A5057945723- Glioma Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Renal and related cancers
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Wnt/β-catenin signaling in development and cancer
- Hormonal Regulation and Hypertension
- Lipid Membrane Structure and Behavior
- Testicular diseases and treatments
- MicroRNA in disease regulation
- Ocular Oncology and Treatments
- Pancreatic function and diabetes
- Cancer Cells and Metastasis
- Single-cell and spatial transcriptomics
- Birth, Development, and Health
- Histone Deacetylase Inhibitors Research
- Cancer-related gene regulation
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Adipose Tissue and Metabolism
Harvard University
2021-2024
Boston VA Research Institute
2023
Universidade de São Paulo
2018-2022
Clinics Hospital of Ribeirão Preto
2021
Universidade Federal de Minas Gerais
2017
Abstract Introduction Maternal separation is an early life stress event associated with behavioral alterations and ethanol consumption. We aimed to expand the current understanding on molecular mechanisms mediating impact of postnatal Methods In first experiment (T1), some pups were separated from their mothers for 6 hr daily (Maternal Separation group ‐ MS ), whereas other remained in cage respective (Control C). second (T2), mice both groups subjected model free‐choice between water...
Biomolecular condensates formed by liquid–liquid phase separation have been implicated in multiple diseases. Modulation of condensate dynamics small molecules has therapeutic potential, but so far, few modulators disclosed. The SARS-CoV-2 nucleocapsid (N) protein forms phase-separated that are hypothesized to play critical roles viral replication, transcription, and packaging, suggesting N condensation might anti-coronavirus activity across strains species. Here, we show proteins from all...
Next-generation sequencing platforms are routinely used for molecular assignment due to their high impact risk stratification and prognosis in medulloblastomas. Yet, low middle-income countries still lack an accurate cost-effective platform perform this allocation. TaqMan Low Density array (TLDA) assay was performed using a set of 20 genes 92 medulloblastoma samples. The same methodology assessed silico microarray data 763 samples from the GSE85217 study, which MB classification by robust...
Significance The Hedgehog signaling pathway is fundamental in animal development, controlling the size, morphology, and differentiation of tissues organs. How evolutionarily tuned to produce different outputs poorly understood. We use cell biology, mathematical modeling, analysis mutations causing birth defects demonstrate that ultrasensitivity a key signal-processing feature pathway, which can lead outputs. explain by model whereby tumor-suppressor membrane protein Patched catalyzes...
The steroid hormone aldosterone, produced by the zona glomerulosa (zG) of adrenal gland, is a master regulator plasma electrolytes and blood pressure. While aldosterone control renin-angiotensin system well understood, other key regulatory factors have remained elusive. Here, we replicated prior association between non-coding variant in
BackgroundSupratentorial RELA fusion (ST-RELA) ependymomas (EPNs) are resistant tumors without an approved chemotherapeutic treatment. Unfortunately, the molecular mechanisms that lead to chemoresistance traits of ST-RELA remain elusive. The aim this study was assess fusion-dependent signaling modules, specifically role Hedgehog (Hh) pathway as a novel targetable vulnerability in ST-RELA.
Abstract Coronavirus-infected cells contain double-membrane vesicles (DMVs) that are key for viral RNA replication and transcription, perforated by hexameric pores connecting the vesicular lumen to cytoplasm. How form traverse two membranes, how DMVs organize synthesis, is unknown. Using structure prediction functional assays, we show non-structural membrane protein nsp4 DMV pore organizer, spanning double forming most of lining. Nsp4 interacts with nsp3 on cytoplasmic side replicase inside...
Abstract Disclosure: K.S. Borges: None. D.W. Little III: T. de Almeida Magalhães: C. Ribeiro: K. Basham: Dumonte: S. Azova: A.E. O’Connell: N.A. Guagliardo: P.Q. Barrett: M. Berber: D. Mohan: A. Turcu: W. Rainey: A.M. Lerario: D.L. Carlone: Salic: D.T. Breault: G.D. Hammer: The steroid hormone aldosterone, produced by the zona glomerulosa (ZG) of adrenal gland, plays a key role in regulating plasma electrolyte levels and blood pressure. ZG dysregulation, major driver primary aldosteronism...
Coronavirus-infected cells contain double-membrane vesicles (DMVs) that are key for viral RNA replication and transcription, perforated by hexameric pores connecting the vesicular lumen to cytoplasm. How form traverse two membranes, how DMVs organize synthesis, is unknown. Using structure prediction functional assays, we show nonstructural membrane protein nsp4 pore organizer, spanning double forming most of lining. Nsp4 interacts with nsp3 on cytoplasmic side replicase inside DMV. Newly...
Advances in genomics have led to the identification of twelve relevant molecular subtypes within medulloblastoma (MB). The alpha subtype Sonic hedgehog-driven MB is resistant therapy (including smoothened inhibitors) due activation genes from non-canonical SHH pathway, such as MYCN, YAP1, or TP53. Using retrospective cohort microarray data, we found that YAP1 overexpressed and patients profiled SMO inhibitors compared good responders. Here, performed depletion via CRISPR/Cas9 two vitro...
Introduction Ependymoma (EPN) is the third most common childhood cancer of central nervous system. RELA subgroup EPN accounting for approximately 70% all supratentorial tumours and shows worst prognosis among EPNs. Due to low efficacy chemotherapy treatment options this tumour are limited. Sonidegib (LDE225) a Sonic Hedgehog (SHH) pathway inhibitor (via smoothened antagonism) it under Phase II study in paediatrics brain (e.g.medulloblastomas). However, there no studies describing role SHH...
Abstract Background: Medulloblastoma (MB) is the most common malignant brain tumor in childhood. Despite improvement current treatment, children still suffer from long-term sequelae resulting standard care therapy. World Health Organization considers MB as 4 distinct entities: SHH, WNT, Group 3, and 4. The aggressive variant of SHH bearer TP53 mutation it resistant to chemo/radiation Therefore, novel treatments modalities are urgently needed. In this study we aimed evaluate biologic...
Abstract Introduction: Recently we found that RELA ependymomas (EPN) demonstrate inappropriate activation of the Hedgehog (Hh) pathway. However, how this is modulated remains to be investigated. Primary cilia are essential positive and negative modulation Hh signaling changes in ciliation seem linked drug resistance pediatric cancers. In contrast, targeting integrity by molecules prevents ciliary disassembly, such as Aurora-A (AURKA), has been shown overcome resistance. role pathway...
Abstract Biomolecular condensates formed by liquid-liquid phase separation have been implicated in multiple diseases. Modulation of condensate dynamics small molecules has therapeutic potential, but so far, few modulators disclosed. The SARS-CoV-2 nucleocapsid (N) protein forms separated that are hypothesized to play critical roles viral replication, transcription and packaging, suggesting N condensation might anti-coronavirus activity across strains species. Here, we show proteins from all...