely deliver the drug to site of tumor without leakage, effectively penetrate inside cancer cells, and provide intracellular release. Herein we developed an original simple method aimed at fabrication spherical boron nitride NPs (BNNPs), 100–200 nm in diameter, with peculiar petal-like surfaces via chemical vapor deposition. structures were found be able absorb large amount antitumor...
Epithelial-mesenchymal transition (EMT) plays an important role in development and also initiation of metastasis during cancer. Disruption cell-cell contacts EMT allowing cells to detach from migrate away their neighbors remains poorly understood. Using immunofluorescent staining live-cell imaging, we analyzed early events induced by epidermal growth factor (EGF) IAR-20 normal epithelial cells. Control demonstrated stable adherens junctions (AJs) robust contact paralysis, whereas addition...
Due to their good mechanical stability compared gelatin, collagen or polyethylene glycol nanofibers and slow degradation rate, biodegradable poly-ε-caprolactone (PCL) are promising material as scaffolds for bone soft-tissue engineering. Here, PCL were prepared by the electrospinning method then subjected surface functionalization aimed at improving biocompatibility bioactivity. For modification, two approaches used: (i) COOH-containing polymer was deposited on using atmospheric pressure...
E-cadherin–mediated cell–cell adhesion, which is essential for the maintenance of architecture and integrity epithelial tissues, often lost during carcinoma progression. To better understand nature alterations interactions at early stages neoplastic evolution cells, we examined line nontransformed IAR-2 cells their descendants, lines IAR-6-1 transformed with dimethylnitrosamine IAR1170 N-RasG12D. retained E-cadherin, displayed discoid or polygonal morphology, formed monolayers similar to...
Enhanced incorporation of hydroxyapatite nanoparticles in porous titania coating formed by plasma electrolytic oxidation significantly increases surface osteogenic activity.
Herein we study the effect of doxorubicin-loaded BN nanoparticles (DOX-BNNPs) on cell lines that differ in multidrug resistance (MDR), namely KB-3-1 and MDR KB-8-5 cervical carcinoma lines, K562 i-S9 leukemia lines. We aim at revealing possible differences cytotoxic free DOX DOX-BNNP nanoconjugates these types cells. The spectrophotometric measurements have demonstrated maximum amount DOX-BNNPs is obtained after saturation alkaline solution (pH 8.4), indicating high efficiency BNNPs with...
It is very important to prevent bacterial colonization at the early postoperative stages. There are four major strategies and their corresponding types of antibacterial surfaces specifically designed fight infection: bactericide release, anti-adhesion, pH-sensitive, contact-killing. Herein, we aimed determining efficiency different bactericidal ions revealing possible contribution surface microgalvanic effects arising from a potential difference on heterogeneous surfaces. We considered five...
Using confocal microscopy, we analyzed the behavior of IAR-6-1, IAR1170, and IAR1162 transformed epithelial cells seeded onto confluent monolayer normal IAR-2 cells. Live-cell imaging neoplastic stably expressing EGFP mKate2 showed that retaining expression E-cadherin were able to migrate over invade monolayer. Transformed IAR invaded at boundaries between Studying interactions IAR-6-1 GFP-E-cadherin with monolayer, found established E-cadherin-based adhesions cells: dot-like dynamic in...
Drug-loaded nanocarriers have a great potential for tumor therapy. Such systems must high drug-loading efficacy in an alkaline medium and effectively release therapeutic agent acidic of endosomal/lysosomal compartments cells. Herein, we experimentally theoretically (using density functional theory) studied the chemical interaction doxorubicin (DOX) with different boron nitride (BN) surfaces depending on degree their oxidation. Three groups hexagonal BN nanoparticles (BNNPs) obtained by oxide...
Abstract The microscopic environment inside a metazoan organism is highly crowded. Whether individual cells can tailor their behavior to the limited space remains unclear. Here, we found that measure degree of spatial confinement using largest and stiffest organelle, nucleus. Cell below resting nucleus size deforms nucleus, which expands stretches its envelope. This activates signaling actomyosin cortex via nuclear envelope stretch-sensitive proteins, upregulating cell contractility. We...
Epithelial–mesenchymal transition (EMT) is a process during which epithelial cells lose characteristics and gain mesenchymal features. Here, we used several cell models to study migratory activity redistribution of cell–cell adhesion proteins in different EMT states: EGF-induced IAR-20 cells; IAR-6-1 with hybrid epithelial–mesenchymal phenotype; their more derivatives, IAR-6-1-DNE lacking adherens junctions. In migrating cells, the protein α-catenin accumulated at leading edges along...